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Citations for Cell Signaling Technology :
2001 - 2050 of 2814 citations for 6 tert Butyl 4 chlorothieno 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... The membrane was washed 3 times in TBST and incubated for 1 hour at room temperature with HRP-conjugated anti-rabbit secondary antibody (Cell Signaling) diluted 1:1000 in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in TBST for primary or 3% BSA in TBST for secondary) were used for immunofluorescence: E-cadherin (#14472, monoclonal mouse, 1:100, Cell Signaling), mouse-IgG Alexa Fluor 594-conjugated secondary (#8890 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 × 10 min with TBST and incubated for 1 h with the appropriate secondary antibody (Cell Signaling Technologies) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were incubated with a polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at room temperature followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody with AEC+ as chromogen ...
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bioRxiv - Cell Biology 2021Quote: ... Protein accumulation rate upon proteasome inhibition was measured after 3-hour treatment with 10μM of MG-132 (Cell Signaling Technology #2194) in the RPMI medium.
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bioRxiv - Neuroscience 2020Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% normal serum: rabbit anti-cleaved caspase 3 (1/400, Cell Signaling, 9661), rabbit anti-doublecortin (1/2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse lung cryosections were stained overnight with anti-BrdU (1:3, BD Biosciences, or 1:1000 Cell Signaling Technology, Danvers, MA) and incubated with Alexa Fluo 488-conjugated secondary antibody (1:200 ...
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bioRxiv - Cell Biology 2021Quote: Human paraffin embedded pancreas biopsies were sectioned to 3 μm and immunostained with antibodies against MAFB (Cell Signaling Technologies, cat#41019), ARX (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: Human cancer tissue array staining was subjected to antigen retrieval at 98 °C for 20 min in EDTA buffer (pH 9.0) in a steamer and incubated with a rabbit anti-cleaved caspase 3 antibody (Cell Signaling, 9664L) 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012 ...
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bioRxiv - Microbiology 2020Quote: ... Lysed samples were centrifuged and incubated for 3 h with Pierce Protein A/G Magnetic beads (88802) which were pre-incubated over night with V5 antibody (Cell signaling E9H80 ...
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bioRxiv - Genomics 2019Quote: ... All wells were combined and filtered using a 30-μm CellTric (Sysmex) into a FACS tube and stained with 3 μM Draq7 (Cell Signaling). Using a SH800 (Sony) ...
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bioRxiv - Physiology 2020Quote: ... 1:100; rabbit anti-vinculin, 1:100, ab129002, Abcam, Cambridge, UK; rabbit anti-cleaved caspase-3, 1:400, 9446, Cell Signaling) were applied overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were incubated with the polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at RT followed by ImmPRESS Reagent Kit ...
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bioRxiv - Cancer Biology 2020Quote: Immunohistochemistry (IHC) was conducted on formalin fixed paraffin embedded tissues using cleaved caspase 3 antibody (1:300, Cell Signaling, Cat# 9661), EnVision+ Dual Link HRP secondary (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Neuroscience 2020Quote: ... and 8 μg of total protein subjected to immunoblotting against cleaved caspase-3 and −7 (1:200 dilution; RRID: AB_2341188 and RRID: AB_11178377; cat. no. 9661S and 8438S, Cell Signaling, Danvers, MA) as well as phosphorylated (1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3 times with TBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology #7074) for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... cleaved caspase 3 (9664, Cell Signalling, 0.5 μg ml-1) and cleaved-caspase 9 (9509, Cell Signaling, 1 μg ml-1). Secondary antibodies included Alexa Fluor 488 donkey anti-goat (A11055 ...
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... #9188),Ki-67 (1:1,000 dilution; Novocastra; NCL-KI-67-P) and Cleaved Caspase 3 (1:200 dilution; Cell Signaling, #9661). p-CREB 1:100 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for immunohistochemical studies included: anti-IGF-1R (#14534) and anti-cleaved-caspase 3 (#9661) from Cell Signaling Technology; and anti-CD99 (#ab-227738 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... The fixed and permeabilized cells were then labelled with antibodies against cleaved caspase 3 (Cell Signaling Technology; 9761; 1:500 dilution) and counterstained with DAPI ...
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bioRxiv - Genetics 2020Quote: ... and incubated overnight at 4°C in a 1:100 dilution of cleaved Dcp-1 antibody (Cell signaling #9578S) in PBSTx ...
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bioRxiv - Physiology 2019Quote: ... and equal amounts of protein were incubated overnight at 4 °C with rabbit acetyl-lysine antibody (AcK; Cell Signaling). Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology) ...
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bioRxiv - Immunology 2021Quote: ... Blots were incubated overnight at 4 °C with the corresponding primary antibody directed p-AKT (Cell Signaling Technology #9271), Akt (Cell Signaling Technology #9272) ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated at 4 °C with primary antibodies against rabbit phospho-ERK (1:200 D13.14.4E Cell Signaling®) and mouse NeuN (1:500 MAB377 MERCK® ...
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bioRxiv - Biochemistry 2020Quote: ... supernatant was mixing end over end for 1 hour at 4°C in mTOR IgG (2983, Cell Signaling Technology) or control IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4 °C overnight with the following antibodies: anti-PKR (1:1000; Cell Signaling cat. No. 3072), anti-p-PKR (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with the primary antibodies over night at 4°C: ATG5 (1:1000; Cell Signaling Technology, 2630s), ATG7 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated overnight at 4□°C with primary antibodies to galectin1 (1:100, Cell Signaling, #12936S) diluted in 5% NDS-1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... blots were probed with primary antibodies at 4°C overnight: rabbit anti-phospho-eIF2αS51 (Cell Signaling, #9721, 1:1000), rabbit anti-eIF2α(Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were incubated overnight at 4°C with anti-Myc-sepharose made with anti-Myc antibody (Cell signaling) and Pierce Crosslink IP Kit (Thermo/Pierce) ...
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bioRxiv - Pathology 2022Quote: ... Sections were incubated with rabbit monoclonal antibodies against olfactomedin-4 [D6Y5A] (Cell Signaling Technology Cat# 39141, RRID:AB_2650511, 1:400), Transferrin Receptor [EPR20584] (Abcam Cat# ab214039 ...
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bioRxiv - Cancer Biology 2022Quote: ... Then the cells were fixed in 4% paraformaldehyde and analyzed using an SA-β-Gal kit (Cell Signaling Technology).
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bioRxiv - Genomics 2022Quote: ... H3K27me3 immunoprecipitation was performed on 4 million cells with 10 µL of H3K27me3 antibody (Cell Signaling, C36B11 Lot #19). H3K9me3 immunoprecipitation was performed on 3 million cells with 8 µL of H3K9me3 antibody (Cell Signaling ...
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bioRxiv - Immunology 2019Quote: ... The following primary antibodies were used overnight at 4 °C: p-Syk (1:200, rabbit, Cell signaling technologies (CST), AB_2197222) ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently incubated with primary antibodies overnight at 4°C (anti-PKD1 (1:1000, Cell Signaling Technology #2052, RRID:AB_2172539), anti-pS916PKD (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were incubated overnight at 4 °C with rabbit acetyl-lysine (Ac-K, #9441) from Cell Signaling Technology. Protein densitometry was measured using Image J software (National Institutes of Health ...
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bioRxiv - Physiology 2019Quote: ... Samples were incubated overnight at 4°C with mouse and rabbit monoclonal antibodies against PCNA (Cell Signaling, 1:100) and Cleaved-CASP3 (Calbiochem ...
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bioRxiv - Developmental Biology 2019Quote: ... then incubated with appropriate primary antibodies overnight at 4°C: anti-cleaved NOTCH1 (Cell Signaling Technologies, 4147, 1:200), anti-NOTCH1 (Origene ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the HMLE cell line by chronic stimulation with 4 ng/ml TGFβ1 (Cell Signaling Technology) for 14 days9 ...
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bioRxiv - Pathology 2021Quote: ... Blots were incubated overnight at 4°C with mouse anti-FLAG tag mAB (Cell Signaling Technology, Danvers, MA, USA) at a final dilution of 1:1000 in blocking buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... was applied at 4°C overnight and secondary polyclonal goat anti-rabbit-HRP (Cell Signaling Technology #7074; 1:2,000) was applied for 1 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... lysates were ran out on 4-12% gradient gels and probed with anti-phospho(T389) p70S6K (Cell Signaling 9205) and anti-p70S6K (Cell Signaling 2708 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were applied for 24 h at 4°C: anti-LC3b (1:100; Cell Signaling Technology), anti-p62 (1:400 ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were incubated over night at 4 °C with primary antibodies against Phospho-SRC (Y416) (Cell Signaling Technology, 6943), SRC (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were incubated with the following primary antibodies overnight at 4 °C: SOX2 (1:1000, D9B8N, Cell Signaling), OCT4 (1:1000 ...