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Citations for Cell Signaling Technology :
2001 - 2050 of 2068 citations for 7 Diethylamino 3 2 thienyl coumarin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Primary antibodies to EGR1, EGR3, phospho-ERK1/2, GAPDH and actin, and an inhibitor of EphA2 (dasatinib, 100 nM) were from Cell Signaling Technologies. Secondary HRP-conjugated secondary antibodies used were goat anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was performed for 1h at room temperature in phospho-p44/42 MAPK (ERK1/2) (Thr202/Thr304) antibody (#4370, 1:200, Cell Signaling Technology) or human VE-Cadherin antibody (#AF938 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2023Quote: ... anti-PKM 1/2 (C103A3, #3190), anti-PKM2 (D78A4, #4053), and anti-pY849 PDGFRα/pY857PDGFRβ (C43E9, #3170S) antibodies were purchased from Cell Signaling Technology. Rabbit monoclonal anti-GLUT1 (ab115730) ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated rotating for 2 hr at 4°C with 1 uL anti-tri-methyl-histone H3 (Lys27) (C36B11, Cell Signaling Technology) or normal rabbit IgG (#2729 ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-phospho-p44/42 MAPK T202/Y204 (p-ERK 1/2, #9101, 1:1000), anti-PARP (#9532, 1:1000), and anti-LAMP1 (#9091, 1:400) from Cell Signaling Technologies and anti-Myc-Cy3 (Sigma) ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-phospho-SAPK/JNK antibody (#9251) and anti-phospho-p44/42 MAPK (Erk1/2) antibody (#9101) were obtained from Cell Signaling Technology (MA, USA). The PPARγ inhibitor ...
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bioRxiv - Biochemistry 2021Quote: Cleaved-caspase-3 immunostaining was performed on 2 µm thick formalin-fixed paraffin sections in a 1:200 dilution according to the manufacture’s protocol (Cell Signaling Technology, Danvers, MA, USA). Pictures were taken on a slide scanner SCN4000 in a 20× magnification.
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204, #4370; Cell Signaling Technology, Danvers, MA). Phosphorylation of Hog1 MAPK was detected using rabbit anti-Phospho-p38 MAPK antibody ((Thr180/Tyr182 ...
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bioRxiv - Cell Biology 2023Quote: ... hypoxia inducible factor 1 subunit alpha (HIF1A; 1:1000, no. #14179), Bax (1:1000, no. #2772), and Bcl-2 (1:1000, no. #4223) (Cell Signaling Technology, Inc., USA). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pEGFR (Y1068, #3777) pAKT (S 473, #9271 and p44/42 MAPK (ERK1/2) (T202/Y204, #4370) were purchased from Cell Signaling Technology (Danvers, MA), β- actin was used as a housekeeping proteinand the antibody was bought from EMD Millipore (#MAB1501 now part of Millipore Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) rabbit antibody (no. 3674, Cell Signaling Technology, 1:50 dilution); anti-E-cadherin rabbit antibody (no ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...