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Citations for Cell Signaling Technology :
2751 - 2800 of 4577 citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 100 μg of brain protein lysates were incubated with 4 μg of anti-RIPK1 (Cell signaling) for 48 hours while rotating at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibodies against phosphorylated and total proteins were purchased from Cell Signaling Technology (Andover, MA). Custom mouse monoclonal antibodies were made against EGFR L858R that recognizes mutant EGFR ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were stained using primary antibodies against IFITM1 (α-IFITM1, Cell Signaling Cat#13126S, 1:1,000), IFITM2 (α-IFITM2 Cell Signaling Cat#13530 S ...
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bioRxiv - Plant Biology 2019Quote: ... Protein extraction and immunoblotting with an antibody against phosphorylated MAPKs (p44/42; 9101; Cell Signaling Technology) was then performed ...
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bioRxiv - Pathology 2020Quote: ... Fatty acid binding protein (FABP)-4-specific rabbit monoclonal antibody (1:1000 dilution, D25B3, Cell Signaling), BNIP3 specific rabbit monoclonal antibody (1:1000 dilution ...
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bioRxiv - Genetics 2021Quote: ... The expression of NQO1 protein were detected using NQO1 monoclonal antibody (Cell signaling technologies, Beverly, MA). After post-primary blocking the sections were incubated with Novolink polymer and were then developed with DAB using supplied substrate buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein samples (30 µg) obtained from lysis in RIPA buffer (Cell Signaling Technology, Danvers, MA, USA) of infected cells were separated on 10% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: ... protein A magnetic beads (73778S) and streptavidin magnetic beads (5947S) were purchased from Cell Signaling Technologies. Primary antibody for c-Myc (sc-40 ...
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bioRxiv - Systems Biology 2021Quote: ... 1: 1000 anti-Phopho-S6 ribosomal protein (Ser240/244) (D68F8) Rabbit mAb (Cell Signaling Technology, 5364), 1:1000 anti-S6 Ribosomal Protein (5G10 ...
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bioRxiv - Neuroscience 2020Quote: ... or phopsho-ribosomal S6 protein (PS6; Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA). The secondary antibody ...
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bioRxiv - Physiology 2020Quote: ... For rabbit anti-p-CREB (cAMP response element-binding protein) primary antibody (1:2000, Cell Signaling), aminomethylcoumarin (AMCA)-conjugated donkey anti-rabbit secondary antibody (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... and signal (protein expression) was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Cell Signaling, 1:1000).
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bioRxiv - Cell Biology 2021Quote: ... We used 10 μg protein per lane and incubated with primary antibodies (Cell Signaling Technology, CST) and secondary antibodies (Dako ...
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bioRxiv - Bioengineering 2022Quote: ... while the absence of contaminating proteins were confirmed using antibodies for calnexin (Cell Signaling Technology, 2679), HSP90 (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... while the absence of contaminating proteins were confirmed using antibodies for GAPDH (Cell Signaling Technology, 2118) and calnexin (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... Target proteins were probed with the following antibodies: AMPKα (1:1,00, Cell Signaling Technology, #2532, RRID:AB_330331), pAMPK (T172 ...
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bioRxiv - Cell Biology 2022Quote: ... Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E, Alexa Fluor 555, 1:200, Cell Signaling Technology, 39855); S6 Ribosomal Protein (54D2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP Rabbit mAb (Cell Signaling Technology, Cat# 4858, RRID:AB_916156) at 1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein levels were detected by specific antibodies directed against: Human EVI1 (Cell signaling, cat 2265), CTBP1 (BD ...
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bioRxiv - Neuroscience 2023Quote: ... total and phosphorylated (Ser235/236) S6 ribosomal protein and pULK1 (Ser757) (all 1:1000, Cell Signaling). Bands detected with secondary antibodies coupled to peroxidase and enhanced chemiluminescent reagent were captured in ChemiDoc MP System and quantified in a linear range using the ImageLab 5.2.1 software (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitated proteins were thereafter analysed by western blot using monoclonal anti-MSK1 antibody (#3489; Cell Signaling) 1:1000 and polyclonal antibodies anti-MeCP2 (10861-1-AP ...
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bioRxiv - Molecular Biology 2024Quote: The following primary antibodies were used: anti-HSP70 family proteins (Cell Signaling Technology, #4872; 1:1000) and anti-β-actin (LI-COR Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198 ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used: a rabbit Mab S6 ribosomal protein (Cell Signaling®: 2217) and a rabbit Mab Phospho-S6 ribosomal protein (Ser235/236 ...
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bioRxiv - Microbiology 2023Quote: ... rabbit antibodies recognizing cellular proteins (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411 ...
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bioRxiv - Genetics 2023Quote: ... The chromatin DNA-protein complexes were incubated with an anti-MeCP2 antibody (Cell Signaling Technology, 3456) or an isotype control antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-HA clone C29F4 (1/1000 dilution for brain membrane protein preparations, Cell Signaling # 3724S), NeuroMab mouse anti-NaV1.1 clone K74/71 (1/300 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Protein bands were detected by exposing the membrane to SignalFire™ ECL substrate (Cell Signaling Technology) on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... The ChIP-grade magnetic beads coated with protein G were purchased from Cell Signaling Technology (USA). The antibodies used in immunoprecipitation were Pol II (#39097 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were analyzed by SDS-PAGE and immunoblotting using p44/42 MAPK antibody (Cell Signaling Technology).
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... followed by HRP-linked protein A (1:10000 dilution in TBST; #12291, Cell Signaling Technology, USA). HRP-linked protein A is useful for preventing immunoprecipitation artifacts ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were captured by addition of HIF-1α (D1S7W) XP® Rabbit mAb (Cell Signaling Technology) and incubated overnight at 4°C with rotation followed by immunoprecipitation by addition of Protein A magnetic beads and incubation for 20 minutes at RT ...
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bioRxiv - Genetics 2021Quote: ... containing 5% (w/v) skimmed dried milk powder and immunoblotted using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody (#2851 Cell Signaling) (dilution 1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were dissociated into single cells by TlypLE select and suspended in PBS containing the DNA dye DRAQ-7 (Cell Signaling). Using FACS Aria IIIu flowcytometry ...
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bioRxiv - Biophysics 2022Quote: ... was added for 45 min then replaced by PBS + 1% BSA containing HA antibody 1:1000 (12CA5, Santacruz) or Myc antibody 1:1000 (D84C12, Cell Signaling) for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... Samples containing 10 µg of DNA were immunoprecipitated with 375 ng (10 µl) of TCF7L2 antibody (Cat. no. 2569, Cell Signaling). Incubation with protein G agarose beads and elution of precipitated chromatin was conducted according to ChIP-IT High Sensitivity kit protocol (Cat ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed three times with TBS containing 0.1% Tween 20 then incubated with anti-Rabbit IgG-HRP conjugated (Cell Signaling: 7074). Images were acquired by chemiluminescence using Clarity ECL (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were harvested after 24 hours by trypsinization and washed twice with cold PBS containing 10 nM calyculin A (Cell Signaling). Cells were sorted from both the adGFP and adGREB1 co-cultures using a Becton Dickinson FACSAria II cell sorter into GFP-positive (transduced ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated in a blocking solution containing phospho-AMPKα Thr 172 (Cell Signaling Technologies, Cat # 2535S, 1:1000 dilution) or β-actin (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then incubated o/n at 4C with 5% milk powder in PBS-T containing the primary antibodies at the following dilutions (1:1000 Lin28a (A177, Cell signaling); 1:4000 H3 (Abcam ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed by ice-cold RIPA buffer (150mM NaCl, 1% NP40, 0.1% SDS and 50mM Tris, PH7.4) containing protease and phosphatase inhibitor (Cell Signaling Technologies; 5872) for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 10% non-fat dry milk powder in tris-buffered saline containing 0.1% Tween 20 (TBST) for two hours and then incubated with an anti-phosphoCRKL antibody (Cell Signaling Technology) and Anti β –Actin antibody (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and the grey matter was Dounce homogenized in 5 volumes per weight (v/w) buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Evolutionary Biology 2023Quote: Western blotting: Cells were harvested and lysed by adding 350 μL of lysis buffer containing 1× cell lysis buffer (Cell Signaling), 1 mM PMSF (Cell Signaling) ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min and after destaining the membranes were incubated at 4°C over night with primary antibodies in the respective concentration using 1xTBS containing 0.1% Tween 20 including LC3 A/B (Cat. No. #4108S, dilution 1:1000 in blocking solution, Cell Signaling Technology), p62 (Cat ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed 3X in PBS and blocked for 1 h at RT with 5% BSA solution containing 5% normal goat serum (Cell Signaling) and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... were blocked in TBST buffer (Tris-buffered saline and 0.15% Tween-20) containing 5% nonfat dry milk (#9999, Cell Signaling Technology). The blots were probed overnight with primary antibodies diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were stained for β-galactosidase using the activity kit (Kit 9860S) from Cell Signaling Technologies according to the manufacturer’s protocol ...