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201 - 250 of 10000+ citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020

• Individual G-quadruplex targeting with ligand-functionalized CRISPR-Cas9 uncovers transcriptional-dependent functional responses

  Sabrina Pia Nuccio, et al., bioRxiv - Cell Biology 2024

  Quote: ... The membrane was blocked (5% milk in TBS-T) and incubated with primary antibodies (1:1000 Cas9 antibody-14697T, 1:1000 GAPDH antibody-2118T, Cell Signaling Technology) overnight at 4 degrees ...
• Second generation of LNP based mRNA vaccine leads to a T cell–inflamed tumor microenvironment favorable for improving PD-1/PD-L1 blocking therapy and long-term immunity in a cold tumor model

  Carole Fournier, et al., bioRxiv - Immunology 2024

  Quote: ... and then the slides were incubated with CD8 antibody (Histosure, polyclonal, 1/100, 30 minutes), CD4 antibody (Histosure, clone Rb78H9D2, 1/900, 30 minutes) or PD1 antibody (Cell Signaling, clone D7D5W, 1/50, 60 minutes). Slides were washed and then incubated with secondary HRP polymer reagents (Vector Laboratories ...
• Computationally designed GPCR quaternary structures bias signaling pathway activation

  J. S. Paradis, et al., bioRxiv - Biophysics 2022

  Quote: ... was added for 45 min then replaced by PBS + 1% BSA containing HA antibody 1:1000 (12CA5, Santacruz) or Myc antibody 1:1000 (D84C12, Cell Signaling) for 45 min ...
• The CD28-transmembrane domain mediates chimeric antigen receptor heterodimerization with CD28

  Yannick D. Muller, et al., bioRxiv - Bioengineering 2020

  Quote: ... anti Myc FITC or APC (clone 9B11, Cell Signaling, Danvers, MA), anti-FMC19 idiotype APC (Juno therapeutics) ...
• Intracellular Staphylococcus aureus perturbs the host cell Ca²⁺-homeostasis to promote cell death

  Kathrin Stelzner, et al., bioRxiv - Microbiology 2020

  Quote: ... Antibodies against Calpain 1 (1:1000, #2556, Cell signaling Technology), Calpain 4 (1:500 ...
• Ankyrin-B is lipid-modified by S-palmitoylation to promote dendritic membrane scaffolding of voltage-gated sodium channel Nav1.2 in neurons

  Julie P. Gupta, Paul M. Jenkins, bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-Flotillin-1 antibody (Cell Signaling Technologies, 1:1000), rabbit/mouse anti-HA antibody (Cell Signaling Technologies ...
• 3D synthetic microscaffolds promote homogenous expression of NANOG in mouse embryonic stem cells

  Natalia A. Bakhtina, et al., bioRxiv - Bioengineering 2020

  Quote: ... The following antibodies were used NANOG antibody (D2A3, Cell Signaling, 1:500) and OCT4 antibody (611202 ...
• Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

  Jinqiang Zhang, et al., bioRxiv - Neuroscience 2020

  Quote: ... Primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and the secondary antibodies (goat anti-rabbit IgG ...
• In the murine and bovine maternal mammary gland signal transducer and activator of transcription 3 is activated in clusters of epithelial cells around the day of birth

  Laura J. A. Hardwick, et al., bioRxiv - Cell Biology 2023

  Quote: ... Antibody for pSTAT3 (1:100, rabbit monoclonal antibody #9145, Cell Signaling Technology) was incubated overnight at 4°C and secondary antibody was incubated for one hour at room temperature ...
• Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy

  Zian Liao, et al., bioRxiv - Molecular Biology 2024

  Quote: ... PA antibody (Fuji Film, NZ-1) and PR antibody (Cell Signaling, D8Q2J) were added to each group respectively ...
• Pharmacologically modified pluripotent stem cell-based cancer vaccines with anti-metastatic potential

  Masae Heront-Kishi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... with or without the addition of the cytokines TNF-alpha (20 ng/mL, Cell Signaling Technology) and TGF-beta 1 (10 ng/mL ...
• Inactivation of the Hippo Tumor Suppressor Pathway Promotes Melanoma

  Marc A. Vittoria, et al., bioRxiv - Cancer Biology 2021

  Quote: ... secondary antibodies (1:5000, Cell Signaling Technology) for 1 h ...
• In-frame deletion of SPECC1L microtubule binding domain results in embryonic tissue movement and fusion defects

  Jeremy P. Goering, et al., bioRxiv - Developmental Biology 2021

  Quote: ... secondary antibody (1:10000; Cell Signaling Technologies) for 1 hour at room temperature ...
• SPEN is Required for Xist Upregulation during Initiation of X Chromosome Inactivation

  Teresa Robert-Finestra, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 1 μg of antibody (Cell Signaling, 9733S) was conjugated with 25 μL magnetic beads (Life Technologies ...
• GSAP regulates mitochondrial function through the Mitochondria-associated ER membrane in the pathogenesis of Alzheimer’s disease

  Peng Xu, et al., bioRxiv - Neuroscience 2020

  Quote: ... HMGCS1 antibody (1:1000, 42201S, Cell Signaling), SREBP2 antibody (1:1000 ...
• Reduced Shmt2 expression impairs mitochondrial folate accumulation and respiration, and leads to uracil accumulation in mouse mitochondrial DNA

  Joanna L. Fiddler, et al., bioRxiv - Biochemistry 2021

  Quote: ... Antibodies against SHMT2 (Cell Signaling, 1:1000), VDAC (Cell Signaling ...
• Live-cell imaging shows uneven segregation of extrachromosomal DNA elements and transcriptionally active extrachromosomal DNA clusters in cancer

  Eunhee Yi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... EGFR antibody (#4267, Cell signaling, 1:100) diluted in antibody diluent (1% BSA/0.3% Triton X-100/1X PBS ...
• Impairments in SHMT2 expression or cellular folate availability reduce oxidative phosphorylation and pyruvate kinase activity

  Joanna L. Fiddler, et al., bioRxiv - Biochemistry 2022

  Quote: ... Antibodies against SHMT2 (Cell Signaling, 1:1000), PKM1 (Cell Signaling ...
• Latent Plasticity of Effector-like Exhausted CD8 T cells contributes to memory responses

  Saravanan Raju, et al., bioRxiv - Immunology 2020

  Quote: ... Anti-TCF-1 antibody (Cell Signaling, C63D9) and detected by Alexa 488-conjugated Donkey anti-rabbit polyclonal IgG (Thermo Fisher ...
• HES1 is a Critical Mediator of the SHH-GLI3 Axis in Regulating Digit Number

  Deepika Sharma, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Antibodies against HES1 (1:1000; Cell Signaling), GLI3 (1:1000 ...
• Wild-type MECP2 expression coincides with age-dependent sensory phenotypes in a female mouse model for Rett syndrome

  Michael Mykins, et al., bioRxiv - Neuroscience 2022

  Quote: ... MECP2 primary antibody (1:1,000; Cell Signaling), and PV antibody (1:1,000 ...
• _Metabolic shift to serine pathway induced by lipids confers oncogenic properties in non-transformed breast cells

  Mariana Bustamante Eduardo, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 1:50 H3K27me3 antibody (Cell Signaling, #9733) and ...
• Early Deficits in Dentate Circuit and Behavioral Pattern Separation after Concussive Brain Injury

  Lucas Corrubia, et al., bioRxiv - Neuroscience 2023

  Quote: ... or PV antibody (1:500, Cell Signaling D3S8R ...
• A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation

  Katherine J. Susa, et al., bioRxiv - Immunology 2023

  Quote: ... SWAP70 antibody (Cell Signaling, 1:1000 dilution) or GAPDH-HRP Conjugate (Cell Signaling ...
• Phosphorylation and Lamin B receptor modulate the accumulation of PERIOD protein foci

  Mengna Li, Shujing Li, Luoying Zhang, bioRxiv - Cell Biology 2023

  Quote: ... HA antibody (1:50, Cell Signaling Technology) was added to SureBeads and incubated at 4°C for 4 hours ...
• Activity-dependent subcellular compartmentalization of dendritic mitochondria structure in CA1 pyramidal neurons

  Daniel M. Virga, et al., bioRxiv - Cell Biology 2023

  Quote: Antibodies: AMPKα (Cell Signaling, #2532S, 1:2000); phospho-AMPKα (Cell Signaling ...
• A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation

  Katherine J. Susa, et al., bioRxiv - Immunology 2023

  Quote: ... EAAT3 antibody (Cell Signaling, 1:1000 dilution), SWAP70 antibody (Cell Signaling ...
• Dynamic intestinal stem cell plasticity and lineage remodeling by a nutritional environment relevant to human risk for tumorigenesis

  Jiahn Choi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... F4/80 antibody (1:200, Cell Signaling, Denvers ...
• KSR1 knockout mouse model demonstrates MAPK pathway’s key role in cisplatin- and noise-induced hearing loss

  Matthew A. Ingersoll, et al., bioRxiv - Neuroscience 2023

  Quote: ... pERK antibody (1:400; 9101L, Cell Signaling), or anti-Tuj1 (1:400 ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... LC3B Antibody ((Cell Signaling Technology, 1:1000), Ubiquitin (P4D1 ...
• PSGL-1 excludes HIV Env from virion surface through spatial hindrance involving structural folding of the decameric repeats (DR)

  Sameer Tiwari, et al., bioRxiv - Microbiology 2024

  Quote: ... HRP-linked Antibody (1:1000) (Cell Signaling) was used as the secondary antibody ...
• Alteration of skin fibroblast steady state contributes to healing outcomes

  Yaqing Huang, et al., bioRxiv - Pathology 2024

  Quote: ... β-Catenin Antibody (1:500, Cell Signaling, 9562S), Anti-LOX antibody (1:500 ...
• CRISPRa screen on a genetic risk locus shared by multiple autoimmune diseases identifies a dysfunctional enhancer that affects IRF8 expression through cooperative lncRNA and DNA methylation machinery

  Tian Zhou, et al., bioRxiv - Genetics 2021

  Quote: ... or anti-PU.1 antibody (2266S, Cell Signaling Technology, 1:50) overnight at 4 °C on rotation ...
• Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondria

  Thorsten Neuß, et al., bioRxiv - Cancer Biology 2022

  Quote: ... with primary antibodies against Hexokinase 1 (1:500, Cell signaling, 2024), LDHA (1:10.000 ...
• Porphyromonas gingivalis activates Heat-Shock-Protein 27 to drive a LC3C-specific probacterial form of select autophagy that is redox sensitive for intracellular bacterial survival in human gingival mucosa

  Bridgette Wellslager, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-Beclin 1 rabbit antibody (Cell Signaling Technology, 3495; 1:1000), and anti-ATG14 rabbit antibody (Cell Signaling Technology ...
• The bacterial virulence factors rhamnolipids and their (R)-3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms

  Romain Schellenberger, et al., bioRxiv - Plant Biology 2021

  Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
• Cell type-selective secretome profiling in vivo

  Wei Wei, et al., bioRxiv - Biochemistry 2020

  Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194, 1:100 dilution; rabbit anti-V5 antibody, Cell Signaling Technology, 1:500 dilution). Sections were washed ...
• PINK1 and BNIP3 mitophagy inducers have an antagonistic effect on Rbf1-induced apoptosis in Drosophila

  Mélanie Fages, et al., bioRxiv - Cell Biology 2023

  Quote: ... First antibody incubation was made overnight using Dcp-1 antibody (Cell Signaling 95785S) diluted 1:100 in the same solution ...
• CDC20 determines the sensitivity to spindle assembly checkpoint (SAC) inhibitors

  Siqi Zheng, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Used secondary antibodies were anti-mouse Alexa-555 antibody (1:400, Cell Signaling) and an anti-rabbit Alexa-488 (1:400 ...
• Differential expression of GABARAPs in GBM renders temozolomide sensitivity in a p53-dependent manner

  Megha Chaudhary, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The antibody used was: Rabbit-monoclonal antibody LC3A (1:1000, D50G8, Cell Signaling), rabbit monoclonal antibody LC3A/B (1:1000 ...
• Diptera-specific Daedalus controls Zucchini endonucleolysis in piRNA biogenesis independent of exonucleases

  Yuica Koga, et al., bioRxiv - Biochemistry 2024

  Quote: ... the membranes were incubated with peroxidase-conjugated anti-mouse IgG antibodies (1:5,000 dilution, 55558, Cappel) or anti-rabbit IgG antibodies (1:5,000 dilution, 7074, Cell Signaling Technology). The membranes were then reacted with Clarity Western ECL Substrate (Bio-Rad ...
• Nanchangmycin regulates FYN, FAK and ERK to control the fibrotic activity of hepatic stellate cells

  Wenyang Li, et al., bioRxiv - Cell Biology 2021

  Quote: ... The following antibodies were used: FYN antibody (1:1000, Cell Signaling Technology, cat# 4023), phospho-Src family antibody (1:1000 ...
• Dynamic proteomics profiling of Legionella pneumophila infection unveils modulation of the host mitochondrial stress response pathway

  Julia Noack, et al., bioRxiv - Cell Biology 2020

  Quote: ... The following antibody dilutions were used: primary antibodies: ATF3 1:100 (Cell Signaling Technology), L.p ...
• Peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved

  Tamara Somborac, et al., bioRxiv - Cell Biology 2023

  Quote: ... Secondary antibodies: anti-mouse IgG HRP-linked antibody 1:5000 (Cell Signaling Technology, 7076). The chemiluminescence was developed using ECL substrate for enhanced chemiluminescence (Thermo Scientific ...
• Preclinical model for the study of immune responses specific for a hepatic self-antigen

  Anaïs Cardon, et al., bioRxiv - Immunology 2022

  Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
• Differential RNA editing between epithelial and mesenchymal tumors impacts mRNA abundance in immune response pathways

  Tracey Chan, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Vimentin antibody (Cell Signaling Technology, 5741, 1:1000), NF90(ILF3 ...
• Integrative analysis allows a global and precise identification of functional miRNA target genes in mESCs

  Moritz Schäfer, et al., bioRxiv - Systems Biology 2021

  Quote: ... AGO2 antibody 1:1500 (Cell Signaling; Cat#2897S), TFAP4 antibody 1:1000 (ab223771 ...
• Integrative analysis allows a global and precise identification of functional miRNA target genes in mESCs

  Moritz Schäfer, et al., bioRxiv - Systems Biology 2021

  Quote: ... DROSHA antibody 1:2000 (Cell Signaling; Cat#D28B1), DICER antibody 1:2000 (Sigma Aldrich ...
• Integrative analysis allows a global and precise identification of functional miRNA target genes in mESCs

  Moritz Schäfer, et al., bioRxiv - Systems Biology 2021

  Quote: ... AGO1 antibody 1:1500 (Cell Signaling; Cat#5053), AGO2 antibody 1:1500 (Cell Signaling ...
• The Endoplasmic Reticulum pool of Bcl-xL dampens the Unfolded Protein Response through IP3R-dependent Calcium Release

  Lea Jabbour, et al., bioRxiv - Cell Biology 2021

  Quote: ... Bcl-xL antibody (Cell Signaling #2764; 1/200) was used to detect endogenous Bcl-xL ...