Labshake search
Citations for Cell Signaling Technology :
201 - 250 of 7932 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
-
bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The membrane was then probed with the following primary antibodies diluted in 5 % BSA /PBS-T for overnight at 4 °C: β-actin (1:1000; Cell Signaling Technology), ZO-1 (1.25 μg/ml) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... cells were blocked for 1h with 5% BSA in PBS and then stained with rabbit anti-pPLCγ1 antibody (Cell Signaling, #2821, 1/100) overnight at 4C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
-
bioRxiv - Physiology 2019Quote: ... blocked with 5% non-fat powdered milk in TTBS solution and incubated with primary antibodies from Cell Signaling (1:1000; Danvers, MA, USA) against p-IGF1RβY1135 (3918) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Histological sections (5 mm) were stained for immunohistochemistry using a standard protocol with antibodies for LC3A/B (1:200; #12741S; Cell Signaling Technology, USA).
-
bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
-
bioRxiv - Bioengineering 2022Quote: ... The membranes were blocked with PBS with 0.1% Tween-20 (PBST) with 5% Blotto for one hour and then incubated with antibodies to phospho-p90RSK (Thr359/Ser363; #9344; Cell Signaling Technology, Inc.) and RSK1/RSK2/RSK3 (32D7 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitates with anti-lamin A/C antibody were analysed in parallel with 5% unbound supernatants by immunoblotting with anti-lamin A/C (Cell Signaling; Cat# #4777) or anti-His (Cell Signaling ...
-
bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
-
bioRxiv - Neuroscience 2023Quote: ... For live cell microscopy cells were untreated or treated with 5 μM Aβ fibrils (as described above) pre-incubated with an Aβ antibody (Cell Signaling Technologies, #15126) conjugated to an Alexa Fluor 555 secondary (Invitorogen) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
-
bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
-
bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
-
bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... fixed cells were washed twice in PBS with 0.5% BSA prior to incubation with 1:500 dilution of phospho-histone H3 antibody conjugated with Alexa Fluor 488 (Cell Signaling Techonology, USA) for 1 h in the dark at room temperature ...
-
bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
-
bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
-
bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
-
bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
-
bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
-
bioRxiv - Cancer Biology 2019Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
-
bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
-
bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 5% milk and incubated overnight at 4 °C in primary antibody (anti-PPARγ, Santa Cruz #sc-7273; or anti-β-actin, Cell Signaling #3700). Washes were performed in TBS-T (20 mM Tris ...
-
bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
-
bioRxiv - Genetics 2023Quote: ... 0.1% Tween 20 and 5% BSA overnight at 4 °C with anti-Runx2 antibody (RUNX2 D1H7 Rabbit mAb, Cell Signaling Technology, 84866 1:2000) and anti-β-actin antibody (Rabbit Anti-beta Actin ...
-
bioRxiv - Cancer Biology 2023Quote: ... blocked in Tris-buffered saline/0.1% Tween (TBST)/5% milk and probed with antibodies against E-Cadherin (Cell Signaling Technology, 3195S, 1:1000 dilution), Vimentin (Cell Signaling Technology ...
-
bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
-
bioRxiv - Immunology 2023Quote: ... and incubated overnight at 4°C with mild agitation in 5% milk in TBST plus indicated antibody: cytochrome c (1:750, rabbit, Cell Signaling Technology, Cat. 11940), GAPDH (1:10000 ...
-
bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution, or rabbit anti-pMET, #3077, Cell Signaling Technology, 1:1000 dilution ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-pSMAD1/5 (Cell Signaling), anti-SMAD4 (Invitrogen ...
-
bioRxiv - Pathology 2022Quote: ... (Cell Signaling 15019S, 1:5) and then with diluted anti-αSMA-Cy3 antibody (Sigma Aldrich ...
-
bioRxiv - Pathology 2020Quote: ... Rab 5 (Cell Signaling, #3547S) and N-Cadherin (BD Transduction Labs ...
-
bioRxiv - Biochemistry 2024Quote: ... 5-LO (Cell signaling: 3289S), 12/15-LO (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... 5% BSA (Cell Signaling Technology) and primary antibodies were incubated in PBS-0.1% Triton X-100 ...
-
bioRxiv - Microbiology 2021Quote: ... Rabbit monoclonal anti-phospho-TANK Binding Kinase 1 (TBK1) (#5483) and anti-TBK1 (#3013) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal antibody against NDV nucleoprotein (NP ...
-
bioRxiv - Developmental Biology 2021Quote: ... Whole embryos were fixed in 4% paraformaldehyde overnight and stained with anti-phospho-Smad1/5/9 Rabbit monoclonal antibody (Cell Signaling Technology #13820S; 1:500), anti-phospho-H3 Rabbit monoclonal antibody (Cell Signaling Technology #9701S ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were then washed three times for 5 minutes each with 1X TBST and incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling, Cat # 7074S, 1:2000) in 5% BSA for two hours at room temperature with gentle rocking ...
-
bioRxiv - Neuroscience 2021Quote: ... The membranes were then blocked with 5% nonfat milk at room temperature for 1 hour and then probed with primary antibodies (phospho-p44/42 MAPK, Cell Signaling 9101, 1:4000, dilution) at 4°C overnight ...