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Citations for Cell Signaling Technology :
151 - 200 of 206 citations for Osteocalcin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000, Cell Signaling; GCN5L1 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... blots were incubated for two hours in 5% milk in 1X TBST for blocking followed by an overnight incubation with rabbit anti-c-Fos antibody (1:1000, Cat. No. 2250, Cell Signaling Technology, USA) at 4°C ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The processed tissue was rinsed 3 times with PBS (20min each) to wash off the Dent’s Fix and then incubated with primary antibody in blocking buffer (pS6 Cell Signaling 2215, 1:1000) at 4°C with gentle rotation for 5 days ...
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bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: K-ε-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-Є-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described [62] ...
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bioRxiv - Molecular Biology 2021Quote: ... quantified with Quantitative Colorimetric Peptide Assay (Thermo Fischer Scientific) and dried on Speed-Vacuum before enrichment with Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling). After Sep Pak desalting ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for acetylation with anti-acetyl antibody conjugated to agarose beads (Acetyl-Lysine Motif Kit; Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: Concentrated stock of isotopically labelled internal standard (heavy) peptides (M1, M1ox, K6, K6ox, K11, K27, K29, K33, K48, K63) were purchased from Cell Signaling Technologies and Cambridge Research Biochemicals (Cleveland ...
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bioRxiv - Molecular Biology 2020Quote: ... the rabbit monoclonal anti-Actin antibody (12748S, rabbit monoclonal antibody to a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein, Cell Signaling) and the rabbit monoclonal anti-acetyl-histone H3 (Lys9 ...
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bioRxiv - Cell Biology 2021Quote: Acylated (either acetylated or malonylated) peptides were enriched by immunoaffinity purification using the PTMScan Acetyl-Lysine or PTMScan Malonyl-Lysine Kits (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining 90 μg of each sample was pooled for phospho-peptide enrichment with PTMScan Phospho-Enrichment IMAC FE-NTA Magnetic Beads (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Dry peptides were then resuspended in an IAP buffer (Cell Signalling #59322) and incubated with anti-diGly antibody-conjugated slurry (Cell Signaling #59322) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Enrichment for ubiquitinated peptides was conducted using a PTM Scan® Ubiquitin Remnant Motif [K-ε-GG] kit (Cell Signaling, Danvers, MA) following the vendor’s recommended procedures ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked in 4% FBS in 1x PBS + 0.1% Triton X-100 prior to incubation with primary antibodies diluted in blocking buffer: rabbit anti-γ-H2AX (Cell Signaling, 2577, 1:400); mouse anti-p53 (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were saturated with the blocking solution followed by a 1hr at 37°C incubation with the primary antibodies: Bcl-xL (Cell Signaling #2764, 1/1000) and IP3R C-terminal (abcam #ab190239 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with blocking solution for 90min then with a combination of two primary antibodies: rabbit anti-Fos (1:2000; Cell Signaling Technology, RRID #2250) and chicken anti-mCherry (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and incubation with the primary antibodies in the blocking buffer for overnight at 4°C was followed by a two-hour incubation with secondary antibodies (Cell Signaling, Danvers, MA, USA) in the blocking buffer at room temperature ...
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bioRxiv - Physiology 2020Quote: ... The blots were washed and immunoreactive bands were detected by incubation with anti-rabbit HRP conjugated antibody (dilution 1:2000 in blocking solution; Cell signaling, Cat no. 7074S) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were prepared in blocking buffer (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; ASC, 1:500 dilution, Cell Signaling Technologies; P2RY12 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilized with 0.5% Triton X-100 in blocking buffer for 15 min and incubated with primary antibodies against internal epitopes (DARPP32 (1:500, Cell Signaling Technology 2306, RRID:AB_823479) or PSD95 (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated in the following primary antibodies diluted in blocking solution: anti-E-cadherin (24E10, CST 3195, Cell Signaling, dilution 1:200), anti-ezrin (E1281 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Microbiology 2021Quote: ... and sera from individual animals were tested by ELISA with KLH-conjugated peptide immobilized on plastic surface and anti-mouse or anti-rabbit horse radish peroxidase (HRP)-conjugated detector Ab (Cell Signaling Technology, USA). The animals with the highest Ab titer were selected for booster immunization ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Western blots were then blocked with LI-COR blocking buffer and incubated overnight with primary antibodies anti-pCREB (Ser133)(Cell Signaling 87G3, #9198, 1:1000) and anti-GAPDH (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (LICOR, Omaha, NE, USA) and incubated overnight with an antibody against hypoxia inducible factor-1α (HIF-1α, Cell Signaling Technology #36169; 1:500). Proteins were visualized using IRDye 800CW secondary antibodies (LICOR ...
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bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole protein lysates were analyzed for total protein expression and lysine-methylated peptides were enriched using reagents from Cell Signaling Technology (catalogue no. 14809). In short ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for malonylation with anti-malonyl antibody conjugated to agarose beads from the Malonyl-Lysine Motif Kit (Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were then incubated at 4°C overnight in Odyssey Blocking buffer containing 0.1% Tween 20 and the following antibodies: ERK 1/2 (mouse monoclonal, Cell Signaling Technology no. 4696; 1:1000 dilution), pERK 1/2 (rabbit monoclonal ...
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bioRxiv - Microbiology 2023Quote: ... Wells were washed three times and incubated with HRP-labeled anti-mouse IgG H+L (1:2500 dilution in blocking buffer) (Cell Signaling Technology, Danvers, MA, USA, #7076P2). Plates were developed with 1-Step ultra TMB-ELISA substrate solution and incubated at room temperature for 15-30 min ...