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Citations for Cell Signaling Technology :
2251 - 2300 of 2891 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... and immunoblotted for ubiquitin as per standard protocols (anti-ubiquitin antibody P4G7, BioLegend #838701, 1:1000; anti-mouse IgG HRP-linked antibody, Cell Signaling Technology #7076 ...
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bioRxiv - Immunology 2020Quote: ... Blots were then incubated in HRP-linked secondary antibody against mouse or rabbit IgG (1:3000, Cell Signaling, Cat# 7074S and 7076S) in 4% BSA for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoblots were then incubated with secondary antibody for 1 h at room temperature (1:1000 dilution, anti-rabbit Cat. #7074, lot 28, or anti-mouse HRP-conjugate Cat. #7076S, lot 32, Cell Signaling Technology). Specific signal was detected using SuperSignal West Pico Chemiluminescent Substrate (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed with Tween-TBS and incubated in secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076; both Cell Signaling technology) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... primary antibody pairs against NFκB total (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse-anti-NFκB p65 total, Cell Signaling Technology #6956); NFκB phospho (rabbit-anti-NFκB p65 total ...
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bioRxiv - Immunology 2022Quote: ... The following horseradish peroxidase (HRP) conjugated secondary antibodies were used for this study: mouse anti-rabbit IgG (L27A9) monoclonal antibody (Cell Signaling #5127), goat anti-rabbit IgG polyclonal antibody (Cell Signaling #7074S) ...
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bioRxiv - Physiology 2023Quote: ... Bound primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse or -rabbit secondary antibodies (Cell Signaling Technology, Inc., Danvers, MA), and protein signals were visualized using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100uM glycine for 30 minutes at room temperature and labeled overnight at 4C with primary antibodies (rabbit anti-FOXP2 Abcam #16046 1:500, rat anti-Flag 1:500 Novus NBP1-06712, mouse anti-NeuN Ab 1:500, Cell Signaling #94403S). For secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Rabbit Secondary Antibody in 1:30000 dilution and Anti-Mouse Secondary Antibody (Cell Signaling Technology, 5151; Li-Cor, 926-68020, respectively) were used in 1:15000 dilution during Western Blotting experiments.
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for WB: Anti-mouse IgG HRP antibody (Cell Signaling Technology #7076S, 1:10000) and anti-rabbit IgG HRP (Cell Signaling Technology #7074S ...
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bioRxiv - Cell Biology 2024Quote: ... the blots were washed with TBS-T(x3) and incubated with a secondary peroxidaseLconjugated antirabbit or mouse IgG antibody (1:2000) (#7074 or #7076, Cell Signaling Technology) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and detected using Goat anti-mouse Alexa Flour 647 and astrocytes were labelled with GFAP Alexa Flour 555 (3656S, Cell Signaling Technology). Slides were mounted with Prolong Diamond (P36961 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibodies (1:500 HCS-1 mouse monoclonal, DSHB, University of Iowa; 1:400 anti-cleaved Caspase-3 rabbit polyclonal, Cell Signaling Technology). After overnight incubation in primary ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Plant Biology 2024Quote: ... After addition of a 1:5000 dilution of horseradish peroxidase-conjugated horse anti-mouse secondary antibodies (Catalog #7076; Cell Signaling Technology, USA), proteins were detected using an ECL kit (LI-COR Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed 3X with TBST for a total of 15 minutes and incubated with HRP conjugated secondary anti-mouse (7076S) or anti-rabbit (7074S) antibodies at a 1:5000 ratio (Cell Signaling Technology) for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP, 7076; Cell Signaling) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... GST-PpROP4 was detected with an anti-GST primary antibody (26H1) of mouse origin (Cell Signaling Technology, cat. 2624-100µl, 5000× dilution) and an anti-mouse-HRP (goat ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2, 1:1000, Cell Signaling; rabbit anti-AKT3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat. 901514; rabbit, Cell Signaling 3724S), and two PLA probes (anti-rabbit-PLUS ...
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bioRxiv - Biochemistry 2022Quote: ... The blots were subsequently incubated overnight at 4°C with primary antibodies against p53 (DO-7 mouse mAb, Cell Signaling, Cat # 48818) and β actin (13E5 rabbit mAb ...
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bioRxiv - Microbiology 2023Quote: ... with HRP-conjugated secondary antibodies (Goat Anti-Rabbit Immunoglobulins/HRP, Dako P0448; Horse Anti-Mouse IgG, HRP-linked, Cell Signaling Technology #7076). BCIP/NBT substrate was used to detect ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed in TBST and then incubated for 45 min at room temperature in blocking buffer containing either anti-rabbit or anti-mouse HRP-linked secondary antibody (Cell Signaling Technology). Membranes were washed in TBST and imaged on the ChemiDoc Imaging System using SuperSignal chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... followed by 1 h incubation in horseradish peroxidase–conjugated secondary antibody (anti-rabbit or anti-mouse, #7074 and #7076, Cell Signaling Technology) diluted in PBS-T 5% milk at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... over night at 4 °C with a dilution of 1:200 and subsequent incubation with a HRP-linked secondary anti-mouse antibody (Cell Signaling #7076) for 2h at RT with a 1:5000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Cell lysates were analysed by western blot with anti-Gli1 (L42B10) mouse monoclonal antibody (1:800, 2643, Cell Signaling Technology, RRID: AB_2294746) followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-α-tubulin DM1α conjugated to Alexa Fluor 488 (1:100, Cell Signaling Technologies 8058S), mouse anti-centrin clone 20H5 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding secondary antibodies conjugated with horseradish peroxidase (cat no: 7074 Rabbit, cat no: 7076 Mouse, cat no: 7077 Rat, Cell Signaling Technology) in combination with enhanced chemiluminescence reagent (cat no ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against mouse anti-HA clone 6E2 (Cell Signaling Technology) or rabbit anti-TgSKP1 UOK75 [19].
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bioRxiv - Biochemistry 2023Quote: ... The blots were then incubated with a peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody (1:2000) (# 7074 or #7076, respectively; Cell Signaling Technology). The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... the fixed cells were blocked in PBS containing 5% bovine serum albumin (BSA) and then incubated with mouse anti-HA antibody (6E2, Cell signaling Technology) with 1:800 dilution overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... neurons and mouse brain tissues were lysed for 30 minutes on ice in appropriate volume of cold RIPA lysis buffer (Cell Signaling Technologies) supplemented with protease inhibitor cocktail ...
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bioRxiv - Immunology 2023Quote: ... Rabbit monoclonal anti-FLAG (D6W5B) antibodies (Cat # 14793S) and Mouse monoclonal anti-MYC (9B11) antibodies (Cat# 2276S) were obtained from Cell Signaling Technology. Mouse polyclonal anti-V5 antibodies were purchased from MBL Life Science (Cat# PM003) ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies have been used on human PDAC tissue of figure 1b or mouse PDAC tissue from figure 2b: pan-Keratin (#4279, Cell Signaling Technology), p-ERK (#9101 ...
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bioRxiv - Cell Biology 2023Quote: ... Cat. 97166 [D4C6R], mouse mAb, 1:1000; Akt, Cell Signaling, Cat. 4685 [11E7], rabbit mAb, 1:1000; p-Akt [Ser473], Cell Signaling, Cat ...
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bioRxiv - Immunology 2023Quote: ... The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100, 78060S, Cell Signaling Technology) in Ce3D™ Antibody Diluent Buffer (48 hr ...
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bioRxiv - Cell Biology 2024Quote: ... At least 1 mg of proteins were immunoprecipitated with mouse anti-Ezrin (Novex, 357300) and rabbit anti-EGF receptor (Cell Signaling 4267) in rotation at 4° C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-linked goat anti-rabbit IgG (H+L) antibody (7074) and HRP-linked goat anti-mouse IgG (H+L) antibody (7076) were purchased from Cell Signaling Technology. Anti-mCherry antibody (SAB2702291) ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [HRP-conjugated anti-mouse IgG (1:5000; Cell Signaling Technology; #7076) and HRP-conjugated anti-rabbit IgG (1:5000 ...
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bioRxiv - Bioengineering 2024Quote: ... 45 µL of cells suspension were incubated for 90 min at RT in a total volume of 55 µL with 10 nM BioGCSFR and 1:200 Myc-Tag (9B11) Mouse mAb (2276, Cell Signaling Technology). After one wash with 500 µL PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were rinsed three times for 10 minutes and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse antibodies (1:2,500, Cell Signaling, #7074, #7076) at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...