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Citations for Cell Signaling Technology :
2151 - 2200 of 2294 citations for Recombinant Mouse ACVR2B Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 500 μL of supernatant were transferred to tubes containing 25 μL of prewashed magnetic α-Myc beads (Myc-tag [9B11] mouse mAb magnetic beads conjugated #5698; Cell Signaling Technology) and incubated at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... a mouse monoclonal antibody against the Ser19 phosphorylated form of myosin II regulatory light chain (P-MyoRLC) from Cell Signaling Technology (Danvers, MA), a rabbit monoclonal antibody against a peptide from human septin 2 from Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were horse anti-mouse HRP or goat anti-rabbit HRP which were both diluted 1:1,000 in block (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
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bioRxiv - Cell Biology 2021Quote: ... Washed membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies as indicated (1:2000, Cell Signaling Technology, Beverly, MA) in 10 ml of blocking buffer with gentle agitation for 1 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Individual clones were grown and stained for dCas9KRAB with a Cas9 antibody (Mouse mAb IgG1 clone 7A9-3A3 Alexa Fluor 647 Conjugate, Cell Signaling Technologies, 48796) to assess protein expression ...
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bioRxiv - Microbiology 2021Quote: ... and sera from individual animals were tested by ELISA with KLH-conjugated peptide immobilized on plastic surface and anti-mouse or anti-rabbit horse radish peroxidase (HRP)-conjugated detector Ab (Cell Signaling Technology, USA). The animals with the highest Ab titer were selected for booster immunization ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated for one hour with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Cell Signaling Technology; catalog #’s: 7076 and 7074). Membranes were then washed three times in TBST only (five minutes per wash) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times for 5 min with PBS and incubated in anti-mouse IgG Alexa Fluor® 555 Conjugate (Cell signaling #4409) at a dilution of 1:500 for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S ...
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bioRxiv - Microbiology 2022Quote: Rabbit anti-α-tubulin was purchased from Sigma (cat# SAB3501072) and goat anti-rabbit IgG HRP-conjugated and goat anti-mouse IgG HRP-conjugated were purchased from Cell Signaling (cat# 7076S).
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bioRxiv - Cancer Biology 2022Quote: ... and horseradish peroxidase (HRP)-conjugated rabbit and mouse secondary antibodies for immunoblot and MitoTracker Red CMXRos for immunofluorescence purchased from Cell Signaling Technologies (USA). Mouse monoclonal primary antibodies against p53 (DO-1) ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the goat anti-mouse (polyclonal 7076) and goat anti-rabbit (polyclonal 7074) peroxidase-conjugated secondary antibodies were from Cell Signaling (Beverly, MA. USA). DMEM complete medium ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody binding was directed against eGFP using a solution containing a 1/4000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, USA). For all VLPs ...
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bioRxiv - Developmental Biology 2022Quote: ... MilliporeSigma, Cat# V8012), Rabbit anti-HA antibody (1:1000, MilliporeSigma, Cat# H6908) and Mouse anti-Myc antibody (1:1000, Cell Signaling, Cat# 2276S) as primary antibody ...
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bioRxiv - Immunology 2019Quote: ... IL-1β mouse mAb (3A6) and monoclonal rabbit anti-Caspase-1 (catalog no. 2225) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054 ...
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bioRxiv - Genetics 2019Quote: ... and the primary antibodies were recognized by Alexa Fluor® 594 Donkey anti-rabbit IgG (H+L) (ANT030, Antgene) or Alexa Fluor® 555 Anti-mouse IgG (H+L) (4409S, Cell Signaling Technology) and shaken at room temperature for 1.5 hrs in the dark ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were incubated for 1 hour with 1:2000 dilution of either horseradish peroxidase (HRP)-linked horse anti-mouse IgG (Cell Signaling Technologies #7076) or HRP-linked goat anti-rabbit IgG (Cell Signaling Technologies #7074 ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100; Mouse Tubulin 1:1000; Cell Signaling DM1A). Primary incubation was followed with HRP-conjugated secondary antibodies (Rabbit-HRP 1:10000 ...
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bioRxiv - Immunology 2020Quote: ... IL-1β mouse mAb (3A6) and monoclonal rabbit anti-caspase-1 (catalog no. 2225) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054) ...
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bioRxiv - Molecular Biology 2019Quote: ... UK with catalogue numbers ab52908 and ab76227 respectively and housekeeping mouse raised β-tubulin (51 kDa) primary antibody was procured from Cell Signaling Technologies, MA ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were washed and incubated with either secondary goat anti-rabbit IgG or goat anti-mouse IgG at 1:5,000 dilution (Cell Signaling Technology, Danvers, MA) at room temperature for 90 min ...
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bioRxiv - Cell Biology 2019Quote: ... Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53, SC-126, Santa Cruz Biotechnology; rabbit -CTCF, Millipore 07-729; rabbit -GAPDH, cst-2118, Cell Signaling Technology) followed by detection with HRP conjugated secondary antibodies (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed twice with PBS and horse-anti-mouse-IgG-conjugated to HRP was added (1:2000 in 1% milk in PBS, Cell Signaling Technology, S7076) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Tween-20 in 1x TBS) and then treated with primary antibodies (YAP/TAZ, anti-rabbit, Cell Signaling, 8418 and beta-Actin, anti-mouse, Cell Signaling, 3700) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... β-actin [8H10D10] primary antibodies and anti-rabbit-HRP or anti-mouse-HRP antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). IRF3 [A19717] were purchased from ABclonal (Woburn ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... To confirm the specificity of staining cells were incubated with isotype mouse (7076P2) or rabbit (7074P2) IgG antibodies (Cell Signaling, Danvers, MA, USA) (Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat # 69295) at (1:1000), anti-Rab27B (E4V30, Cat # 17572), and α-Tubulin Mouse mAb (DM1A, Cat#3873) all purchased from Cell Signaling Technology. The anti-Rab27B (Cat # 13412-1-AP ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mouse anti-GAPDH (D4C6R; #97166), rabbit anti-FAS (#3189), and rabbit anti-XBP-1s (D2C1F, #12782S) were obtained from Cell Signaling (Danvers, MA). Mouse anti-calnexin (C8.B6 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation for 1 hour at room temperature in the dark in with secondary antibodies (Anti-rabbit: IgG DyLight 800, Anti-mouse: IgG DyLight 680 (Cell Signaling Technologies, USA)) ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed three times with TBST and incubated in secondary antibodies accordingly: anti-mouse HRP-linked IgG (Cell Signaling Technology, 7076S) or anti-rabbit HRP-linked IgG (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and intracellular location of 4H2 or IgG control was detected by immunostaining with an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Cell Signaling, Danvers, MA) as previously described (32) ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were washed in TBS containing 0.1 % Tween20 and labeled with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or anti-rabbit antibody (Cell Signaling Technology, Beverly, MA). Proteins were enhanced by chemiluminescence (Amersham ECL plus Western Blotting detection system ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were washed three times with PBST and incubated with either 1 μg/ml of rabbit monoclonal anti-mouse TREM2 (Cell Signaling Technology, 76765) for full length Trem2 ELISA (ELISA 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology, USA) secondary antibodies conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Genomics 2023Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: TP53 (DO-1, 1:500, Cell Signaling #18032, mouse), CDKN1A (F-5 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse-HRP, Cell Signaling #7076; anti-rabbit-HRP, Cell Signaling #7074) in blocking solution (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were treated with an HRP-linked secondary antibody (Anti-rabbit IgG cat. no. 7074S or Anti-mouse IgG cat. no. 7076S; Cell Signaling Technology, Inc) for 2 hours at room temperature ...