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Citations for Cell Signaling Technology :
1951 - 2000 of 2389 citations for Human Cytochrome P450 Family 4 Subfamily F Member 2 CYP4F2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice in 1 ml FACS buffer and resuspended in 50-100 μl of 1:50 diluted primary PE-conjugated antibodies against phosphorylated p38 or Erk1/2 (Cell Signaling Technology) and stained for 1 h at room temperature on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies to EGR1, EGR3, phospho-ERK1/2, GAPDH and actin, and an inhibitor of EphA2 (dasatinib, 100 nM) were from Cell Signaling Technologies. Secondary HRP-conjugated secondary antibodies used were goat anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was performed for 1h at room temperature in phospho-p44/42 MAPK (ERK1/2) (Thr202/Thr304) antibody (#4370, 1:200, Cell Signaling Technology) or human VE-Cadherin antibody (#AF938 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-phospho-p44/42 MAPK T202/Y204 (p-ERK 1/2, #9101, 1:1000), anti-PARP (#9532, 1:1000), and anti-LAMP1 (#9091, 1:400) from Cell Signaling Technologies and anti-Myc-Cy3 (Sigma) ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Genetics 2019Quote: ... the membranes were incubated for 12 hours or longer at 4°C with rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 1:1000 dilution) or mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked for 10 minutes in 10% BSA in TBS-T and then incubated overnight at 4°C with the indicated antibodies obtained from Cell Signaling Technology (phospho-PKC Substrate Motif (#6967), phospho-p42/44 MAPK (#4370) ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 independent controls and treated samples were probed with the following primary antibodies (in 5% milk, overnight at 4°C): YAP (1:500, Cell signaling Tec., Cat. No. 14074, RRID: AB_2650491), p YAP (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked in 5% evaporated milk in Tris-based saline with Tween 20 (0.05% TBST) for 1 hour and followed by incubating with primary antibodies overnight at 4 °C (Anti-HA: Cell Signaling Technology, 3724S; anti-GAPDH: Thermo, MA5-15738). After three rinses with TBST ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cancer Biology 2021Quote: ... using SimpleChIP Plus Enzymatic Chromatin Immunoprecipitation kit (agarose beads) (Cell Signaling Technology, 9004). The enriched DNA was quantified by qPCR analysis using the primers listed in Supplementary Table 3.
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... Primary antibodies used were: DNA damage antibody sampler kit (Cell Signaling Technology, #9947), PLK1 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2019Quote: ... the signals were detected with a chemiluminescence phototope-HRP kit (Cell Signaling, USA). As necessary ...
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bioRxiv - Molecular Biology 2019Quote: Senescence assays were performed using the Senescence b-Galactosidase Staining Kit (Cell Signaling) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ChIP assay was conducted by using the ChIP assay kit (Cell Signaling Technology) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... ChIP was performed with Simple ChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP was performed with the SimpleChip Plus Kit with magnetic beads (Cell Signaling) per manufacturer’s protocol with the following exceptions ...
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bioRxiv - Physiology 2020Quote: ... using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads; Cell Signaling). Chromatin lysates were prepared ...
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bioRxiv - Cancer Biology 2021Quote: ChIP assay was conducted by using the ChIP assay kit (Cell Signaling Technology) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... SignalStain® Boost IHC Detection Reagent and DAB substrate kit (Cell Signaling Technology) were used and then the sections were counterstained with hematoxylin ...
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bioRxiv - Physiology 2020Quote: ... using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads; Cell Signaling). Liver from pups and adult control and Alb-D2KO mice were obtained as described (22) ...
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bioRxiv - Cell Biology 2021Quote: DRIP was performed using the SimpleCHIP enzymatic chromatin IP kit (Cell Signaling Technologies) in accordance with manufacturer’s instructions with some modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Cell Fractionation Kit (9038) was purchased from Cell Signaling Technology (Danvers, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Detection was performed using DAB substrate kit (8059S, Cell Signaling Technology, Danvers, MA). Samples were counterstained using Mayer’s hematoxylin (MHS32 ...
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bioRxiv - Bioengineering 2022Quote: CUT&RUN was performed using the CUT&RUN Assay Kit (Cell Signaling #86652). Briefly ...
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bioRxiv - Physiology 2022Quote: ... Proliferation was measured using the BrdU Cell Proliferation Assay Kit (Cell Signaling Technology). Migration was measured using the ORIS Cell Migration Assay Kit (Platypus Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... ChIP assay was performed using the ChIP Assay Kit (Cell Signaling Technology, 9003) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... all antibodies were from the NF-kB Pathway Sampler kit (Cell Signaling, 9936S) and β-actin was from Abgent (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies were from NF-kappa B Pathway Sampler kit (Cell Signaling, 9936S). Immunodetection was performed with Chemiluminescent HRP Antibody Detection Reagent (Denville Scientific INC ...
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bioRxiv - Cell Biology 2022Quote: ChIP was performed using the SimpleCHIP enzymatic chromatin IP kit (Cell Signaling Technologies) in accordance with manufacturer’s instructions with some modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection was performed using DAB substrate kit (8059S, Cell Signaling Technology, Danvers, MA). Samples were counterstained using Mayer’s hematoxylin (MHS32 ...