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Citations for Cell Signaling Technology :
2601 - 2650 of 2715 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 8% goat serum and incubated overnight at 4°C with anti-phospho Axl (R&D, AF2228, diluted 1:200) CK11 (Cell signaling, CST 4545, diluted 1:200), followed by fluorescently labelled secondary antibodies goat anti mouse 488 (Abcam ab98637) ...
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bioRxiv - Genetics 2019Quote: ... Coated wells were blocked with 5% non-fat milk and 0.1% Tween 20 and then incubated with anti-phospho-Ser22-Lamin A/C antibody (Cell Signaling 13448S, Lot 1, 1:1000 dilution) or the anti-pan-N-terminal-Lamin A/C antibody (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated at 37°C for 2 h with the corresponding secondary antibody: horseradish peroxidase (HRP)-linked anti-mouse IgG antibody (#7076, Cell Signaling Technology [CST]; diluted 1:2000) or HRP-linked anti-rabbit IgG antibody (#7074 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were carried out on the remaining sample overnight at 4 °C with rotation with 5 uL of antibody (Cell Signaling Technology D31H2 anti-CTCF, 3418S). 50uL of Protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Supernatant was transferred to new tubes and antibody was added and allowed to bind overnight at 4 °C with rotation (15uL antibody per tube, Cell Signaling Technology D31H2 anti-CTCF, 3418S). 30uL of pre-washed Protein G Dynabeads were added to each tube and rotated at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% milk in 1X TTBS and incubated overnight with the appropriate primary antibody (Cell Signaling c-IAP2 58C7 Rabbit mAb #3130; Cell Signaling XIAP Antibody #2042; Cell Signaling Mcl-1 (D2W9E) Rabbit mAb #94296 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and incubated with a 30 μl bed volume of protein-A-sepharose beads and 1.5 μl of α-IGF-1 receptor β (D23H3) XP (Cell Signaling) per sample ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then blotted overnight with anti-human phospho-Akt and anti-human phospho-Erk1/2 mAbs (Cell Signaling, MA, USA), followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... and rabbit monoclonal vinculin antibody (clone E1E9V; #13901, 1:1000; epitope: amino terminus of human vinculin protein) were purchased from Cell Signaling Technology.
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Microbiology 2020Quote: ... 5 mg of tissue from distal ileal mucosal scrapings was lysed in 250 μL of ice-cold protein lysis buffer (Cell Signaling Technology Cell Lysis Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein level could also be measured by immunofluorescence using fluorescently labeled antibodies (anti-Flag-Alexa 647, #15009; Cell Signaling, Danvers, MA, USA). These antibodies were flowed into the device and incubated with the immobilized proteins under the ‘button’ for 20 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Biochemistry 2019Quote: ... in tris-buffered saline Tween 20 (TBS-T) for 1 h at RT and then blotted for phosphorylated proteins (Cell Signaling Technology; phospho-EGFR Y1068 #3777 ...
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bioRxiv - Molecular Biology 2020Quote: ... the rabbit monoclonal anti-Actin antibody (12748S, rabbit monoclonal antibody to a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein, Cell Signaling) and the rabbit monoclonal anti-acetyl-histone H3 (Lys9 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of PSMA protein in each cell line was investigated by western blot using anti-PSMA rabbit antibody (Cell Signaling Technology) [11].
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Microbiology 2024Quote: ... the ADP-ribosylated proteins were visualized using chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... or EV fraction was loaded alongside a biotinylated ladder and/or pre-stained protein molecular weight markers (Catalog numbers 7727 and 59329, respectively, Cell Signaling Technology) on NuPAGE™ Bis-Tris™ pre-cast gels (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained at 1:100 with anti-phospho S6 ribosomal protein (Ser235/236) Alexa Fluor 647 (D57.2.2E, Cell Signaling Technologies cat#4851S).
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of SARS-CoV-2 nucleocapsid protein (NP) (E8R1L) fragmented mouse monoclonal antibody f-mAb (Cell Signaling Technology Inc., Massachusetts, USA) are introduced to the sensor surface after diluting in PBS and incubated for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the resulting supernatants were incubated for 3 hours at 4°C with a 2.5 µL slurry of Sera-Mag SpeedBeads Protein A/G (Cytiva) pre-incubated with 2.5 µL of anti-HA-tag rabbit mAb (Cell signaling technology, C29F4). The beads were washed four times with the lysis buffer and then twice with 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... At least 1 mg of proteins were immunoprecipitated with mouse anti-Ezrin (Novex, 357300) and rabbit anti-EGF receptor (Cell Signaling 4267) in rotation at 4° C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT# ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked for 10 minutes in 10% BSA in TBS-T and then incubated overnight at 4°C with the indicated antibodies obtained from Cell Signaling Technology (phospho-PKC Substrate Motif (#6967), phospho-p42/44 MAPK (#4370) ...