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Citations for Cell Signaling Technology :
2101 - 2150 of 10000+ citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cdc42 (1:1000, Cell Signaling), β-Catenin (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Snail (1:1000; Cell Signaling). All phospho-specific antibodies were diluted in TBS-T with 1% milk and following antibodies were used ...
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bioRxiv - Molecular Biology 2024Quote: ... SBDS (1: 1000, Cell Signaling), VASP (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... ATF4 (1/50 Cell Signaling). Specificity was determined by substituting primary antibody with unrelated IgG (Santa Cruz) ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Biochemistry 2021Quote: Cleaved-caspase-3 immunostaining was performed on 2 µm thick formalin-fixed paraffin sections in a 1:200 dilution according to the manufacture’s protocol (Cell Signaling Technology, Danvers, MA, USA). Pictures were taken on a slide scanner SCN4000 in a 20× magnification.
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 2%BSA overnight and was probed with antibodies against goat anti-FLAG (1:1000, Cell Signaling Technologies, D6W5B, #14793), alpha Tubulin (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.8mg total protein for each sample was digested with trypsin and purified over C18 columns for enrichment with the Symmetric Di-Methyl Arginine Motif Antibody (Cell Signaling #13563). Enriched peptides were purified over C18 STAGE tips46 ...
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bioRxiv - Immunology 2024Quote: ... while the supernatants were carefully removed and used for the sequential di-methyl-Arginine peptide enrichment (di-me-R symmetric, cat.13563S Cell Signaling Technology). Peptides were eluted from the beads twice at room temperature with 50 µL of 0.15% TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were blocked with 3% BSA in PBS-T for 30 min and then incubated for 1 h with the following primary antibodies (diluted in 1% BSA in PBS-T): anti-RPA (Cell Signaling, #2208, 1:200), anti-Rad51 (Santa Cruz ...
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bioRxiv - Neuroscience 2019Quote: ... anti-phospho-CaMKII (T286, 1:1000), anti-phospho-S6K (T389, 1:500) and anti-S6K (1:500) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-CaMKII (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3127, 1:1000), anti-Jak1 (6G4, 3344, 1:1000), and anti-N-Cadherin (D4R1H, XP 13116, 1:1000) from Cell Signaling Technology (Danvers, MA); anti-phospho-Pak1-2-3 (pSer141 ...
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bioRxiv - Neuroscience 2020Quote: ... and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000, Cell Signaling Technology, Leiden, The Netherlands) or superoxide dismutase 2 (SOD2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies against phosphoylated-AKTT308 (p-AKTT308, 1:500 dilution, #2965), p-AKTS473 (1:500 dilution, #4060), and AKT (1:1000 dilution, #4961s) were from Cell Signaling Technology (Danvers, MA). All other chemicals were of the analytical grade and obtained from the local chemical suppliers ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:1000, #2947), phospho-SAPK/JNK (Thr183/Tyr185, 1:500, #9251), and phospho-CHK2 (Thr68, 1:500, #2661) from Cell Signaling Technology (Danvers, MA); β-actin (C4 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were prepared in blocking buffer (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; ASC, 1:500 dilution, Cell Signaling Technologies; P2RY12 ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies used were p-ser129 alpha-synuclein (FujiFilm cat# 015-25191, 1:1000) and HO-1 (Cell Signaling cat# 26416, 1:200). Sections were allowed to incubate overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... GAPDH (1:4000, #2118), Phospho-Akt (Ser473) (1:500, #9271) and Akt antibody (1:1000, #9272) were obtained from Cell Signaling Technology (Beverly, MA). Human VEGFR3/Flt-4 antibody (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT#: 14782S, EZH2 1:250-1:500 Cell Signaling Technologies CAT# ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Cell Biology 2020Quote: ... the pellet was washed with PBS (–) and incubated on ice for 5 min in 0.1 ml cell lysis buffer (Cell Signaling Technology, Inc.). In the other conditions ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was then washed with Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and blocked in 5% nonfat dry milk (Cell Signaling Technology, #9999) diluted with TBS-T for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in TBXT buffer containing 5% non-fat milk and horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Cell signaling tech.) and detected by using ECL Western Blotting Substrate (Pierce).
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bioRxiv - Molecular Biology 2022Quote: ... 5 minutes each at room temperature with agitation and then incubated with Anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling 7076S) in 2% milk powder in PBST and incubated at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... or 5 million cells for histone modifications was sheared with SFX250 Sonifier (Branson) and immunoprecipitated with antibodies targeting H3K27ac (#8173, Cell signaling Technology), H3K4me1 (#5326 ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were separated by electrophoresis on 10% or 12% SDS-polyacrylamide gels and transferred to PVDF membranes that were probed with anti-pSmad1/5/8 (Cell Signaling 9511S), anti-BMP4 (Santa Cruz sc12721) ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were diluted in either 5% goat serum/PBS-T or SignalStain Boost IHC Detection Reagent (8114S, Cell Signaling Technology (CST)) ...
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bioRxiv - Developmental Biology 2021Quote: ... Both Anti-ERVK-env mouse monoclonal (HERM-1811-5, Austral Biologicals, San Ramon, CA, USA) and anti-CDH1 rabbit monoclonal (Cell Signaling, 3195S) antibody were diluted 1:250 in PBS + 1% BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449}, H3K4me3 {Millipore 04-745} and H2AUb {Cell Signaling 8240S}) ...
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bioRxiv - Immunology 2020Quote: ... cells were cytospun 5’ at 500 rpm and senescence-associated beta-glalactosidase activity measured as recommended by the manufacturer (Cell Signaling Technology) on a Zeiss-Axio inverted phase-contrast microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... NCI-H522 and NCI-H1299 cells were treated with 5 μg/mL Poly I:C sodium salt (Cell Signaling Technologies Cat No. 61401) for 6 and 12 hours respectively.
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were then washed three times for 5 min with PB and mounted with a cover glass using ProLong (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... the fixed cells were blocked in PBS containing 5% bovine serum albumin (BSA) and then incubated with mouse anti-HA antibody (6E2, Cell signaling Technology) with 1:800 dilution overnight at 4°C ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocyte activation to a neurotoxic phenotype was induced in cells at 4-5 DIV by incubation with TNFα (25 ng/ml; Cell Signaling Technology), IL-1α (3 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Systems Biology 2024Quote: ... anti-MHC Class I (clone EMR8-5, cat#: 88274) and anti-PSMB9 (clone E7J1L, cat#: 87667) were purchased from Cell Signaling Technology. Puromycin (cat# ...