Labshake search
Citations for Cell Signaling Technology :
2601 - 2650 of 3369 citations for Mouse Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
-
bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
-
bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
-
bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
-
bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
-
bioRxiv - Microbiology 2024Quote: ... the ADP-ribosylated proteins were visualized using chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
-
bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
-
bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
-
bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
-
bioRxiv - Cancer Biology 2023Quote: ... or EV fraction was loaded alongside a biotinylated ladder and/or pre-stained protein molecular weight markers (Catalog numbers 7727 and 59329, respectively, Cell Signaling Technology) on NuPAGE™ Bis-Tris™ pre-cast gels (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were stained at 1:100 with anti-phospho S6 ribosomal protein (Ser235/236) Alexa Fluor 647 (D57.2.2E, Cell Signaling Technologies cat#4851S).
-
bioRxiv - Cell Biology 2024Quote: ... the resulting supernatants were incubated for 3 hours at 4°C with a 2.5 µL slurry of Sera-Mag SpeedBeads Protein A/G (Cytiva) pre-incubated with 2.5 µL of anti-HA-tag rabbit mAb (Cell signaling technology, C29F4). The beads were washed four times with the lysis buffer and then twice with 50 mM ammonium bicarbonate ...
-
bioRxiv - Cell Biology 2024Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
-
bioRxiv - Biochemistry 2024Quote: ... which were blocked by 5% non-fat milk (americanBIO, AB10109-01000) and then incubated overnight at 4°C with the following primary antibodies: Nucleocapsid protein (Cell Signaling, #33717), Strep-tagged Nucleocapsid protein (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT# ...
-
bioRxiv - Neuroscience 2024Quote: ... K-ε-GG peptide enrichment was performed using the K-ε-GG-specific antibody (PTM-Scan HS Ubiquitin/SUMO remnant motif K-ε-GG kit, Cell Signaling Technology, Kit#59322) covalently linked to magnetic beads ...
-
bioRxiv - Neuroscience 2024Quote: K-ε-GG peptide enrichment was performed using a K-ε-GG-specific antibody (PTM-Scan ubiquitin remnant motif K-ε-GG kit, Cell Signaling Technology, Kit#5562) chemically crosslinked to agarose beads as previously described (Udeshi et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... and diaminobenzidine substrate kit (#8059, Cell Signaling Technologies, Beverly, MA, USA) were used for immunohistochemistry ...
-
bioRxiv - Cancer Biology 2019Quote: ... anti-GAPDH (sampler kit from Cell signaling technology, US; 1:1000), horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (all purchased from Cell Signaling Technology) ...
-
bioRxiv - Neuroscience 2019Quote: RapGAP activities were assessed using Active Rap1 detection kit (Cell Signaling) (82 ...
-
bioRxiv - Cell Biology 2020Quote: BrdU Cell Proliferation Assay Kit (Cell Signaling Technology, Danvers, MA #6813) was performed as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... A DAB substrate kit (8059) was purchased from Cell Signaling Technology. Opti-MEM I reduced-serum medium (51985-034 ...
-
bioRxiv - Developmental Biology 2021Quote: ... the SignalStain DAB substrate kit was used (Cell Signaling Cat# 8059) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... PTMScan Ubiquitin Remnant Motif Kit (Cell Signaling Technology, Cat. 5562, USA), C18 Sep-Pak SPE cartridge (Waters ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the PTMScan Acetyl-Lysine Motif Kit (Cell Signaling Technology, 11663V1). The flow-through of the Acetyl-Lysine immunoprecipitation was kept for phosphorylated peptide enrichment ...
-
bioRxiv - Cell Biology 2023Quote: ... We utilized the SimpleChIP Enzymatic Chromatin IP kit from Cell Signaling following the manufactures protocol with a few exceptions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell viability was measured using XTT assay kit (Cell Signaling Technology) using a 96-well format.
-
bioRxiv - Molecular Biology 2023Quote: ... A senescence β-galactosidase staining kit (Cell Signaling Technology, Cat# 9860) was employed to score for senescence ...
-
bioRxiv - Cancer Biology 2023Quote: ... PTMScan Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, 13416), GSH/GSSG detection assay (Beyotime ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then developed with a DAB substrate kit (Cell Signaling, #8059).
-
bioRxiv - Cell Biology 2024Quote: ... the Bromodeoxyuridine (BrdU) Cell Proliferation Assay Kit (#6813, Cell Signaling Technology), resazurin reduction (alamarBlue™ Cell Viability Reagent ...
-
bioRxiv - Microbiology 2024Quote: ... Autophagy Antibody Sample Kit (4445T) was purchased from Cell Signaling Technology. Lyso-Tracker Red (C1046) ...
-
bioRxiv - Cancer Biology 2021Quote: ... membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling ...
-
bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
-
bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
-
bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
-
bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...