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Citations for Cell Signaling Technology :
151 - 200 of 1350 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Neuroscience 2020Quote: ... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... with added protease and phosphatase inhibitors at a 1:100 concentration (Cell Signaling Technology). Protein concentration was determined using a commercial colourimetric assay (Bio-Rad Protein Assay kit II ...
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bioRxiv - Cell Biology 2022Quote: ... and calyculin A serine/threonine phosphatase inhibitor (10µL per mL of volume; Cell Signaling). Ovaries were homogenized on ice by grinding with an automated tissue grinder (KONTES pellet pestle ...
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bioRxiv - Immunology 2023Quote: ... 1 mM CaCl2 in H2O) supplemented with 1:100 protease/phosphatase inhibitor (Cell Signaling). The protein content was measured using a Pierce BCA assay kit (Thermo Scientific) ...
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bioRxiv - Immunology 2019Quote: ... 1 mM EDTA and 1x Protease/Phophatase inhibitor cocktail (Cell Signaling Technology). Cell lysates were incubated with V5 conjugated with protein G dynabeads for 2 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% Triton X-100) with 1x protease and phosphatase inhibitor (Cell Signaling Technology, 5872S) and protein concentration determined by Pierce BCA Protein Assay Kit (23225) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM of the prolyl hydroxylase inhibitor DMOG and 10 µM of the protease/phosphatase inhibitor MG-132 (Cell signaling; product 5872S) were used to prevent degradation of HIF ...
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bioRxiv - Cell Biology 2022Quote: Reagents used are as follows: protease inhibitors cocktail (Cell Signaling Technology, MA, USA), Penicillin-Streptomycin (Cat.# P4333 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM PMSF) supplemented with protease inhibitor cocktail (Cell Signaling Technology, Massachusetts, USA). The protein concentration was measured using Bradford protein assay (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in RIPA buffer with protease inhibitor cocktail (Cell Signaling Technology), and protein concentrations were determined by BCA assay (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with a protease inhibitor cocktail (Cat. No. 5871S, Cell Signaling, Danvers, MA), at 40C on a rocking platform ...
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bioRxiv - Cell Biology 2023Quote: ... 1× Protease inhibitor cocktail and incubated with H3K27me3 antibody (Cell Signaling Technology #9733), H3K27ac antibody (Active Motif #39134) ...
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bioRxiv - Neuroscience 2020Quote: ... 50 mM NaCl (pH 8) with a protease inhibitor (phenylmethylsulfonylfluoride, 0.5 mM final concentration and protease inhibitor cocktail from Cell Signaling Technology). Cells were lysed by sonication on ice for 10 min (20 s on ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in RIPA buffer supplemented with protease inhibitor cocktail (Cell Signaling Technology). Protein lysates were subjected to SDS/PAGE and immunoblotted with antibodies against AR (441 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 50 mM NaCl) containing protease inhibitor cocktail (cat# 5871, Cell Signaling Technology, Danvers, MA). To inhibit residual histone deacetylase (HDAC ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples were collected and lysed using RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC-1 cells were lysed in buffer containing protease and phosphatase inhibitors (Cell Signaling, catalog number: 9803S). Total protein content was quantified by Bradford assay and equal amounts of proteins were analyzed in duplicate with arrays containing 4 to 6 spots for each of 60 probes (ACC058 ...
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bioRxiv - Pathology 2021Quote: ... Tendons were homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease/phosphatase inhibitors (Cell Signaling Technology [CST] #58725) using 0.5-mm zirconium oxide beads and a Bullet Blender Gold Cell Disrupter (Next Advance Inc.) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S ...
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bioRxiv - Genomics 2019Quote: Protein lysates were obtained using ice-cold 1X RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling 9806), and freshly supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with Protease and Phosphatase Inhibitor for assessment of total Stat 1(Cell Signaling Technology, Danvers, Massachusetts, USA) as well as phosphorylated-Stat 1 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: The EV pellet was resuspended in RIPA buffer with protease/phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA). Protein (20 µg ...
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bioRxiv - Cancer Biology 2022Quote: ... The MEK1/2 inhibitor (U0126) was purchased from Cell Signaling and used at a 5 μM final concentration ...
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bioRxiv - Genomics 2020Quote: ... with each buffer supplemented with 1mM PMSF and 1x protease inhibitor cocktail (Cell Signaling Technologies, #5871).
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed with RIPA lysis buffer (Pierce) containing a cocktail of protease inhibitors (Cell Signaling). Equivalent amounts of proteins determined by the Bradford protein assay (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared using RIPA buffer containing cOmplete ULTRA protease and phosphatase inhibitor (Cell Signaling Technology (CST), Danvers ...
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bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue (n=3 tendon per sample) was homogenized in RIPA buffer (including 1X protease/phosphatase inhibitor, Cell Signaling #58725) to extract the total protein with the Biomasher II system and Powermasher II electric stirrer (Nippi Inc ...
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bioRxiv - Genetics 2019Quote: ... and resuspended in a lysis buffer with the addition of complete protease inhibitor cocktail (Cell Signaling Technology). Chromatin was sheared by sonication 3×5 cycles of 30 sec ON/30 sec OFF with high intensity (Bioruptor ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mM EDTA and 1 % Triton X-100) supplemented with protease inhibitor cocktail (Cell Signaling, Danvers, MA). After 10 min of incubation ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with protease inhibitor cocktail (PIC) at 1× (catalog number 5871; Cell Signaling Technologies, Inc., Danvers, MA) and stored at - 80°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed once with 0.5% BSA/PBS and once with PBS prior to be resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0, and 1% SDS) supplemented with protease/phosphatase inhibitor (Cell Signaling), and chromatin was solubilized by sonication in a Bioruptor (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: ... the inhibitor of MEK1/2/ERK1/2 kinases UO126 (used at 10 μM; 9903, Cell Signaling Technology), Smad3 inhibitor SIS3 (used at 6 μM ...
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bioRxiv - Immunology 2020Quote: ... 10 μM of p38 inhibitor (SB203580) or MEK1/2 inhibitor (U0126) was used (Cell Signaling Technology, Danvers, MA, USA). Cells were monitored by live cell imaging using the BD Pathway 855 Bioimaging System (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: The following small molecule inhibitors were used in this study: MEK inhibitors: 2 μM PD 184352 (Cell Signaling 12147) and 10 μM Selumetinib (Selleckchem S1008) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1× Protease inhibitor cocktail and incubated with indicated antibodies (Supplementary Table 3) or normal rabbit IgG (Cell Signaling) at 4 degrees overnight on nutator ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed by ice-cold RIPA buffer (150mM NaCl, 1% NP40, 0.1% SDS and 50mM Tris, PH7.4) containing protease and phosphatase inhibitor (Cell Signaling Technologies; 5872) for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with ice-cold PBS and lysed in 100 µL of phosphatase and protease inhibitor containing RIPA buffer (Cell Signaling Technology). Cells were incubated on ice for 30 min and then harvested by scraping followed by centrifugation at 14,000 rpm for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The MEK1/2 inhibitor U0126 was from Cell Signaling Technology (Danvers, MA, USA) (Cat# 9903S ...
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bioRxiv - Cancer Biology 2019Quote: Western blotting was performed using a routine protocol after cell lysis in RIPA buffer including protease and phosphatase inhibitors (#9806 and #5872 Cell signaling, Frankfurt, Germany). Grayscale intensity values were normalized to internal positive controls and measured using ImageJ software (NIH ...
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bioRxiv - Neuroscience 2023Quote: ... cultured primary pericytes were lysed with cold lysis buffer of radioimmunoprecipitation assay (RIPA) lysing buffer supplemented with 1x protease/phosphatase inhibitor (Cell Signaling Technology; U.S.A). Cells were mechanically agitated for 5 minutes and incubated on ice for a further 30 minutes with occasional agitation ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested post-treatment and re-suspended in lysis buffer (50⍰mM Tris-HCl pH 7.4, 150⍰mM NaCl, 1% NP-40, 1% protease inhibitor cocktail (Cell Signaling), sonicated ...
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bioRxiv - Molecular Biology 2021Quote: Tissue was collected and incubated on ice for 60 minutes using RIPA buffer containing 1X RIPA including a phosphatase inhibitor Na3VO4 (Cell Signaling, Leiden, the Netherlands), 1 mM phenylmethylsulfonyl fluoride (PMFS ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosphatase (Cell Signaling Technologies #5870S) inhibitors and incubated for 5-10 minutes on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The cultures were thawed on ice water and 1 mM phenylmethylsulfonyl fluoride [PMSF] with 0.1x protease inhibitor cocktail (Cell Signaling Technology) were added ...
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bioRxiv - Neuroscience 2022Quote: ... or (2) with a cocktail of rabbit anti c-Fos (1:500, Cell Signaling Technology, Cat. No. 2250) and mouse anti PV (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Biochemistry 2022Quote: ... Then the cells were washed with cold phosphate-buffered saline (PBS) and lysed using RIPA buffer with protease inhibitor cocktail (#5871S, Cell Signaling, Danvers, MA) and phosphatase inhibitor cocktail (#78420 ...