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Citations for Cell Signaling Technology :
1951 - 2000 of 3360 citations for Mouse Immunoglobulin G IgG CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Biochemistry 2020Quote: ... The following HRP-linked secondary antibodies were used by dilution in PBST: anti-rabbit IgG (Cell Signaling Technology, 7074S, 1:10,000), anti-mouse IgG (Zsbio ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed in TBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (1:2000; Cell Signaling Tech) diluted in blocking buffer for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The digested chromatin (500 μg) was subjected to immunoprecipitation with specific TCF7L2 antibody (Celling Signaling) or anti-Rabbit IgG (#2729 Cell signaling) as negative control overnight at 4° C with rotation and followed by ChIP-Grade protein G magnetic beads incubated for 2 hours at 4° C with rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then incubated for 30 min with a horseradish peroxidase-linked secondary antibody (anti-rabbit or antimouse IgG, Cell Signaling). The Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed three times with TBS containing 0.1% Tween 20 then incubated with anti-Rabbit IgG-HRP conjugated (Cell Signaling: 7074). Images were acquired by chemiluminescence using Clarity ECL (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed twice with PBST and incubated with HRP conjugated anti-rabbit IgG (1:500, catalog 7074S, Cell Signaling Technology) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... The secondary antibodies used for immunoblotting were anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) (Cell Signaling, 5151S) and anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed and then incubated with secondary antibody conjugated with IgG-HRP (cat#7074, Cell Signaling Technology, Boston, MA). Images were captured by ChemiDoc Touch Imaging System (Bio-rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Microbiology 2019Quote: ... and then incubated for 1 h with 1:1,000 dilution of secondary HRP-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology). Images of the immunoblots were developed with ECLTM Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... Thermo Fischer Scientific MA5-11690) overnight at 4°C and then incubated with goat anti-rabbit HRP linked IgG (1:10000, Cell signaling). Membranes were visualized using a ChemiDoc MP (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... All immunohistochemistry included an IgG isotype control and the primary antibodies we used were against phospho-Stat5 (Cell Signaling, Cat# 9314), β-casein (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... 2021) using 25μg of chromatin and 10ug of either anti-HIF1α ChIP antibody (Novus) or anti-rabbit IgG (Cell Signaling Technologies).Chromatin was immunoprecipitated overnight at 4C and HIF1α binding sites assayed via RT-qPCR using 2μl of either input ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... GAPDH-HRP (GTX627408-01, 1:120000) and P84 (GTX70220-01, 1:1000) The secondary antibodies were Anti-rabbit IgG (Cell Signaling, 7074 ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
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bioRxiv - Immunology 2023Quote: ... after which they were washed with PBS and incubated with an Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody (Cell Signaling) at room temperature for one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 60 µg of chromatin was incubated overnight on a rotating wheel at 4°C with the following antibodies: normal rabbit IgG (2729S, Cell Signaling), RNA polymerase II antibody (NBP2-32080 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed PBS tween before incubating with anti-rabbit IgG AlexaFluor® 647 conjugate (Cell Signaling Technology, #3452, RRID: AB_10695811) for 2 hours and washed again in PBS tween ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... 250 nM LEPTIN receptor antagonist Allo-aca (MCE #HY-P3212) or an equal volume of vehicle IgG (Cell Signaling Technology #2729). The formation of mineralized nodules was evaluated by alizarin red S staining ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:20000 (to detect GluN2C or GluN2A) or HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling, cat. #7074) at 1:40000 (to detect β-actin ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies against following proteins were used: MYPT1 (rabbit IgG mAb clone D6C1, #8574) from Cell Signaling Technology (Leiden, The Netherlands), MYPT1 (mouse IgG1 mAb clone C-6 ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Cancer Biology 2024Quote: ... The secondary anti-IgG rabbit antibody and the chemiluminescence system SignalFire™ ECL were from Cell Signaling Technology (Danvers, MA, USA). To normalize the total protein amount ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoprecipitation was performed with 2 µg rabbit polyclonal anti-KDM2B (gift from Dr. Robert Klose, Oxford University) and rabbit anti-RING1B antibody (1:100, Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Neuroscience 2022Quote: ... The remaining 90 μg of each sample was pooled for phospho-peptide enrichment with PTMScan Phospho-Enrichment IMAC FE-NTA Magnetic Beads (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and myc- and HA-tagged proteins were immunoprecipitated with Protein A/G beads (GeneBio, 22202B-1) coated with anti-myc antibody (Cell signaling, 2276S) at a dilution of 1:250 or anti-HA antibody (Cell signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2 x106/sample) were collected by centrifugation at 300 g for 4 min and resuspended in cell lysis buffer (Cell Signaling #9803) supplemented with protease inhibitors (Sigma #5056489001) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting mixtures of protein-DNA and antibody were further incubated with ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology). After sequential washing with low- and high-salt solutions ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Other antibodies include mouse anti-p53 Alexa fluor 647-conjugate (Cell Signaling), goat anti-GFP (Rockland) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1/10000) for cells, and Alix 3A9 (Mouse Monoclonal, #2171S (Cell Signaling), 1/1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse tissue was immunostained for GFP (Cell Signaling Technology 2956; 1:200), ChA (Abcam ab15160 ...
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bioRxiv - Cell Biology 2020Quote: ... or Alexa Fluor 647 (1:1,000, anti-mouse, Cell Signaling Technology, 4410). Mouse IgG (C2118 ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-mouse secondary antibody (1:3000, Cell Signaling Technology®, 7076S) for 2 hours at 25°C ...
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bioRxiv - Immunology 2021Quote: ... followed by anti-mouse-HRP (1:5,000, Cell Signaling, Cat. no: 7076S) secondary antibody.
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-S6 Ribosomal Protein (1:2000, Cell Signaling Technology, Cat# 2317), rabbit anti-Phospho-S6 Ribosomal Protein (Ser235/236 ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-S6 Ribosomal Protein (1:100, Cell Signaling Technology, Cat# 2317), rabbit anti-Phospho-S6 Ribosomal Protein (Ser235/236 ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used for western blotting were: anti-mouse (Cell Signaling, #7076), HRP-conjugated anti-rabbit (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... and mouse anti-phospho-Histone H3 (S10) (Cell Signaling; 9706S; 1:200) primary antibodies in PBS containing 0.3% triton X-100 and 1% BSA at 4 °C overnight ...
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bioRxiv - Systems Biology 2022Quote: ... mouse monoclonal anti-β-actin (8H10D10) (Cell Signaling Technology, cat. no. 3700S). After incubation ...