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Citations for Cell Signaling Technology :
201 - 250 of 1336 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used to check the expression level of CBP and P300 by Western blot using anti-P300 and anti-CBP antibodies (Cell Signaling). Anti–β-tubulin antibody (Hybridoma bank # AB_2315513 ...
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bioRxiv - Evolutionary Biology 2023Quote: Western blotting: Cells were harvested and lysed by adding 350 μL of lysis buffer containing 1× cell lysis buffer (Cell Signaling), 1 mM PMSF (Cell Signaling) ...
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bioRxiv - Physiology 2022Quote: Western blotting was performed as previously described [26] using RIPA lysis buffer (Cell Signaling Technology) with added protease and phosphatase inhibitors (Cell Signaling Technology) ...
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bioRxiv - Cancer Biology 2021Quote: ... The experiment of western blot analysis involved the use of antibodies specific for androgen signaling and β-actin as housekeeping antibody (Cell signaling). The standard system of enhanced chemiluminescence was used to visualize the signals ...
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bioRxiv - Molecular Biology 2022Quote: ... Successful separation of subcellular compartments was confirmed by western blot with antibodies against markers of the different compartments: cytoplasmic α-Tubulin (AB_1904178, Cell Signaling Technology), nucleoplasmic Nucleolin (AB_533406 ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and KC in the cell lysates and the culture supernatants were determined by western blot analysis using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control with specific antibodies (Cell Signaling Technology) as previously described (19).
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bioRxiv - Cell Biology 2019Quote: Western blots for quantifying the Twitch-2B concentration were probed with a primary antibody against GFP (Cell Signaling Technology, Beverly, MA; #2555); this antibody also recognizes CFP and YFP ...
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bioRxiv - Cell Biology 2020Quote: ... (3674, 1:1000), RhoE (3664, 1:1000), and phospho-AKT (Ser473) (4060, 1:2000, for Western blot) were from Cell Signaling Technology. Antibodies against phospho-AKT (Thr308 ...
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bioRxiv - Cell Biology 2022Quote: ... and then subjected to western blot analysis using anti-Prdx5 (1:500, Ab Frontier) and anti-hnRNPK (1:500, Cell Signaling Technology) antibodies.
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bioRxiv - Cancer Biology 2020Quote: ... The reaction mixture was resolved by SDS-PAGE and cMYC translation was checked by Western blot analysis using cMYC antibody (Cat# 5605S, Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... Efficacy of individual FXR1 shRNA construct was checked by Western blot analysis for FXR1 knockdown using FXR1 antibody (Cat#12295, Cell Signaling Technology). The most effective shRNA construct was used for generating FXR1 knockdown stable cell line by selection with puromycin (8 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Microbiology 2019Quote: ... The inhibition of Akt activity was confirmed by western blot analysis with rabbit antibody against phospho-S473-Akt (#9271, Cell Signaling Technology), rabbit antibody against Akt (pan ...
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bioRxiv - Systems Biology 2020Quote: ... Figure S5 shows the knockdown efficiency of siRNA transfections in each cell line with GAPDH siRNA compared to no siRNA (just N-TER transfection reagent) and negative control siRNA by Western blot (GAPDH antibody: Cell Signaling Technology, Cat#2118 ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of PSMA protein in each cell line was investigated by western blot using anti-PSMA rabbit antibody (Cell Signaling Technology) [11].
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bioRxiv - Immunology 2020Quote: ... The purity of recombinant Bl-Eng2 was assessed by SDS-PAGE and western blot using an anti-His antibody (Cell signaling Technology).
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bioRxiv - Biophysics 2022Quote: ... B) Western blot of DMSO and dTAG-13 treated WT HeLa cells and TCOF1 degron cells using HA antibody (Cell Signaling (C29F4), 3724S ...
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bioRxiv - Plant Biology 2022Quote: ... Protein electrophoresis and Western-Blot were performed as described previously using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in Western blot assays in xenograft pharmacodynamic (PD) studies: SYK (AB_2687924) (rabbit mAb, Cell Signaling Technology, Cat# 13198, 1:1000), EGFR (AB_2230881 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the western blot was carried out (Odyssey® system) with Anti-Flag (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling). Beta-actin (PA1-183 ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell lysates were analysed by western blot with anti-Gli1 (L42B10) mouse monoclonal antibody (1:800, 2643, Cell Signaling Technology, RRID: AB_2294746) followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and XPO1 and Keap1 proteins were detected via western blot using antibodies against XPO-1/CRM1 (D6V7N, rabbit mAb) andKEAP1 (D1G10) Rabbit mAb (both Cell Signaling Technologies). For competition experiments ...
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bioRxiv - Pathology 2023Quote: Western blot analysis was performed using whole liver lysates for HNF4α (Perseus Proteomics, PP-H1415-00) and cMyc (Cell Signaling Technology, 56055), as described previously [7] ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Clones with successful deletions based on the PCR result were then analyzed using Western blots with antibodies against FOS or EGR1 and pERK antibody (Cell Signaling Technology) to identify clones that lack expression of the respective TFs and still induce MAPK signaling when the RAF1-CR3 kinase domain is activated with 4OHT ...
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bioRxiv - Cell Biology 2023Quote: ... Lack of TLR2 expression in the negative population was confirmed by western blot using a different antibody (Cell Signaling Technology-CST, 13744). Re-expression of mTLR2 or its mutants was achieved by transduction of negative cells with retrovirus carrying the relevant constructs and repeated sorting of the positive population using the aforementioned method ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies for western blots were diluted as follows: rabbit anti-Phospho-AKT Ser473 (D9E) (Cell Signaling Technology, USA; RRID: AB_2797780; 1:2000), mouse anti-Pan-AKT (40D4 ...
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bioRxiv - Neuroscience 2020Quote: ... Western Blot was performed using an anti-Scn2a antibody (Alomone, Jerusalem, Israel) and a β-actin control (Cell Signaling Technology, Danvers, MA, USA).
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bioRxiv - Microbiology 2020Quote: ... Arf6 excision in endothelial cells was verified by isolating lung endothelial cells as previously described (28) and performing a Western blot to assess ARF6 levels using anti-ARF6 antibody from Cell Signaling Technology (#3546) (S5 Figure B).
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bioRxiv - Cancer Biology 2019Quote: ... 80 μg of total cell lysates were analyzed by Western blot using the following antibodies and concentrations: LRP6 (1:1000, Cell Signaling Technology 3395), LRP5 (1:1000 ...
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bioRxiv - Genomics 2022Quote: ... and 5 μg of proteins from each sample were used for western blot. All antibodies (e.g. anti eIF2α, anti Phospho-eIF2α) used were purchased from Cell Signaling Technologies (Table S2). XCell II Blot module was used for wet electrophoretic transfer of proteins from SDS-PAGE gel to nitrocellulose membrane (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl of eluates were loaded on SDS-PAGE gels for western blot analysis using the following antibodies: anti-HA Tag (Cell Signaling Technology, #3724), anti-YWHA (pan ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5), rabbit-α-GAPDH antibodies (Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... then either stained with Coomassie blue or subjected to western blot analysis using a 1:1000 dilution of mouse anti-GFP or mouse anti-Myc antibodies (Cell Signaling Technology, USA). After addition of a 1:5000 dilution of horseradish peroxidase-conjugated horse anti-mouse secondary antibodies (Catalog #7076 ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were performed as described previously (Ji et al., 2019; He et al. 2021) using antibodies against β-catenin (Cell Signaling, Cat#9581), WDR77 (SCBT ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cancer Biology 2022Quote: ... The following primary antibodies were used for western blot in this study: anti-Notch1 (D1E11, Cell Signaling, or mN1A, Novus, or C-20, SCBT) for Notch1 full length and cleaved forms ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... The expression of arrestin-3 and GPCRs was determined by western blot using anti-arrestin F431 [32] anti-HA (#3724, Cell signaling Technology, Danvers, MA) antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... The following antibodies were used for Western blots with the indicated dilution factors: Hexokinase I (C35C4) Rabbit mAb (Cell Signaling Technology, Cat# 2024, RRID:AB_2116996) at 1:1,000 ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of MBP-FoxP3ΔN was validated by Western blot using Mouse MBP Tag antibody(8G1) (Cell Signaling Technology, Cat#2396, 1:2000 dilution).
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2020Quote: ... The IP product was then run on a Western blot and probed using both the rabbit anti-mouse panAkt (1:1000) and Phosopho-Akt (Ser473, Cell Signaling Technologies 4060; 1:1000) antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were then incubated at 28°C and the samples were collected at the indicated intervals for determination of GST and GST-WL1 protein abundance by western blots using the anti-GST (#2625, Cell Signaling Technology, 1:1000 dilution) and alkaline phosphatase-conjugated anti-rabbit (# 31340 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatants (10 μL) were analyzed by Western Blot with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling, 1679101S; dilution 1:1,000) primary antibodies ...