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Citations for Cell Signaling Technology :
2201 - 2250 of 2288 citations for Recombinant Mouse Enpp7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
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bioRxiv - Microbiology 2023Quote: ... expression of hACE and cACE2 was validated via immunofluorescence and immunoblots through detection of the c-terminal myc tag with a 1:500 dilution of antibody Myc-Tag-9B11 Mouse mAb (Cell Signaling Technology, 2276).
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling #7074; Anti-mouse IgG, HRP-linked Antibody, Cell Signaling #7076) and developed with a luminol-based enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBS-Tween three times for 10 min at RT and incubated with the secondary antibody anti-mouse-HRP (1:5000) (Cell Signaling, Danvers, MA) for one hour at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were diluted in 1% milk in TBST prior to usage at the indicated concentration: mouse monoclonal α-Tubulin (1:3000, Cell Signaling Technology, #3873), rabbit monoclonal p-p70 S6K (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were washed and incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:3000, Cell Signaling Technology, #7076 or #7074) and followed by detection with ECL Western blot substrate (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Cell Biology 2020Quote: ... A polyclonal antibody against phosphorylated Tyr416 of Src and a polyclonal antibody against mouse caspase-3 were from Cell Signaling Technology (Beverly, MA, USA). α-Thrombin was purchased from Enzyme Research Laboratories (South Bend ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen detection was performed by overnight incubation with appropriate antibodies: 1:1000 rabbit monoclonal Plexin-A4 (catalog #C5D1) anti-mouse (Cell Signaling Technology, catalog #3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6 ...
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bioRxiv - Genetics 2019Quote: ... Secondary antibodies (a donkey anti-goat IgG-HRP antibody, Santa Cruz Biotechnology, sc-2020, and a horse anti-mouse IgG-HRP antibody, Cell Signaling Technology, #7076), conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:1000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:2000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit anti-NF-κB2 p100/p52 (#4882), rabbit anti-RelB (C1E4, #4922), rabbit anti-c-Rel (D4Y6M, #12707), mouse anti-GAPDH (D4C6R, #97166) (Cell Signaling Technology, Danvers, MA); rabbit anti-H3(ab1791) ...
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bioRxiv - Plant Biology 2020Quote: ... s6k1 and tor to determine the cross-link between TOR and RPs using anti-human S6K1 antibody raised in mouse (anti phospho70kDa-S6K1-Thr(P)-389) (Cell Signaling Technologies, cat# 9206). The anti-70S6K1 (CST ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Cell Biology 2021Quote: ... and a mouse monoclonal antibody against the Ser19 phosphorylated form of the myosin II regulatory light chain (P-MyoRLC) from Cell Signaling Technology (Danvers, MA). Appropriate secondary antibodies conjugated to Alexa Fluor 488 ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Each membrane was washed and primary antibodies (except β-actin-HRP) were detected with a 1:5000 dilution of HRP-conjugated rabbit anti-mouse IgG (Cell Signaling Technology, 7076S) or mouse anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Genetics 2020Quote: ... Cells were filtered and incubated in a 1:500 solution of mouse anti-HA antibody covalently bound to Alexa 647 (Cell Signaling 6E2, product #3444). Cells were incubated with the antibody by shaking vigorously for 15-30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit monoclonal, 1:1000), haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000) or IκBα (mouse monoclonal, 1:1000; Cell Signaling Technology, Leiden, The Netherlands) in Tris-buffer saline solution containing 0.1% Tween-20 and 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibodies used were Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5151 and Anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5257 (Cell Signaling Technology) respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed three times for 5 min per time with shaking then incubated with secondary antibodies (anti-mouse IgG, HRP-linked antibody, Cell Signaling, Danvers, MA, USA) diluted in 5% w/v nonfat milk at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... blotted with mouse anti-ERK1/2 (p44/42 MAPK) and rabbit anti-phospho-ERK1/2 (p44/42 MAPK) (Cell Signaling Technologies 4696S and 4370S), both at 1:1000 in 2% BSA at room temperature for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated horse anti-mouse IgG solution (1:2500, Cell Signaling, Cat. No. 7074P2) diluted in 1% non-fat milk/TBST ...
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bioRxiv - Biophysics 2023Quote: ... and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat. 7076; anti-rabbit IgG, Cell Signaling, cat. 7074) diluted 1:5000 in 5% non-fat dried milk in TBS-T solution ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG at 1:5000 dilution or anti-mouse IgG at 1:5000 dilution; both from Cell Signaling Technology, Danvers, MA), and the bound proteins were visualized using a chemiluminescence-based detection kit (ImmunoStar Zeta ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:4700 in 5% non-fat milk (anti-rabbit IgG #ADI-SAB-300-J, Enzo Lifesciences, Farmingdale NY or anti-mouse IgG #7076, Cell Signaling Technology, Danvers MA). The membranes were imaged using Azure Biosystems Radiance Plus substrate solution and an Azure c600 Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... Anti-EGFR (D38B1) XP® Rabbit mAb #4267 and anti-EGFR Mouse mAb (IP Specific) #2256 were purchased from Cell Signaling Technology (Danvers, MA). The anti-β-actin antibody was purchased from Abcam (Cambridge ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% BSA diluted in TBS-Tween0,1% for 1 hour and primary antibodies were incubated overnight (rabbit anti-HER2 #29D8 Cell Signaling; mouse anti-vinculin #A250291). Donkey anti-mouse IgG or goat anti-rabbit IgG secondary antibody conjugated to horse-radish peroxidase (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of MBP-FoxP3ΔN was validated by Western blot using Mouse MBP Tag antibody(8G1) (Cell Signaling Technology, Cat#2396, 1:2000 dilution).
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bioRxiv - Neuroscience 2023Quote: ... was incubated overnight at 4°C followed by corresponding horseradish peroxidase (HRP)-linked anti-mouse (Cell Signaling Technology, 7076V, 1:2000 dilution in TBST) or anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... we incubated the cells with fluorescent secondary FAB fragments raised to the species corresponding to the primary antibody for 1 hr in PBS supplemented with 10% FBS (either Anti-rabbit IgG Fab2 Alexa Fluor ® 555 or Anti-mouse IgG Fab2 Alexa Flour ® 488, both Cell Signaling Technology, Catalogue # 647 4414 S and # 4408 S ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-MSP1 antibody (European Malaria Reagent Repository, 1:500), rat anti-PfBiP MRA-1247 (BEI Resources, NIAID, NIH, 1:100) and mouse anti-HA(6E2) (Cell Signaling Technology Inc., 1:100). Anti-mouse and anti-rat antibody conjugated to Alexa Fluor 488 or Alexa Fluor 546 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed three times for 5 min in TBS 0.1% Tween-20 and blots were probed with either HRP-conjugated anti-rabbit or anti-mouse secondary antibody (Cell Signaling, cat: 7074 and 7076, respectively). Membranes were then rinsed three times and treated with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in a solution of rabbit anti-c-fos primary antibody (1:1000 dilution, 2250T Rabbit mAb /74620 Mouse mAb, Cell Signaling Technology, Danvers, MA, USA) or mouse anti-TPH2 primary antibody (1:100 dilution ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times with PBS-T (PBS with 0.05% Tween-20) with automatic plate washer (250 μL per cycle) and 50 μL of HRP anti-mouse IgG (Cell Signaling Technology #7076, 1:3,000) or HRP anti-mouse IgA (Southern Biotech #1040-05 ...
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bioRxiv - Biochemistry 2019Quote: ... The following secondary antibodies were used: horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody and HRP-conjugated anti-rabbit IgG antibody (1:3000 dilution, Cell Signaling Technology, Beverly, Massachusetts, USA). HRP-dependent luminescence was developed with Western Lightning Chemiluminescent Reagent Plus (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in HRP-conjugated secondary antibodies (donkey anti mouse HRP, Jackson ImmunoResearch, Cat. #715-035-150 or Goat anti rabbit HRP: Cell Signaling Technology, Cat. # 7074S). Membranes probed for p62 were washed with 0.01% TBS-Triton X and incubated in biotinylated anti-guinea pig (goat polyclonal ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-KDM5 (1:1000, Secombe J; Genes Dev. 2007; Cat# lid, RRID: AB_2569502) and mouse anti-HA (1:1000, Cell Signaling Technology Cat# 2367, RRID: AB_10691311). The following secondary antibodies were used ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed 3 times for 10 minutes in TBS-T buffer and then incubated in secondary antibodies made against mouse and rabbit as appropriate in milk at a dilution of 1:4000 (Cell Signaling Technologies, Danvers, MA, USA). Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Neuroscience 2020Quote: ... The IP product was then run on a Western blot and probed using both the rabbit anti-mouse panAkt (1:1000) and Phosopho-Akt (Ser473, Cell Signaling Technologies 4060; 1:1000) antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated in secondary antibody (HRP conjugated anti-rabbit or anti-mouse IgG(H+L) (1:10,000 dilution, Cell Signaling, cat # 7074P2 or 32230, respectively). Subsequently the signal was developed using Super Signal West Femto substrate (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Mouse anti c-Myc mAb (clone 9B11, #2276) and mouse anti HA mAb (6E2) (catalogue number 2367) were obtained from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-CAV1 pAb (catalog number 610060) ...