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Citations for Cell Signaling Technology :
151 - 200 of 233 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1 h incubation with the HRP-conjugated secondary antibody (Cell Signaling 7076S, 1:10,000 in 2.5% milk in TBS-T). After three washes in TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Genomics 2024Quote: The CD34+ cells were treated with TAK-243 for 48 h and the cells were collected and immobilized on magnetic beads conjugated with a specific antibody against ATF4 (Cell signaling; (D4B8) Rabbit mAb #11815 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed with TBS-T before staining with secondary anti-rabbit IgG (H+L) DyLight 800 4X PEG Conjugate (Cell Signaling #5151) at 1:20,000 at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Samples were blocked in 1 % BSA in PBS for 3 h in a hydration chamber followed by primary antibody staining with pStat1 (Tyr701, Cell Signaling #9167) at 1:200 dilution overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 16 h with primary antibody (PPARα, Santa Cruz Biotechnology cat# sc-398394; histone H3, Cell Signaling Technologies cat# 14269) diluted 1:1000 in tris-buffered saline (0.5% Tween ...
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bioRxiv - Cancer Biology 2024Quote: ... Fish with tumors were euthanized after anesthesia and characterized by histopathological hematoxylin and eosin (H&E) staining and immunohistological analysis or immunofluoresence using antibodies against PCNA (Cell Signaling, D3H8P), tyrosine hydroxylase (Pel-Freez Biologicals ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated for 1h in 100 μL PBST with secondary anti-rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate, Cell Signaling). Stained cells were then washed twice with PBST and resuspended in 300 μL of PBS with 5 μg/mL DAPI to stain cells for DNA content.
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa 555-Goat anti-Mouse IgG (H+L) Secondary Antibodies were used at 1:500-1:1000 for IF (Cell Signaling Technology). DyLight 680-Goat anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated with anti-rabbit Alexa Fluor 488 and anti-mouse IgG (H+L) Fab2 Fragments Alexa Fluor 594 (Cell Signaling, 8890S), washed ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were probed with the appropriate concentrations of primary antibodies against cathepsin D (1:100; Cell Signaling Technology, 2284), GAPDH (1:200 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1β (30 ng/ml, Cell Signaling Technology, 8900SF) and TNF-α (30 ng/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... (TTBS) for 1 h at ambient and stained using a Phospho-Akt (Ser473) (D9E) and Histone H3 (D1H2) (all Cell Signaling Technologies, UK) diluted 1:1000 in 5% BSA in TTBS ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular phospho-stainings were performed 1 h at room temperature in the dark with antibodies against phospho-STAT5 (Tyr694; D47E7 XP Rabbit mAb #4322; Cell Signaling, 1:150), phospho-S6 ribosomal protein (Ser235/236 ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated (for 1 h) at room temperature with SignalStain® Boost Detection Reagent (HRP, cat. no. #8114; Cell Signaling Technologies, Inc.). Sections were washed three times with wash buffer and developed with freshly prepared and filtered 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were subsequently blocked with 5% milk or 5% bovine serum albumin for 1 h at room temperature and probed separately with specific primary antibodies to AKT1 (1:1,000 dilution, 75692, Cell Signaling Technology, Danvers, MA), pan-AKT (1:1,000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS−/−] at room temperature for 1.5 h and subsequently incubated with primary rabbit anti-MPO antibody (Cell Signaling, clone: E1E7I, 1:500) and primary mouse anti-HLA-G antibody (BD ...
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bioRxiv - Genetics 2022Quote: ... Neurons were subsequently blocked with 5% normal goat serum in 1x PBS for 1 h and then incubated with primary antibodies as follows: anti-GFP (Cell signaling, #2555S, R), anti-Gephyrin (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... Paraffin sections were used for H&E staining as well as sequential double-immuno-staining with rabbit anti-human Ki67 (Cell Signaling Technologies, US) and rabbit anti-human vimentin (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... The PVDF membrane was incubated with 5% w/v non-fat dry milk or 5% BSA for 1 h at room temperature and immunoblotted with anti-HSL (phospho-serine 660) (1:1000, Cell Signaling Technology, 45804S), anti-HSL (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000 ...
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bioRxiv - Genomics 2022Quote: ... Cleaved Caspase1 and cleaved Gasdermin D antibodies were purchased as a part of Pyroptosis Antibody Sampler Kit (#43811T, Cell Signaling Technology). After overnight primary incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were blocked with 3% BSA in PBS-T for 30 min and then incubated for 1 h with the following primary antibodies (diluted in 1% BSA in PBS-T): anti-RPA (Cell Signaling, #2208, 1:200), anti-Rad51 (Santa Cruz ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated for 1.5 h at RT with either CD36 (Novus NB400-144, 1:500 dilution) or PPARγ (Cell Signaling 2430S, 1:250 dilution) and GAPDH (Abcam ab181602 ...
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bioRxiv - Cell Biology 2020Quote: SMC were seeded at a density of 75000/well in 6 well plates and cultured in FGM for 48 h prior to fixation and staining using a commercial β-galactosidase assay (Cell Signaling Technologies #9860) as described previously (41) ...
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bioRxiv - Cell Biology 2020Quote: ... was performed for 24 h and cells were subject to immunofluorescence staining with an anti-BrdU antibody (1:400 dilution, Cell Signaling, cat no. 5292) before counterstained with DAPI and assessed by fluorescence microscopy.
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bioRxiv - Cell Biology 2021Quote: ... was performed for 16-18 h and cells were subject to immunofluorescence staining with an anti-BrdU antibody (1:400 dilution, Cell Signaling, cat no. 5292) before counterstained with DAPI and assessed by immunofluorescence and high content analysis microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The membrane was washed four times in 0.1% TBST buffer and incubated for 1 h at room temperature with anti-rabbit IgG conjugated to HRP-linked antibody #7074 (Cell Signaling Technology, Danvers, MA) diluted 1:5,000 with 0.05% TBST containing 5% skimmed milk ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 5 μM thick paraffin-embedded liver sections were used for hematoxylin and eosin (H&E) staining and Ki67 (Cell signaling technology 12202S, 1:400) immunohistochemical staining ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS-T and they were incubated with goat anti-rabbit IgG H&L Alexa Fluor 488 (Cell Signaling Technology, MA, USA) for 90 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibodies used were Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5151 and Anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5257 (Cell Signaling Technology) respectively ...
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bioRxiv - Systems Biology 2024Quote: ... Membranes were blocked in 5% milk or bovine serum albumin (BSA) for 1 h at room temperature and incubated with primary antibodies against: PI3 Kinase Class II α (Cell Signaling, 1:2000), Phospho-AKT(Ser473 ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed 3x5 min in TBST and incubated for 1 h with HRP-conjugated anti-rabbit IgG (Cell Signaling Technology, Cat No. 7074), HRP-conjugated anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked with 5% BSA for 1 h at room temperature and incubated overnight at 4ºC with primary antibodies targeting phopsho-TBK1 (Cell Signaling, 5483, 1:1,000 dilution), TBK1 (Cell Signaling ...