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Citations for Cell Signaling Technology :
2401 - 2450 of 3369 citations for Mouse Lactase Like Protein LCTL ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and horseradish peroxidase (HRP)-labeled anti-mouse IgG (H + L) (cat #, 7076S, 1: 2,000, Cell Signaling Technology, Danvers, MA, USA) were used as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-ý-Actin (Millipore Sigma, mouse mAb, 1:5000 or Cell Signaling Technology, rabbit mAb, 1:5000, as indicated). The secondary antibodies used were anti-rabbit HRP-linked (CST 7074 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was then incubated for 1 hour at room temperature with either DyLight 800 anti-mouse (Cell Signaling, #5257) or DyLight 580 anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal GAPDH (Thermo ScientificTM, Cat: MA5-15738) and rabbit α-TUBULIN (Cell Signaling Technologies™, Cat: 50-190-297) were used as loading controls in immunoblot assays.
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bioRxiv - Biophysics 2023Quote: ... The membrane was washed 3 times for 5 min in TBST (20 mM Tris pH 7.6, 150 mM NaCl, 0.1% Tween20) before incubation with anti-mouse-HRP (Cell Signaling Technologies) at a 1:2000 dilution in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected by incubating blots for 1h in secondary anti-mouse HRP conjugate diluted 1:6,000 or secondary anti-rabbit HRP conjugate (Cell signaling) diluted in milk blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated with Horse Raddish Peroxidase (HRP)-conjugated goat anti-mouse IgG secondary antibody (Cell Signaling, cat. #7076) at 1:20000 (to detect GluN2C or GluN2A ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with Alexa Fluor 488 or Alexa Fluor 555 tagged anti-mouse antibody (1:1000, Cell Signaling Technologies) for 1hr at RT in a humid black chamber ...
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bioRxiv - Neuroscience 2023Quote: ... and goat anti-mouse IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 488 (Cell Signaling Technology, #4408). Each well ...
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bioRxiv - Genetics 2023Quote: ... Brain sections were incubated with primary antibodies overnight at 4°C (mouse-NeuN, 1:500, abcam ab177487; rabbit-Cas9, 1:1,000, Cell Signaling clone D8Y4K ...
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bioRxiv - Pathology 2023Quote: ... Mouse lung tissue sections were stained with anti-CD31 antibody (77699, Cell Signaling Technology, Danvers, MA, USA 1:200 dilution), anti-TrkB antibody (AF1494 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... HRP-linked rabbit IgG (cat#: 7074) and HRP-linked mouse IgG (cat#: 7076) antibodies were purchased from Cell Signaling Technology. The anti-FLAG HRP antibody (clone M2 ...
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bioRxiv - Neuroscience 2023Quote: Cryosections of P0 mouse brains were immunolabeled with cleaved-caspase 3 (c-cas 3) antibody (Cell Signaling #9961, 1:4000) for apoptosis analysis ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min three times and then incubated with the appropriate secondary antibody (anti-mouse #7076 or anti-rabbit #7074 (both 1:1,000; Cell Signaling) or anti-goat (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were incubated with 0.5 ml PBS BSA 3% (A1391, Applichem) with primary antibodies (mouse-anti-HA monoclonal antibodies (C29F4, Cell Signaling) at 1:500 ...
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated in 4% milk supplemented with either anti-mouse IgG HRP-conjugated secondary antibody (Cell Signaling Technologies, 7076P2) (1:10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with peroxidase-conjugated anti-mouse IgG antibodies (1:5,000 dilution, 55558, Cappel) or anti-rabbit IgG antibodies (1:5,000 dilution, 7074, Cell Signaling Technology). The membranes were then reacted with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed with TBS-T and incubated at RT for 2 h with horseradish peroxidase-labeled secondary antibody (1:3,000, anti- rabbit #7074, anti-mouse #7076 or anti-rat #7077, Cell Signaling) diluted in a solution of 5% milk in TBS-T ...
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bioRxiv - Microbiology 2024Quote: ... Blots were then incubated with anti-rabbit IgG or anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibodies (1:10,000; Cell Signaling) in TBST-milk for 1-2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg total protein was resolved in 8% non-reducing SDS-PAGE gel and blot with PKM2 antibody (Cell Signaling, 3198s).
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked with Q-PBS and probed with antibodies against the following proteins: p-EGFR Y1068 (#3777S, 1:200, Cell Signaling), MET (#AF276 ...
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bioRxiv - Genetics 2021Quote: ... the samples were incubated with 30 ml of ChIP Grade Protein G magnetic beads (Cat# 9006S; Cell Signaling Technology; Danvers, MA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Plant Biology 2022Quote: ... The prey and bait proteins were detected using anti-His (1:1000, No. #2365; Cell Signaling Technology [CST], Danvers, MA, USA) and anti-GST (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of Anti-HA fusion protein was examined by staining cells with rabbit Anti-HA (1:250; #3724, Cell Signaling Technology) and Alexa Fluor@ 488 donkey anti-rabbit IgG (1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ATM protein expression was assessed by SDS-PAGE and western blot using a rabbit anti-ATM antibody (1:1000; 2873S; Cell Signaling). Horseradish peroxidase-conjugated α-rabbit antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting phosphorylated RB protein was detected by western blotting using anti-p-Rb (S807/S811) antibody (Cell Signaling Technology, 8516S).
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bioRxiv - Cell Biology 2021Quote: ... Protein accumulation rate upon proteasome inhibition was measured after 3-hour treatment with 10μM of MG-132 (Cell Signaling Technology #2194) in the RPMI medium.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of relative densitometry was obtained by normalizing to the background and to loading control proteins (ACTB, from Cell Signaling Technology) using Image Lab Software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Molecular Biology 2020Quote: Western blot was performed after chemoenzymatic labeling to detect O-GlcNAcylated proteins using α-streptavidin-HRP (Cell Signaling Technologies, Danvers, MA) (1:3000) ...
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bioRxiv - Microbiology 2020Quote: ... Lysed samples were centrifuged and incubated for 3 h with Pierce Protein A/G Magnetic beads (88802) which were pre-incubated over night with V5 antibody (Cell signaling E9H80 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Individual ChIP experiments were performed on pre-cleared chromatin using antibody-coupled ChIP grade Protein G magnetic beads (Cell Signaling Technology). POLR3A antibody was obtained from Abcam (ab96328 lot#GR318563) ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged and myc-tagged proteins were detected with rabbit anti-DYKDDDDK (FLAG) monoclonal antibody and rabbit anti-myc monoclonal antibody (Cell signaling), respectively ...
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bioRxiv - Neuroscience 2021Quote: Antibodies for detection of cell surface proteins by western blot of cell lysates were used as provided and were from the following sources: anti-ErbB3 (Cell Signaling, HER3/ErbB3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein levels were detected using antibodies against Intracellular Domain of Notch1 (ICN) (Val1744 Cell Signaling Technology #4147, D3B8, 1:500 dilution) and β-actin (Millipore-Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins on the gel were transferred onto PVDF membrane and were probed for specific antibodies against Rb (cat# 9309; Cell signaling) phospho-Rb (cat# 8516 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2α or total eIF2α (both antibodies were obtained from Cell Signaling). Expression of E was detected using an HA-tag antibody (Invitrogen) ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Human CDH5 and ICAM-1 protein levels were evaluated by SDS PAGE using rabbit anti-CDH5 (Cell signaling technology Cat# 2500) and mouse anti-ICAM-1 (Santa Cruz biotechnology ...