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Citations for Cell Signaling Technology :
2201 - 2250 of 10000+ citations for Anti RASIP1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Membranes were washed with PBS-T and incubated with HRP-coupled goat anti-mouse or anti-rabbit IgG antibodies (Cell Signaling). Detection was performed with Clarity Lightning ECL (Bio Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... and antibody (1.2 ug of anti-H3K4me1: Diagenode, C15410194, Lot A1862D or 4 uL anti-H3K4me2: Cell Signaling #9725, Lot 9) was added per 0.6 mL-tube ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
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bioRxiv - Biochemistry 2022Quote: ... We incubated coverslips with primary antibodies diluted in goat serum (Anti-HA, Roche, ref #11867423001; Anti-Tom20, Cell Signaling, ref #42406S) in a humid chamber overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 2021) using 25μg of chromatin and 10ug of either anti-HIF1α ChIP antibody (Novus) or anti-rabbit IgG (Cell Signaling Technologies).Chromatin was immunoprecipitated overnight at 4C and HIF1α binding sites assayed via RT-qPCR using 2μl of either input ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (FosB anti-rabbit HRP, GAPDH anti-mouse HRP, Cell signaling) at 1:500 for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used to check the expression level of CBP and P300 by Western blot using anti-P300 and anti-CBP antibodies (Cell Signaling). Anti–β-tubulin antibody (Hybridoma bank # AB_2315513 ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody was either goat anti-rabbit (7074) or horse anti-mouse (7076) IgG conjugated with horseradish peroxidase (Cell Signaling Technology). Staining was developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were then incubated in the same buffer with anti-TAZ and anti-β -actin antibody (#8418, #5125, Cell Signaling) or anti-IHH antibody (#13388-1-AP ...
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bioRxiv - Biochemistry 2023Quote: ... anti-phospho-Akt (Thr308) (C31E5E) and anti-phospho-p44/42 MAPK (Erk 1/2) (Thr202/Tyr204) (E10) antibodies (Cell Signaling Technology). Each experiment was repeated 4 times ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in HRP-linked anti-mouse or anti-rabbit IgG antibody for 1h at 24°C with gentle rocking (1:5,000 dilution, Cell Signaling Technology). Membranes were washed in TBST and protein bands were visualized using the Pierce ECL detection system (Cat #32106 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for immunohistochemical studies included: anti-IGF-1R (#14534) and anti-cleaved-caspase 3 (#9661) from Cell Signaling Technology; and anti-CD99 (#ab-227738 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated with horse radish peroxidase-linked anti-mouse or anti-rabbit secondary immunoglobulin antibodies (1:10000; Cat# 7076S, Cat# 7074S Cell signaling) in PBST for 40 mins ...
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bioRxiv - Cell Biology 2023Quote: ... to the following proteins were used for the immunodetection of proteins using horseradish peroxidase-conjugated anti-mouse (Cytiva, NA931) and anti-rabbit (Cytiva, NA934) secondary antibodies (1:1,000): CHOP (Cell Signaling, 5554), Cleaved Caspase-3 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 1h with primary antibodies (1:4,000 dilution, anti-flag mouse -Sigma #F1804 or anti-myc mouse - Cell Signaling #2276). Membranes were then incubated for 1h with a secondary antibody (anti-mouse HRP conjugate at 1:10,000 dilution -Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... HA and Myc tag wer detected using anti-HA (see above) an anti-Myc monoclonal antibody (Cell Signaling Technology; Cat# 92013S) at 0.035µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with a respective secondary HRP-conjugated antibody (anti-rabbit: Cell Signaling Technologies, #7074S, anti-mouse: Cell Signaling Technologies, #7076) for one hour at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was incubated with primary antibodies (rabbit anti-EGFR D38B1 Cell Signaling Technologies, rabbit anti-HER2 2242 Cell Signaling Technologies, rabbit anti-PD-L1 E1L3N Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 5X with cold TTBS and incubated with anti-Rabbit or anti-mouse secondary antibodies conjugated to HRP (#7074 and #7076, Cell Signaling). After washing ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections post blocking were then incubated with primary antibody (rabbit anti-Phospho-S6, Invitrogen, Cat# 44-923G, 1:1000 dilution; rabbit anti Phosphp-Stat3, Cell Signaling, Cat# 9145 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The anti (pan)14-3-3 rabbit polyclonal antibody (#8312) and anti α-tubulin mouse monoclonal antibody (clone DM1A, mAb #3873) were purchased from Cell Signaling Technology. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2021Quote: ... The following primary and secondary antibodies were used in WB including anti-ICAM1 rabbit antibody (Cell Signaling Technology, Cat. No. 4915S, Danvers, USA), anti-RAB5C rabbit antibody (Thermo ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies against phospho-STAT1 and STAT1 and horseradish peroxidase-conjugated goat anti-rabbit antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA).
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bioRxiv - Cell Biology 2019Quote: ... blocked for an hour with 5% BSA-Tris Buffered Saline (TBS) and probed with primary antibodies anti-HA (C29F4) Rabbit monoclonal antibody (mAb) (Cell Signaling Technology®) or mouse raised anti-GFP antibodies (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-rat secondary antibody was used for detection of primary antibodies and visualized by SignalStain DAB Substrate kit (Cell Signaling Technology, Danvers, MA). All stained tissues were blindly evaluated by pathologists ...
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bioRxiv - Physiology 2024Quote: ... The rabbit monoclonal anti STAT1 phospho-Tyr701 antibody (clone 58D6, #9167) and the anti α-tubulin mouse monoclonal antibody (clone DM1A, mAb #3873) were purchased from Cell Signaling Technology. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Bioengineering 2023Quote: ... secondary antibodies were incubated for 1 hour at room temperature (Secondary antibodies: Anti-Rabbit HRP-conjugated Cell Signaling Technology (CST-#7074S 1:5000); Anti-Mouse HRP-conjugated (R&D ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoprecipitation was performed with 2 µg rabbit polyclonal anti-KDM2B (gift from Dr. Robert Klose, Oxford University) and rabbit anti-RING1B antibody (1:100, Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Antigen recognition was performed by incubating primary antibody in a humidified chamber for different times (Phalloidin, Invitrogen, A12381; anti-Vinculin, Invitrogen, 42H89L44; anti-pFAK, Cell signaling, 3283S) and with anti-mouse/rabbit Alexa Fluor 488-594 (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Biophysics 2022Quote: ... Cellular protein tyrosine phosphorylation was performed by immunoblotting of total cell lysate (20 μg) using anti-pTyr antibody (4G10, a gift form Prof. Mangeat; CRBM) and anti-Actin (Cell Signaling Technology). Signal quantification and analyses were performed using ImageJ and GraphPad Prism respectively ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti TRA-1-81 (1:200) and mouse anti-SSEA4 (1:200) (all from Cell Signaling Technology, StemLight Pluripotency Antibody Kit) to assess for pluripotency ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and horseradish peroxidase-conjugated (HRP) anti-mouse and anti-rabbit secondary antibodies were all obtained from Cell Signaling Technologies (Beverly, MA, USA). Mini-protean precast Tris-Glycine Gels (TGX ...
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bioRxiv - Developmental Biology 2021Quote: ... A mixture of rabbit anti-pSmad1/5/9 (D5B10) and anti-pSMAD1/5 (41D10) monoclonal antibodies (Cell Signaling, #13820 and #9516, respectively), diluted 1:100 each ...