Labshake search
Citations for Cell Signaling Technology :
151 - 185 of 185 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were blocked with 3% BSA in PBS-T for 30 min and then incubated for 1 h with the following primary antibodies (diluted in 1% BSA in PBS-T): anti-RPA (Cell Signaling, #2208, 1:200), anti-Rad51 (Santa Cruz ...
-
bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
-
bioRxiv - Cell Biology 2021Quote: ... then incubated for 1.5 h at RT with either CD36 (Novus NB400-144, 1:500 dilution) or PPARγ (Cell Signaling 2430S, 1:250 dilution) and GAPDH (Abcam ab181602 ...
-
bioRxiv - Cell Biology 2020Quote: SMC were seeded at a density of 75000/well in 6 well plates and cultured in FGM for 48 h prior to fixation and staining using a commercial β-galactosidase assay (Cell Signaling Technologies #9860) as described previously (41) ...
-
bioRxiv - Cell Biology 2020Quote: ... was performed for 24 h and cells were subject to immunofluorescence staining with an anti-BrdU antibody (1:400 dilution, Cell Signaling, cat no. 5292) before counterstained with DAPI and assessed by fluorescence microscopy.
-
bioRxiv - Cell Biology 2021Quote: ... was performed for 16-18 h and cells were subject to immunofluorescence staining with an anti-BrdU antibody (1:400 dilution, Cell Signaling, cat no. 5292) before counterstained with DAPI and assessed by immunofluorescence and high content analysis microscopy ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The membrane was washed four times in 0.1% TBST buffer and incubated for 1 h at room temperature with anti-rabbit IgG conjugated to HRP-linked antibody #7074 (Cell Signaling Technology, Danvers, MA) diluted 1:5,000 with 0.05% TBST containing 5% skimmed milk ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: 5 μM thick paraffin-embedded liver sections were used for hematoxylin and eosin (H&E) staining and Ki67 (Cell signaling technology 12202S, 1:400) immunohistochemical staining ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS-T and they were incubated with goat anti-rabbit IgG H&L Alexa Fluor 488 (Cell Signaling Technology, MA, USA) for 90 minutes at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... The secondary antibodies used were Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5151 and Anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5257 (Cell Signaling Technology) respectively ...
-
bioRxiv - Systems Biology 2024Quote: ... Membranes were blocked in 5% milk or bovine serum albumin (BSA) for 1 h at room temperature and incubated with primary antibodies against: PI3 Kinase Class II α (Cell Signaling, 1:2000), Phospho-AKT(Ser473 ...
-
bioRxiv - Physiology 2024Quote: ... membranes were washed 3x5 min in TBST and incubated for 1 h with HRP-conjugated anti-rabbit IgG (Cell Signaling Technology, Cat No. 7074), HRP-conjugated anti-mouse IgG (Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2024Quote: ... blocked with 5% BSA for 1 h at room temperature and incubated overnight at 4ºC with primary antibodies targeting phopsho-TBK1 (Cell Signaling, 5483, 1:1,000 dilution), TBK1 (Cell Signaling ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... or 1 h incubation with m-IgG Fc BP-HRP (sc-529765) followed by SignalStain DAB Chromogen detection (Cat. # 8059, Cell Signaling Technology, Danvers, MA) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: Cells were transfected with indicated plasmids for 24-48 h and harvested with Cell Lysis Buffer (cat: #9803; Cell Signaling Technology, Inc. Danver, MA) or RIPA Buffer (cat ...
-
bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated in secondary antibody (HRP conjugated anti-rabbit or anti-mouse IgG(H+L) (1:10,000 dilution, Cell Signaling, cat # 7074P2 or 32230, respectively). Subsequently the signal was developed using Super Signal West Femto substrate (ThermoFisher Scientific ...
-
bioRxiv - Physiology 2020Quote: ... China) at room temperature for 1 h before incubation with a mouse anti-human CD44 monoclonal antibody (Cat. #3570; Cell Signaling Technology, Danvers, MA, USA) and a rabbit anti-human CD24 antibody (Cat ...
-
bioRxiv - Cancer Biology 2024Quote: ... at room temperature for 1 h before application of primary antibody incubation at 1:500 dilution (Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (Cell Signaling Technology, Cat# 11818, RRID:AB_2687505)) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: Anti-Mouse IgG (H+L) Fab2 Alexa Fluor 488 Conjugate (Cell Signaling Technologies, Danvers MA, 4408S, dilution 1:50), Anti-Rabbit IgG (H+L ...
-
bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... at room temperature for 1 h and then incubated for 16 h with primary antibodies against the ER tracker protein disulfide isomerase (PDI; rabbit mAb #3501, Cell Signaling Technologies, Danvers, MA, USA) and a loading control GAPDH (rabbit mAb #2118 ...
-
bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
-
bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1% BSA for 1 h at 37°C and incubated with primary antibodies: anti-Flag (Cell Signaling, D6W5B, 1:1000 dilution), anti-GFP (Abcam ...
-
bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5% BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4-times for 10 min in 1 x PBS-T before 1 h incubation at room temperature with secondary anti-rabbit IgG coupled to horseradish peroxidase (Cell Signaling Technology, Danvers,MA, USA) diluted 1:1,000 in 1 x PBS-T ...
-
bioRxiv - Synthetic Biology 2021Quote: ... for 1 h at room temperature prior to incubation in primary antibody (Novus Biologicals, NBP2-59627 [1:1000 dilution]; Cell Signaling Technology, 2148 [1:1000 dilution]) solubilized in a 1:1 mixture of Odyssey blocking buffer and TBS-T buffer (50 mM Tris Base ...
-
bioRxiv - Microbiology 2023Quote: ... Wells were washed three times and incubated with HRP-labeled anti-mouse IgG H+L (1:2500 dilution in blocking buffer) (Cell Signaling Technology, Danvers, MA, USA, #7076P2). Plates were developed with 1-Step ultra TMB-ELISA substrate solution and incubated at room temperature for 15-30 min ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following primary antibodies were diluted 1:1000 in 3% BSA/T-TBS and incubated for about 16 h: anti LC3-I/II (cat. # 12741S) from Cell Signaling Technologies (distributed by Euro-Clone); anti-α-Tubulin (cat ...
-
bioRxiv - Cell Biology 2024Quote: ... The immunoblots were conducted using Polyvinylidene Fluoride (PVDF) membranes and were incubated for around 16 h with primary antibodies such as anti-p27KIP1 (Cell Signaling; distributed by EuroClone; cod. #3698), anti cdc2 (Santa Cruz Biotechnologies ...
-
bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% skim milk in TBST for 1 h at room temperature and incubated with anti-human p53 antibody (Cell Signaling Technologies, #9282, rabbit polyclonal, 1:1000) in TBST containing 5% skim milk (4°C ...
-
Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Then membranes were incubated for 1 h at RT with secondary anti-mouse or anti-rabbit HRP-linked antibodies (Cell Signaling Technology #7076 or # 7074; 1:5000) diluted in Bullet Blocking One and were washed three times for 15 min in 1×TBST buffer ...
-
bioRxiv - Bioengineering 2023Quote: ... washed three times for 10 min in PBS and incubated for 1 h in blocking solution (5% normal goat serum, Cell Signaling Technology #5425, 0,3% Triton X-100 in PBS) at room temperature ...