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Citations for Cell Signaling Technology :
2001 - 2050 of 2711 citations for Mouse Neuropeptide FF NPFF CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk in TBST at room temperature for 60 min and probed with 1:1000 mouse-anti-E-cadherin antibody (#14472, Cell Signaling), 1:1000 rabbit-anti-Vimentin antibody (#5741 ...
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bioRxiv - Neuroscience 2024Quote: K-ε-GG peptide enrichment was performed using a K-ε-GG-specific antibody (PTM-Scan ubiquitin remnant motif K-ε-GG kit, Cell Signaling Technology, Kit#5562) chemically crosslinked to agarose beads as previously described (Udeshi et al. ...
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bioRxiv - Neuroscience 2024Quote: ... K-ε-GG peptide enrichment was performed using the K-ε-GG-specific antibody (PTM-Scan HS Ubiquitin/SUMO remnant motif K-ε-GG kit, Cell Signaling Technology, Kit#59322) covalently linked to magnetic beads ...
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bioRxiv - Cancer Biology 2020Quote: ... and diaminobenzidine substrate kit (#8059, Cell Signaling Technologies, Beverly, MA, USA) were used for immunohistochemistry ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-GAPDH (sampler kit from Cell signaling technology, US; 1:1000), horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (all purchased from Cell Signaling Technology) ...
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bioRxiv - Neuroscience 2019Quote: RapGAP activities were assessed using Active Rap1 detection kit (Cell Signaling) (82 ...
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bioRxiv - Cell Biology 2020Quote: BrdU Cell Proliferation Assay Kit (Cell Signaling Technology, Danvers, MA #6813) was performed as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... A DAB substrate kit (8059) was purchased from Cell Signaling Technology. Opti-MEM I reduced-serum medium (51985-034 ...
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bioRxiv - Developmental Biology 2021Quote: ... the SignalStain DAB substrate kit was used (Cell Signaling Cat# 8059) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... PTMScan Ubiquitin Remnant Motif Kit (Cell Signaling Technology, Cat. 5562, USA), C18 Sep-Pak SPE cartridge (Waters ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then developed with a DAB substrate kit (Cell Signaling, #8059).
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bioRxiv - Cell Biology 2023Quote: ... We utilized the SimpleChIP Enzymatic Chromatin IP kit from Cell Signaling following the manufactures protocol with a few exceptions ...
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bioRxiv - Molecular Biology 2023Quote: ... using the PTMScan Acetyl-Lysine Motif Kit (Cell Signaling Technology, 11663V1). The flow-through of the Acetyl-Lysine immunoprecipitation was kept for phosphorylated peptide enrichment ...
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was measured using XTT assay kit (Cell Signaling Technology) using a 96-well format.
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bioRxiv - Cancer Biology 2023Quote: ... PTMScan Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, 13416), GSH/GSSG detection assay (Beyotime ...
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bioRxiv - Molecular Biology 2023Quote: ... A senescence β-galactosidase staining kit (Cell Signaling Technology, Cat# 9860) was employed to score for senescence ...
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bioRxiv - Microbiology 2024Quote: ... Autophagy Antibody Sample Kit (4445T) was purchased from Cell Signaling Technology. Lyso-Tracker Red (C1046) ...
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bioRxiv - Cell Biology 2024Quote: ... the Bromodeoxyuridine (BrdU) Cell Proliferation Assay Kit (#6813, Cell Signaling Technology), resazurin reduction (alamarBlue™ Cell Viability Reagent ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed with chilled FACS buffer (DPBS with 1% FBS and 2mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... in TBST and then probed with listed antibodies diluted in either 1% BSA (anti-phospho-specific antibodies; MPI) or milk and anti-rabbit-HRP or anti-mouse-HRP (Cell Signaling Technology) where appropriate.
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2019Quote: ... 11A5(Anderson et al., 2006) (mouse monoclonal 11A5 kindly provided by Imago Pharmaceuticals, 1:25,000) and D1R1R (rabbit mAb #20706S, Cell signaling. 1:1000), NF-L (mouse mAb #2835 ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then washed an additional three times for five minutes each before incubating in a 1:2000 dilution of anti-mouse horseradish peroxidase conjugated antibody (Cell Signaling Technologies) in TBST ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl permeabilization medium with 1:100 diluted Alexa Fluor 647-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology) and incubated for 30 min in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... 5mg of lysate was incubated with 30ul of MyOne Streptavidin C1 Dynabeads or 5mg of Myc-Tag (9B11) Mouse mAb (Cell Signaling, #2276) antibody for 3 hours with subsequent 1-hour incubation with 30ul of Protein G Dynabeads for streptavidin pulldown and myc tag IP ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by a 1 h incubation in the HRP-labelled secondary antibodies (horse anti-mouse IgG (Cell Signaling Technology, Cat. No: 7076), and goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in TBXT buffer containing 5% non-fat milk and horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Cell signaling tech.) and detected by using ECL Western Blotting Substrate (Pierce).
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitated proteins were eluted from the beads using 150 µl of 1x Laemmli sample buffer for 5 min at +75□C and analysed by western blotting as described above except the HRP-conjugated mouse anti-rabbit native IgG secondary antibody (Cell Signaling, #5127) was used to prevent visualisation of heavy and light chains of co-eluted antibody.
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bioRxiv - Molecular Biology 2022Quote: ... 5 minutes each at room temperature with agitation and then incubated with Anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling 7076S) in 2% milk powder in PBST and incubated at room temperature for 2 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... The following secondary antibodies and their respective dilutions were used: Anti-mouse IgG(H+L) DyLight 800 conjugate (Cell Signaling Technologies, #5257), 1:20000 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed with tris-buffered saline with Tween 20 (TBST) three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and horseradish peroxidase-conjugated (HRP) anti-mouse and anti-rabbit secondary antibodies were all obtained from Cell Signaling Technologies (Beverly, MA, USA). Mini-protean precast Tris-Glycine Gels (TGX ...
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bioRxiv - Pathology 2021Quote: ... anti-Rnd3 (3664S), anti-Lamin B1 (12586S), anti-Myc-Tag (2276S), and mouse anti-rabbit IgG (Conformation Specific, 5127S) from Cell Signaling Technology, USA ...
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bioRxiv - Molecular Biology 2021Quote: ... Detection was performed with horseradish peroxidase-conjugated secondary antibodies: horse anti-mouse (1:5000; Cell Signaling, USA, Cat. No. Cat. No. 7076S), goat anti-rat (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were incubated with primary antibodies overnight at 40C and followed with the relevant HRP-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody cell signaling-7074 and Anti-mouse IgG, HRP-linked Antibody Cell signaling-7076) and visualized using Clarity Western ECL Substrate (BIORAD ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed and incubated in blocking buffer containing HRP-linked horse anti-mouse (1:5000; Cell Signaling Technology Cat#7076) for 45 minutes at room temperature ...