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Citations for Cell Signaling Technology :
2001 - 2050 of 2288 citations for Recombinant Mouse Clu His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Protein expression was measured by standard western blot procedures using anti-mouse primary antibodies against Arf6 (rabbit monoclonal; 1:1000; 19kDa; Cell Signaling), β-actin (mouse monoclonal ...
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bioRxiv - Physiology 2023Quote: ... Membranes were washed three times with TBS-T for 5 min and incubated with peroxidase-linked anti mouse or rabbit IgG at a 1:10,000 dilution in TBS-T (#7076/#7074S, Cell Signaling, Germany) for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were then washed three times with wash buffer and incubated 75 min at room temperature with a 1:10,000 dilution of goat anti-mouse HRP conjugated antibody (Cell Signaling Technology). After three washes with wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... Protein accumulation in the purified fractions was confirmed with western blot using anti-MBP tag (8G1) mouse monoclonal antibody (Cell Signaling) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology, 76765,) referred to as αTrem2-CT ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2,000 for IB) and mouse anti-Myc-tag (cat. #2276, RRID: AB_331783; 1:500 for IB) were purchased from Cell Signaling Technology. Rabbit anti-calnexin (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining employed anti-mouse IgG and anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technologies, #7076 and #7074, respectively) were used at 1:5000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: Mouse anti-MYC (2276), rabbit anti-HA (3724) and rabbit anti-FAM134B (83414S, for immunoprecipitation) were purchased from Cell Signaling Technology. Rabbit anti-FLAG (F3165) ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies were probed with 1:1000 Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, cat. No. 4408S), Alexa Fluor 594 conjugated anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies were probed with 1:1000 Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, cat. No. 4408S), Alexa Fluor 594 conjugated anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in HRP-linked anti-mouse or anti-rabbit IgG antibody for 1h at 24°C with gentle rocking (1:5,000 dilution, Cell Signaling Technology). Membranes were washed in TBST and protein bands were visualized using the Pierce ECL detection system (Cat #32106 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated with horse radish peroxidase-linked anti-mouse or anti-rabbit secondary immunoglobulin antibodies (1:10000; Cat# 7076S, Cat# 7074S Cell signaling) in PBST for 40 mins ...
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bioRxiv - Cell Biology 2023Quote: ... to the following proteins were used for the immunodetection of proteins using horseradish peroxidase-conjugated anti-mouse (Cytiva, NA931) and anti-rabbit (Cytiva, NA934) secondary antibodies (1:1,000): CHOP (Cell Signaling, 5554), Cleaved Caspase-3 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Catalog# 7076S) for ZIP4 or an HRP-conjugated anti-rabbit immunoglobulin-G for β-actin at 1:5000 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody was either goat anti-rabbit (7074) or horse anti-mouse (7076) IgG conjugated with horseradish peroxidase (Cell Signaling Technology). Staining was developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Biochemistry 2023Quote: ... Overexpression of Flag-NUDT15 and HA-NUDT18 were confirmed by analyzing cell lysates by SDS-PAGE followed by Western blotting using anti-FLAG-tag Rabbit antibody (#740001, Thermo Fischer Scientific) and anti-HA-tag (6E2) Mouse antibody (#2367, Cell Signaling) followed by anti-Rabbit HRP or anti-Mouse HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 5X with cold TTBS and incubated with anti-Rabbit or anti-mouse secondary antibodies conjugated to HRP (#7074 and #7076, Cell Signaling). After washing ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with a respective secondary HRP-conjugated antibody (anti-rabbit: Cell Signaling Technologies, #7074S, anti-mouse: Cell Signaling Technologies, #7076) for one hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and stained with appropriate antibodies: anti-NF-κB p65 and Alexa488-conjugated anti-mouse lamin A/C antibodies (both from Cell Signaling) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were anti-rabbit and anti-mouse IgG HRP-linked antibodies (1:5000, Cell Signaling, 7074 and 7076 respectively). Images were developed using the MINI-MED 90 X-Ray Film Processor (AFP Manufacturing Corp) ...
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bioRxiv - Biochemistry 2024Quote: ... which were detected with HRP-conjugated horse anti-mouse immunoglobulin-G antibody at 1:5000 dilution (Cell Signaling Technology, Cat# 7076) or goat anti-rabbit immunoglobulin-G antibody at 1:3000 dilution (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were washed for 15 minutes in TBST and incubated with horseradish peroxidase-conjugated anti-mouse IgG (Cell Signaling; cat#: 7076) or IgM (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk in TBST at room temperature for 60 min and probed with 1:1000 mouse-anti-E-cadherin antibody (#14472, Cell Signaling), 1:1000 rabbit-anti-Vimentin antibody (#5741 ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed with chilled FACS buffer (DPBS with 1% FBS and 2mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... in TBST and then probed with listed antibodies diluted in either 1% BSA (anti-phospho-specific antibodies; MPI) or milk and anti-rabbit-HRP or anti-mouse-HRP (Cell Signaling Technology) where appropriate.
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2019Quote: ... 11A5(Anderson et al., 2006) (mouse monoclonal 11A5 kindly provided by Imago Pharmaceuticals, 1:25,000) and D1R1R (rabbit mAb #20706S, Cell signaling. 1:1000), NF-L (mouse mAb #2835 ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed an additional three times for five minutes each before incubating in a 1:2000 dilution of anti-mouse horseradish peroxidase conjugated antibody (Cell Signaling Technologies) in TBST ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl permeabilization medium with 1:100 diluted Alexa Fluor 647-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology) and incubated for 30 min in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...