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Citations for Cell Signaling Technology :
201 - 250 of 10000+ citations for 4 4 2 Hydroxy ethyl piperazin 1 ylmethyl benzoic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and then incubated overnight at 4℃ with rabbit anti-c-Fos antibody (1:1000 dilution, #2250, Cell Signaling Technology), mouse anti-OT antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4 °C with primary antibodies: rabbit anti-HA (1:200, Cell Signaling Technology, 3724S), chicken anti-GFP (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were incubated with the following primary antibodies overnight at 4 °C: SOX2 (1:1000, D9B8N, Cell Signaling), OCT4 (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... Protein lysates were incubated overnight at 4°C with mouse α-Myc antibody (1:400, [Cell Signaling Technologies, #2276]) on a rotisserie tube rotator ...
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bioRxiv - Plant Biology 2022Quote: ... AtRsmD-4×Myc fusion proteins were detected using a mouse anti-Myc monoclonal antibody (1:10,000; Cell Signaling Technology).
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated overnight at 4°C with rabbit α-phospho-ERK 42/44 (1:500, Cell Signaling Technologies). Slides were next incubated with AlexaFluor-555 tagged donkey-α-rabbit secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... IPs were incubated rotating overnight at 4°C with either IgG Isotype control (1:300, Cell Signaling Technology, 2729) or anti-ERH (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... and probed overnight at 4°C with primary antibodies: anti-cMyc (Cell Signaling technology, catalog no. 2276, 1:1000), anti-HA (Cell Signaling technology ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated overnight at 4°C with primary antibodies: anti-phosphotyrosine antibody (1:10,000; 8959; Cell Signaling), anti-GLUT4 antibody (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... Then organoids were incubated overnight at 4°C in 1:400 anti-Cleaved caspase 3 (CC3; Cell Signaling; 9661S). Organoids were rinsed 3 x 2 hours in OWB on a rocking platform before incubating overnight at 4°C in 1:250 donkey anti-rabbit Alexa Fluor 555 (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were incubated overnight at 4°C in primary antibody: F4/80(1:150, Cell Signaling, Cat. No.:70076), slides were incubated in secondary antibody for 1hr at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated with primary antibody rabbit-anti-FES (85704, Cell Signaling Technologies, 1:1000, o/n, 4 °C) in 5% (w/v ...
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bioRxiv - Pathology 2021Quote: ... and incubated with primary antibodies at 4°C overnight at the following concentrations: β-catenin (Cell Signaling, 8480, 1:1000 in 1% BSA), p65 (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatants were transferred to fresh 2 ml tubes and incubated ON at 4°C with either 5 µl α-H2AK119ub1 (D27C4, Cell Signaling Technology #8240), 15 µl α-H3K27me3 (C36B11 ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Immunology 2021Quote: Lungs were inflated with 4% PFA (Cell Signaling, San Diego, US) and fixed for four hours on a lateral shaker at 4°C ...
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bioRxiv - Immunology 2020Quote: ... anti-Caspase-4 (4450 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-5 (D3G4W from Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng/mL IL-4 (Cell Signaling Technology, USA, Cat: 5208SC) and 20 ng/mL GM-CSF (PeproTech ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 4 µM CHIR-99021 (Cell Signaling Technologies) in RPMI Glutamax (Thermofisher 61870127 ...
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bioRxiv - Genetics 2024Quote: ... incubation was overnight at 4°C and secondary antibody (Cell Signaling Alexa Fluor 594 anti-mouse 8890S ...
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bioRxiv - Cancer Biology 2022Quote: ... Tri-methyl-Histone H3 (Lys9) (D4W1U) (13969T, Cell Signaling Technology), Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Immunology 2024Quote: ... anti-tri-methyl-histone H3 (Lys27) (C36B11, Cell Signaling Technology), anti-mouse TNF-a (MP6-XT22 ...
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bioRxiv - Developmental Biology 2024Quote: ... Asymmetric Di-Methyl Arginine (ADMA) (Cell Signaling Technology Cat# 13522) to detect methyl-SFPQ ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Genomics 2023Quote: ... membranes were incubated overnight at 4°C with different primary antibodies in TBS-T against LEF-1 (1:500, Antibody# 2230, Cell Signaling Technology, MA, USA), and β-actin (1:30000 ...
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bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...
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bioRxiv - Physiology 2023Quote: ... Neurons were then incubated overnight at 4°C in 1% bovine serum albumin in PBS with pCREB (Ser133) (87G3) Rabbit mAb (1:333, Cell Signaling, Cat No. #9198). Neurons were then thrice rinsed with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 200 μl of 1XDPBS + 1% BSA + 0.1% Tween-20 containing 4 μl of Alexa Fluor 488-conjugated phospho-histone H3 (Ser10) antibody (Cell Signaling Technologies, 1:50 dilution) for 90 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual sections were then incubated overnight at 4 °C with a mixture of Tcf7 (1:200; C63D9, Cell Signaling Technology) and Cd200 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Twenty-five micrograms of each sample were incubated overnight at 4°C with pAMPKα2 (Cell Signaling®, USA, 1:1000) and AMPKα2 (T172 ...
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bioRxiv - Neuroscience 2020Quote: ... blots were probed with primary antibodies at 4°C overnight: rabbit anti-phsopho-S6240/244 (Cell Signaling, #5364, 1:4000), mouse anti-RPS6 (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... the tissues were stained 16 hours at 4°C with primary antibodies against γH2AX (1:200, 20E3, Cell Signaling Technology) diluted in the blocking medium ...
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bioRxiv - Immunology 2022Quote: ... and incubated at 4°C over night with a phospho-histone H2AX (pSer139) antibody (Cell Signaling Technology, #2577, 1:50). After washing with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated over sequential nights at 4°C in primary antibodies (Cell Signaling Technology 4370 and 4696; 1:500) and secondary antibodies conjugated with Alexa fluorophores (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 24 h at 4 °C with anti-Fos antibody (1:8000 dilution; Cell Signaling Technology, catalog #2250) in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated (overnight, 4°C) with monoclonal anti-HA antibody (rabbit, dilution 1:500, cat. 3724S; Cell Signaling), or anti-tubulin (rat ...