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Citations for Cell Signaling Technology :
2251 - 2276 of 2276 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Detection was performed with the UVP ChemStudio Imaging System (Analytik Jena, Jena, Germany) using horseradish peroxidase-linked anti-mouse (#38) and anti-rabbit (#29) secondary antibodies (Cell Signaling Technology, Danvers, MA, USA), and the ECL western blotting reagent ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were treated with the following primary antibodies (αSMA, mouse, Cat. No. ab7817, Abcam at 1:300 dilution, PTEN rabbit, Cat. No. D8H1X, Cell Signaling at 1:300 dilution) sequentially over 2 days with 2 overnight incubations with the primary antibody in blocking buffer (dilution 1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Membranes were washed 3 x and incubated for 1 h at room temperature with the horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse-HRP, 1:2000; Cell Signaling Technology, Danvers, MA, USA), washed in LSWB and incubated with chemiluminescent HRP substrate reagent (Super Signal West Femto ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with secondary antibodies (goat anti-rabbit monoclonal IgG, HRP-linked, 1:1000, Cell Signaling Technology, #7074S; horse anti-mouse IgG monoclonal, HRP-linked, 1:2000, Cell Signaling Technology, #7076S) diluted in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-GM130 (BD transduction Laboratories, ref:610822, 1:1000, rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies at 1:10,000 (Mouse, Cell Signaling technology Cat# 7076, RRID AB_330924; Rabbit, Cell Signaling technology Cat# 7074, RRID AB_2099233) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated at 37°C for 2 h with the corresponding secondary antibody: horseradish peroxidase (HRP)-linked anti-mouse IgG antibody (#7076, Cell Signaling Technology [CST]; diluted 1:2000) or HRP-linked anti-rabbit IgG antibody (#7074 ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were then incubated at 4°C overnight in Odyssey Blocking buffer containing 0.1% Tween 20 and the following antibodies: ERK 1/2 (mouse monoclonal, Cell Signaling Technology no. 4696; 1:1000 dilution), pERK 1/2 (rabbit monoclonal ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated, Cell Signaling, Cat #: 7076, 1:5,000 dilution) prepared in the WB blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... NF-KB (1:1000 mouse anti-total NF-κB; Cell Signaling Technology #6956; RRID #AB_10828935 or 1:1000 rabbit anti-phospho NF-κB-S536; Cell Signaling Technology #3033; RRID #AB_331284); Akt (1:250 mouse anti-total Akt ...
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bioRxiv - Microbiology 2024Quote: ... blocked in 3% goat serum for 1 hour at room temperature and incubated in mouse anti-Sag1(28) [DG52] and anti-NF-κB (Cell Signaling Technology, 8242S, 1:1000 antibodies) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then reacted with a secondary antibody (Anti-mouse IgG, and Anti-rabbit IgG conjugated to Horseradish Peroxidase (both from Cell signaling, Cat # 7076 and # 7074, respectively), developed using Western BrightTM enhanced chemiluminescence reagent (Advansta) ...
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bioRxiv - Microbiology 2023Quote: ... Wells were washed three times and incubated with HRP-labeled anti-mouse IgG H+L (1:2500 dilution in blocking buffer) (Cell Signaling Technology, Danvers, MA, USA, #7076P2). Plates were developed with 1-Step ultra TMB-ELISA substrate solution and incubated at room temperature for 15-30 min ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed for 30 min with TBS-0.05% Tween 20 (TBS-T) and primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse secondary antibody diluted 1:2000 (Cell Signaling Technology, Inc., Danvers, MA, USA) and incubated at room temperature for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were analyzed by western blot using a mouse anti-HA antibody (Covance, Cat #MMS-101P; Sorcs1HA/HA KI) or a rabbit anti-HA antibody (Cell Signaling Technology, Cat #3724; Nrxn1αHA/HA KI). A total protein stain (Ponceau ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Then membranes were incubated for 1 h at RT with secondary anti-mouse or anti-rabbit HRP-linked antibodies (Cell Signaling Technology #7076 or # 7074; 1:5000) diluted in Bullet Blocking One and were washed three times for 15 min in 1×TBST buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were blotted on Nitrocellulose membrane (Cytiva) and revealed using following antibodies: mouse anti-AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000, rabbit anti-XBP1s (Cell Signaling Technologies, clone E9V3E, 1/1000), rabbit β-Tubulin-HRP (Abcam ...
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bioRxiv - Genetics 2023Quote: ... Alexa Fluor 488 anti-mouse IgG (H+L) (Catalog # 4408S) and Alexa Fluor 488 anti-rabbit IgG (H+L) from Cell Signaling Technology (1:200 dilution was used), CytoPainter phalloidin-iFluor 488 reagent (catalog # ab176753 ...
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bioRxiv - Biophysics 2023Quote: As primary antibodies we use mouse monoclonal anti-α-tubulin (dilution 1:250, Invitro-gen, #62204) and rabbit monoclonal anti-vimentin (D21H3) (dilution 1:200, Cell Signaling Technology, Danvers, MA, USA, #5741). As secondary antibodies we use goat anti-mouse Alexa Fluor 488 (dilution 1:500 ...
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bioRxiv - Physiology 2024Quote: ... or corresponding IgG controls (Mouse IgG2B Isotype Control MAB004 R&D Systems, Minneapolis, Minnesota, USA and rabbit IgG Isotype Control 3900S Cell Signaling Technology, Danvers, MA, USA). The following PLA steps ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S; anti-mouse-HRP or Cell Signaling Technology; 7074S; anti-rabbit-HRP) at 1:1000 in 2.5% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were then incubated with the appropriate secondary antibodies in TBS-T for 30 min at RT at 1:4000 dilution (Cell Signaling Technology, Anti-rabbit #7074, Anti-mouse #7076). Following washes with TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Microbiology 2019Quote: ... 1:10,000 dilution]) and then with secondary antibody (horseradish peroxidase–conjugated anti-rabbit IgG [Cell Signaling Technologies, 7074S] or anti-mouse IgG [Cell Signaling Technologies, 7076S], both at a 1:5,000 dilution]).