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Citations for Cell Signaling Technology :
2001 - 2050 of 9010 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG ...
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bioRxiv - Pathology 2022Quote: ... The blotted membranes were incubated with the following primary antibodies: rabbit monoclonal anti-phosphorylated Smad2 (Ser465/467) antibody (Cell Signaling Technology, #3108), rabbit monoclonal anti-Smad2 antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit monoclonal antibody (Cat. no. 4970) and anti-GAPDH (14C10) rabbit monoclonal antibody (Cat. no. 2118) were purchased from Cell Signaling Technology. Anti-β-tubulin rabbit polyclonal antibody (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... the primary antibody type (rabbit) was switched to mouse by using a conformation-specific mouse anti-rabbit antibody (Cell Signaling Technologies #3678) that only recognizes non-denatured antibodies to reduce the background from the heavy and light chains of the denatured IP antibody ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were then washed three times in TBST and incubated with horseradish peroxidase-conjugated anti-Rabbit IgG secondary antibody (All antibodies from Cell Signaling Technology) at 1:2000 dilution in TBST containing 5% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... or α-actinin1 with the corresponding primary antibody and an Alexa Fluor 488-coupled secondary antibody (Cell Signaling Technology #4408, 1/500) or an Alexa Fluor 546-coupled secondary antibody (Molecular Probes A11056 ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Equal protein loading was verified by stripping off the original antibodies and re-probing the membranes with a different primary antibody such as anti-GAPDH (Cell Signaling Technology). Pertussis toxin (PTX ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit anti-phospho-ERK (Thr202/Tyr204) monoclonal antibody (#4370) and rabbit anti-phospho-Akt (Ser473) monoclonal antibody (#4060) were purchased from Cell Signaling Technologies. Mouse anti-PtdIns(4)P monoclonal antibody (Z-P004 ...
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bioRxiv - Cell Biology 2021Quote: ... the antibody labeling steps were repeated on the same membrane for the rabbit anti-β-Actin antibody (Cell Signaling Technology Cat# 4967). The β-actin antibody was diluted 1:1000 in blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... blocked using 5% milk in TBST for 1 hour followed by primary antibody incubation at 4°C for overnight using the following antibodies at the appropriate dilutions– LC3 (1:1000, #2775, Cell Signaling Technologies), Atg5 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-phospho-Akt rabbit monoclonal antibody (Cat. No. 4060) and anti-phospho tyrosine rabbit monoclonal antibody (Cat. No. 8954) were purchased from Cell Signaling Tech. Anti-phosphotyrosine mouse monoclonal antibody (PY20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The CREB antibody 48H2 (Cat. No. 9197S) and phospho-CREB (Ser133) antibody 87G3 (Cat. No. 9198S) were purchased from Cell Signaling Technology, Inc.
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... were obtained at 30 h pt and incubated overnight with the anti-CD2v monoclonal antibody mix or anti-HA antibody (3724; Cell Signaling Technology). Pull down products were obtained by eluting in Laemmli buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were hybridized with the following primary antibodies as indicated: TYROBP (1:500, antibody provided in collaboration with Dr Richard W. Cho at Cell Signaling Technology); AT8 anti-pTAU pSer202/Thr205 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Following these acute treatments the cells were all then immunostained with an cell surface-EGFR antibody conjugated with FITC secondary antibody (Cell Signaling Technology) in Hibernate media (BrainBits ...
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bioRxiv - Physiology 2021Quote: ... A mouse primary anti-CaSR monoclonal antibody (1:2000, Gentex, Zeeland, MI) or a mouse primary anti-Myc (9B11) monoclonal antibody (1:1000, Cell Signaling Technology) was applied followed by a secondary horseradish peroxidase-coupled secondary antibody (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... and immunoblotted for ubiquitin as per standard protocols (anti-ubiquitin antibody P4G7, BioLegend #838701, 1:1000; anti-mouse IgG HRP-linked antibody, Cell Signaling Technology #7076 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody solution contained a 1/1000 dilution of a monoclonal rabbit anti-HA antibody (C29F4, Cell Signaling Technology, Danvers, USA) in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly-ADP-ribose polymerase (PARP) antibody and goat anti-rabbit IgG secondary antibody conjugated to horseradish peroxidase (HRP) were acquired from Cell Signaling Technology-Ozyme (Saint-Quentin-en-Yvelines ...
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bioRxiv - Immunology 2022Quote: ... The following horseradish peroxidase (HRP) conjugated secondary antibodies were used for this study: mouse anti-rabbit IgG (L27A9) monoclonal antibody (Cell Signaling #5127), goat anti-rabbit IgG polyclonal antibody (Cell Signaling #7074S) ...
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bioRxiv - Microbiology 2022Quote: ... The primary antibody was diluted to 0.1 mg/mL and anti-rabbit IgG HRP linked secondary antibody diluted 1:1000 (Cell Signaling Technology; 7074S).
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bioRxiv - Physiology 2023Quote: ... Bound primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse or -rabbit secondary antibodies (Cell Signaling Technology, Inc., Danvers, MA), and protein signals were visualized using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... or DIV12 neurons in the presence of recombinant protein (see recombinant protein below) or C1q-blocking antibody (Recombinant Anti-C1q antibody (ab182451)) / IgG (Normal Rabbit IgG,Cell Signaling Technology). For LPS induced activation of microglia ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Rabbit Secondary Antibody in 1:30000 dilution and Anti-Mouse Secondary Antibody (Cell Signaling Technology, 5151; Li-Cor, 926-68020, respectively) were used in 1:15000 dilution during Western Blotting experiments.
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Clones with successful deletions based on the PCR result were then analyzed using Western blots with antibodies against FOS or EGR1 and pERK antibody (Cell Signaling Technology) to identify clones that lack expression of the respective TFs and still induce MAPK signaling when the RAF1-CR3 kinase domain is activated with 4OHT ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained CD45 rabbit monoclonal antibody (D3F8Q, catalog #70257) and F4/80 XP rabbit monoclonal antibody (D2S9R, catalog #70076 from Cell Signaling Technology.
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution using the following concentrations: 1:1000 anti β-actin (13E5) rabbit primary antibody (#4970 from Cell Signaling), 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara) ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution (1:1000 anti β-actin (13E5) rabbit primary antibody #4970 from Cell Signaling) (1:1000 anti GAPDH ...
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bioRxiv - Genetics 2024Quote: ... anti-Cavin-1 antibody (#69036) and anti-MMP9 (D6O3H) XP Rabbit mAb antibody (#13667) were purchased from Cell Signaling Technology (Danvers, MA). pLenti-CMV-HA-HHIP lentiviral expression plasmid was generated by subcloning the appropriate human HHIP cDNA PCR fragment into pLenti-CMV Blast (659–1 ...
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bioRxiv - Systems Biology 2023Quote: ... Anti-caspase-8 antibody (4790, dilution 1:1000) and anti-cleaved caspase-8 antibody (8592, dilution 1:500) were purchased from Cell Signaling Technology. Anti-p-RIP3 antibody (ab222320 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Biochemistry 2022Quote: ... 30-50 μg of chromatin was used per sample and incubated with antibodies in a total volume of 600 μL overnight at 4° C with H3K27me3 antibody (Cell Signaling, 9733) or H4K20me0 antibody (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-phospho-mTOR (Ser 2448) rabbit antibody and anti-phospho-mTOR (Ser 2481) rabbit antibodies (Cell Signaling; Cat:2972, 2971, and 2974). TPC1 and TPC2 were detected using anti-TPCN1 and anti-TPCN2 rabbit antibodies (Alomone Labs ...
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bioRxiv - Developmental Biology 2023Quote: ... in PBS and incubated overnight at 4 °C with primary antibody or isotype control antibody diluted in PBS containing 10% donkey serum (Jackson) or goat serum (Cell Signaling Technology). Secondary antibodies (1:1000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked and probed with the indicated antibodies (Rb Antibody Sampler Kit #9969) and β-actin from Cell Signaling Technologies (Danvers, Massachusetts, USA).
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Genetics 2023Quote: ... The following antibodies and reagents were purchased and used in this research: Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) antibody from Cell Signaling Technology, Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Remove buffer and replace with 100μL DBE supplemented with primary antibody of interest (data presented here: rabbit anti-H3K27Me3 antibody (Cell Signaling, 1:100). Incubate cells plus antibody angled sideways on orbital shaker ...
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bioRxiv - Cancer Biology 2023Quote: ... which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S) ...
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bioRxiv - Immunology 2023Quote: ... The cells were then suspended in 50 µl antibody buffer pre-mixed with an anti-Flag antibody (1:50; Catalog:14793, Cell signaling Technology) or with anti IgG control (Catalog:2729S ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated with one of the following primary antibodies in antibody dilution buffer at 4°C overnight: anti-YAP1 (1:2,000 dilution; DBH1X; Cell Signaling, Danvers, MA), anti-CDX2 (1:100 dilution ...
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bioRxiv - Immunology 2023Quote: ... Rabbit monoclonal anti-FLAG (D6W5B) antibodies (Cat # 14793S) and Mouse monoclonal anti-MYC (9B11) antibodies (Cat# 2276S) were obtained from Cell Signaling Technology. Mouse polyclonal anti-V5 antibodies were purchased from MBL Life Science (Cat# PM003) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT# ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk and incubated with primary antibodies overnight and incubated with anti-rabbit secondary antibody from Cell Signaling Technologies for one hour after which blots were developed using Bio-Rad ChemiDoc™ Imaging Systems ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-linked goat anti-rabbit IgG (H+L) antibody (7074) and HRP-linked goat anti-mouse IgG (H+L) antibody (7076) were purchased from Cell Signaling Technology. Anti-mCherry antibody (SAB2702291) ...