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Citations for Cell Signaling Technology :
2151 - 2200 of 10000+ citations for 7 Diethylamino 3 ethylamino 2 methylphenoxazin 5 ium tetrachlorozincate 2 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were dissolved with 3% BSA in tris buffered saline (TBST) and membranes were incubated overnight at 4°C with SQSTM1/p62 (Cell Signaling, cat# 88588, 1:1000). The primary LC3 A/B (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% milk in TBS/0.1% Tween-20 (TBST) and incubated with anti-phospho-eNOS (Thr495) and anti-eNOS (Suppl Table 3; 1:1,000, Cell Signaling cat. #9574 and 9572, respectively). Membranes were washed in TBST ...
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bioRxiv - Developmental Biology 2023Quote: We used an antibody for phospho histone 3 (pH3) that was previously reported(Shirae-Kurabayashi et al, 2006) (pH3-ser10-6g3-mouse-mAb, Cell Signaling; #9706; 1:500 diluted). The primary antibody was added together with anti-DIG antibody during FISH process ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Cancer Biology 2024Quote: Quantification of hepatocyte and tumor cell apoptosis was performed in liver slides by anti-cleaved caspase-3 immunostaining (Cell Signaling Technology #9661, diluted 1:400), detected with HRP-bound goat anti-rabbit secondary antibody (Invitrogen #31460 ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... YAP (Cell Signaling Technology, 14074S; 5 µl), Pan-TEAD (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL CDCP1 antibody (D1W9N, Cell Signaling) was added into 200 µL cell lysate precleared by 20 µL Protein A magnetic beads (EMD Millipore ...
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bioRxiv - Genetics 2022Quote: ... rabbit anti-MDA-5 (Cell Signaling, D74E4) and mouse anti-Actin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... or 5 μg Rabbit IgG (Cell Signaling) respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... Phospho-Smad1/5 (#9516S, Cell Signaling Technology), alpha-Tubulin (#2144S ...
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bioRxiv - Immunology 2023Quote: ... pSmad1/5/9 (Cell Signaling Technology, 13820S,), β-catenin (Cell signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... anti-phosphoSMAD1/5/9 (Cell signaling #13820), mouse anti-BMPR2 (BD Pharmingen ...
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bioRxiv - Microbiology 2024Quote: ... MDA-5 Rabbit mAb (Cell signaling, D74E4), Fibrillarin/U3 RNP Rabbit pAb (ABclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... docetaxel (5 nM; Cell Signaling Technology, #9886), or etoposide (25 μM ...
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bioRxiv - Systems Biology 2023Quote: ... 5 μM RPL30 Primer (Cell Signaling #7014) 2 μl or 100 μM target primers 0.4 μl and Taq DNA Polymerase 0.5 μl with the following PCR reaction program (Initial denaturation 95 °C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and 5% normal goat serum (Cell Signaling) and immunostained with specific primary antibodies or a cocktail of primary antibodies (Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal (4911, Lot 5, Cell Signaling); TAZ ...
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bioRxiv - Molecular Biology 2024Quote: ... MET pY1234/5 (3077) (Cell Signaling Technologies).
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bioRxiv - Cell Biology 2020Quote: ... Transferred blots were blocked for 1 hour in 5% w/v non-fat dry milk in 1X TBST (Cell Signaling Cat# 9997). Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858) ...
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bioRxiv - Cell Biology 2020Quote: For immunoblotting the following primary antibodies were used at a concentration of 1:1000 in 5 % milk-TBS-T: HA (Cell Signaling 3724S), HA (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...
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bioRxiv - Molecular Biology 2020Quote: ... After 3 washing steps (5 min each) with PBS-T membranes were incubated with horseradish-linked goat anti-rat (1:2000 dilution; 7077S; Cell Signaling Technology) or goat anti-rabbit (1:2000 dilution ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... except cells were cross-linked with formaldehyde (1%) for 5 min and cross-linked chromatin immunoprecipitated using an anti-HA rabbit monoclonal antibody (Cell Signaling Technology). Knock-in ESCs were first adapted to serum-free 2i/LIF condition as described before ChIP (15) ...
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bioRxiv - Cell Biology 2019Quote: ... Filters were incubated at 4 °C with primary antiserum diluted in 5% milk TBST as follows: FLAG antiserum (1:1000, Cell Signaling Technology), α-tubulin antiserum (1 ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim milk for 1 hr and incubated overnight with one or more primary antibodies: phospho-Histone H3 (Cell Signaling, 3377S), α-tubulin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in TBST plus 5% non-fat milk and then incubated with the following primary antibodies: phospho-S6 (S235/236; 1:2000, Cell Signaling, #4858), S6 (1:2000 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added overnight in 5% milk in TBS + 0.1% tween-20 (TBST): huntingtin (Clone D7F7, Cell Signaling 5656, 1:1,000), beta-actin (Clone 13E5 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000, Cell Signaling Technology, 67B9), rabbit anti- Pten (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were dissociated into single cells by TlypLE select and suspended in PBS containing the DNA dye DRAQ-7 (Cell Signaling). Using FACS Aria IIIu flowcytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 X 10^5 cells were incubated with EGFR (D1D4J) XP Rabbit antibody (PE Conjugate) (Cell Signaling Technology, 48685) for 1 hr at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... dissolved in in 1x TBS with 1% Tween-20 (TBS-T) for 60 min and separately incubated with primary antibodies: anti-HA (Cell Signaling Technology 2367S, 1:2000 dilution in 5% BSA/TBS-T) and anti-beta-tubulin (Invitrogen 32-2600 ...
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bioRxiv - Neuroscience 2020Quote: ... PFA- or NBF-fixed sections were incubated with the following primary antibodies: rabbit anti-cleaved caspase 3-D175 (Cell Signaling, #9661, 1:100, PFA-fixed tissue), Ctip2 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with TBS and 3% horse serum and 0.25% Triton X-100 before being incubated with Alexa Fluor© 488 Anti-Rabbit IgG (Cell Signaling, cat#4412S, diluted 1:250) for 2 hours at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 minutes in TBS-T and incubated overnight in primary antibodies against phosphorylated Akt T308 (Cell Signaling Technologies #2965; diluted 1:1000), phosphorylated Akt S473 (Cell Signaling Technologies #9271 ...
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bioRxiv - Developmental Biology 2023Quote: ... portions of the entire pancreas were sectioned at 5μm thickness and ≥3 non-consecutive sections of each portion were stained with an antibody against insulin (1:100, Cell Signaling Technologies: 4590, Danvers, MA, USA), which was subsequently detected by biotinylated goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2019Quote: ... Western blots for detecting MAP kinase phosphorylation were probed with primary antibodies against phospho-MAPK1/3 (Thr202/Tyr204) and total MAPK1/3 (Cell Signaling Technology, #4370 and #4696, respectively). MAPK1 is also known as ERK2 or p42MAPK ...
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bioRxiv - Cancer Biology 2021Quote: ... and cleaved caspase-3 (Cat. No. 9664, Cell Signaling, Danvers, MA). β-Actin (Cat ...
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bioRxiv - Developmental Biology 2020Quote: Primary antibodies for IHC include cleaved Caspase-3 (Cell Signaling #9661), phospho-Histone3 (S10 ...