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Citations for Cell Signaling Technology :
2451 - 2500 of 10000+ citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and blots were then incubated in primary antibodies [anti-PARP1 (Cell Signaling 9542, 1/750), anti-PAR (Enzo Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-linked secondary mouse or rabbit antibody (Cell Signaling Technology; Danvers, MA; 1:5,000 dilution) and ECL detection reagents (cat # K-12045-D20 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the Goat anti-Rabbit secondary antibody (Cell Signaling Technology, 7074S, 1:2000 dilution). Western blot analysis with anti-H3 (Abcam ...
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bioRxiv - Immunology 2024Quote: Anti-HA (3724) and anti-Caspase-1 (2225) antibodies were purchased from Cell Signaling Technology. Anti-FLAG antibody (F1804) ...
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bioRxiv - Genetics 2019Quote: ... The culture medium was then replaced with medium containing 50 mM biotin and cultured for a further 24 hours when the cells were lysed using RIPA Buffer (Cell Signaling Technology) with 1 µM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) rabbit antibody (no. 3674, Cell Signaling Technology, 1:50 dilution); anti-E-cadherin rabbit antibody (no ...
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bioRxiv - Biochemistry 2019Quote: ... The following secondary antibodies were used: horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody and HRP-conjugated anti-rabbit IgG antibody (1:3000 dilution, Cell Signaling Technology, Beverly, Massachusetts, USA). HRP-dependent luminescence was developed with Western Lightning Chemiluminescent Reagent Plus (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... every sixth section of the LPB was incubated in a primary antibody solution containing rabbit anti HA-Tag (C29F4) monoclonal antibody (1:1000; 3724s, Cell Signaling Technologies, Danvers, MA, USA) overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualised using the following primary and secondary antibody combinations: Cyclin B1 was detected with a monoclonal rabbit antibody (Cell Signaling Technology, 12231S, 1:800) and visualised using a goat anti-rabbit Alexa Fluor 647 secondary antibody (Molecular Probes ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibody incubation was carried out overnight in OBB 1:1 in PBS with the anti phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit monoclonal antibody (#4370 Cell Signaling technology. Used at 1:2000 dilution), the monoclonal anti GFP antibody ((0.4mg/ml) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated for 48 hr at 4C in 1× PBS containing the primary antibodies (Chicken anti-GFP, AbCam cat. No. #13970. 1:2000; Rabbit anti-NeuN, Cell Signaling Technology, cat. No. #24307, 1:1000). Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cell Biology 2024Quote: ... at room temperature for 1 h and then incubated for 16 h with primary antibodies against the ER tracker protein disulfide isomerase (PDI; rabbit mAb #3501, Cell Signaling Technologies, Danvers, MA, USA) and a loading control GAPDH (rabbit mAb #2118 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following primary antibodies for 1 hour at RT: rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S), mouse anti-SMA 1:300 (Sigma A5228 ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed with chilled FACS buffer (DPBS with 1% FBS and 2mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hour at room temperature before transferred to the anti-pH3 antibody solution (1:200 in blocking solution; #9706, Cell Signaling; RRID:AB_331748) at 4°C for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... the same amount of protein digestion products was subjected to Western blotting analysis probing with PEP7h antibody (1:5000) or Gapdh-HRP (D16H11, 1:5000, Cell Signaling Technology).
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal SDHA (#11998, 1:1000) and rabbit monoclonal COXIV (3E11 clone, #4850, 1:1000) antibodies were purchased from Cell Signaling Technology. The donkey anti-rabbit (NA934 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cancer Biology 2022Quote: The following antibodies were used for immunoblotting (IB) at 1:1,000 unless otherwise stated: Anti-AXL (8661S, Cell Signaling, IHC 1:500), anti-phospho AXL y702 (5724 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000 ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK1/2 (#9102, Rabbit, WB 1:1000) and MEK1/2 (#4694, Mouse, WB 1:1000) antibodies were purchased from Cell Signaling Technology, MA ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% Tween-20) and incubated 1 hour at room temperature with 1/2500 of primary antibody anti-pERK (Cat.#4377; Cell Signaling Technology) diluted saline buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1 h incubation with the HRP-conjugated secondary antibody (Cell Signaling 7076S, 1:10,000 in 2.5% milk in TBS-T). After three washes in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... p-S6S235/236 (#2211; 1:500) and p-4EBP1T37/46 (#2855; 1:1000) antibodies were obtained from Cell Signaling (Danvers, MA, USA). Cre antibody (NB100-56135 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... Membranes were washed in TBST and incubated 1 hour at room temperature with secondary antibody diluted 1:5000 in 5% milk / TBST [anti-rabbit IgG HRP (Cell Signaling 7074S)] ...
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bioRxiv - Systems Biology 2023Quote: ... rinsed in wash buffer and incubated for one hour in the primary antibodies (PAK4 Santa Cruz Biotechnology sc-390507 1:75 primary dilution, TGFBI Abcam ab170874 1:300 primary dilution, DDR1 Cell Signaling #5583 primary dilution 1:2000 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The sections were incubated with primary antibody (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...
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bioRxiv - Immunology 2023Quote: ... blocked with 5% fat-free milk in TBST and probed with a 1:1000 dilution of an antibody that recognizes the doubly-phosphorylated TGY motif of PMK-1 (Cell Signaling Technology) or a monoclonal anti-tubulin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195 ...