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Citations for Cell Signaling Technology :
151 - 200 of 653 citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872) and homogenized with a Teflon-glass homogenizer ...
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bioRxiv - Physiology 2019Quote: ... homogenized in CER-I buffer containing a protease/phosphatase inhibitor cocktail (Cell Signaling Technology [CST], 5872) and centrifuged at ∼16,000 g ...
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bioRxiv - Neuroscience 2019Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872). The retinas were then homogenized and incubated on ice for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% SDS) with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872) and homogenized with a Teflon-glass homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... 1-mM CaCL2 in H2 O) supplemented with 1:100 protease/phosphatase inhibitor cocktail (Cell Signaling). The protein content was measured using a Pierce BCA assay kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples were collected and lysed using RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC-1 cells were lysed in buffer containing protease and phosphatase inhibitors (Cell Signaling, catalog number: 9803S). Total protein content was quantified by Bradford assay and equal amounts of proteins were analyzed in duplicate with arrays containing 4 to 6 spots for each of 60 probes (ACC058 ...
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bioRxiv - Cancer Biology 2020Quote: ... NP-40 buffer was supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA, Cat# 5872S) and used to lyse frozen pellets ...
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bioRxiv - Pathology 2021Quote: ... Tendons were homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease/phosphatase inhibitors (Cell Signaling Technology [CST] #58725) using 0.5-mm zirconium oxide beads and a Bullet Blender Gold Cell Disrupter (Next Advance Inc.) ...
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bioRxiv - Molecular Biology 2022Quote: ... and associated proteins were harvested from top layer with RIPA buffer containing phosphatase inhibitor cocktail (Cell Signaling), and protease inhibitor cocktail (Sigma).
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bioRxiv - Genomics 2019Quote: Protein lysates were obtained using ice-cold 1X RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling 9806), and freshly supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology 5872S) and 100U/mL Benzonase (Sigma-Aldrich E1014) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with Protease and Phosphatase Inhibitor for assessment of total Stat 1(Cell Signaling Technology, Danvers, Massachusetts, USA) as well as phosphorylated-Stat 1 (Cell Signaling Technology ...
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bioRxiv - Physiology 2023Quote: ... using 20 μl/mg RIPA buffer supplemented with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology, MA, USA). The homogenized tissue was centrifuged at 14,000rpm at 4°C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: The EV pellet was resuspended in RIPA buffer with protease/phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA). Protein (20 µg ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared using RIPA buffer containing cOmplete ULTRA protease and phosphatase inhibitor (Cell Signaling Technology (CST), Danvers ...
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bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 10% sucrose, 0.8 M NaCl, 1 mM EDTA, 0.1% sarkosyl and 1X protease/phosphatase inhibitor cocktail, #5872, Cell signaling) in a 15 ml glass homogenizer with 15 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue (n=3 tendon per sample) was homogenized in RIPA buffer (including 1X protease/phosphatase inhibitor, Cell Signaling #58725) to extract the total protein with the Biomasher II system and Powermasher II electric stirrer (Nippi Inc ...
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bioRxiv - Cell Biology 2023Quote: ... then cells were lysed in 100-200 μL RIPA buffer containing 1X protease-phosphatase inhibitor cocktail (Cell Signaling, #5872S), as previously described [95] ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872S) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014).
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bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v SDS and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014).
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bioRxiv - Neuroscience 2024Quote: ... CSF samples were measured using a pipette then supplemented with 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technologies, #5872). CSF was stored at -80℃.
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bioRxiv - Microbiology 2019Quote: ... The inhibition of Akt activity was confirmed by western blot analysis with rabbit antibody against phospho-S473-Akt (#9271, Cell Signaling Technology), rabbit antibody against Akt (pan ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Immunology 2020Quote: ... cells were cytospun 5’ at 500 rpm and senescence-associated beta-glalactosidase activity measured as recommended by the manufacturer (Cell Signaling Technology) on a Zeiss-Axio inverted phase-contrast microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed once with 0.5% BSA/PBS and once with PBS prior to be resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0, and 1% SDS) supplemented with protease/phosphatase inhibitor (Cell Signaling), and chromatin was solubilized by sonication in a Bioruptor (Diagenode ...
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bioRxiv - Cell Biology 2022Quote: ... isolated rat cardiomyocytes were lysed in RIPA buffer (Cayman #10010263) supplemented with protease and phosphatase Inhibitor cocktail (Cell Signaling #5872S) on ice for 1 hour ...
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bioRxiv - Systems Biology 2020Quote: To probe signaling activity we used the following antibodies: anti-Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb (Cell signaling technology #9624S, 1:2000), anti-Phospho-CREB (Ser133 ...
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bioRxiv - Immunology 2021Quote: The primary CD4+ T cells were lysed in the RIPA buffer with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA). After determining the protein concentration for each cell lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed by ice-cold RIPA buffer (150mM NaCl, 1% NP40, 0.1% SDS and 50mM Tris, PH7.4) containing protease and phosphatase inhibitor (Cell Signaling Technologies; 5872) for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Flash frozen liver chunks were weighed and lysed with RIPA buffer (Astral Scientific, Australia) containing protease and phosphatase inhibitors cocktail (Cell Signaling Technology) at a concentration of 10 μL of RIPA per mg of tissue ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lyzed by addition of lysis buffer with Protease Inhibitor Cocktail III (Bioprocessing Biochemical, California) and Phosphatase Inhibitor Cocktail (Cell Signaling Technology). Equal amount of protein (micro-BCA method ...
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bioRxiv - Systems Biology 2019Quote: ... washed with 1 mL HBSS and lysed in 100 μL of 9 M urea containing protease and phosphatase inhibitor cocktail (Cell Signaling Technology). Samples were split into two 30 μg aliquots and separated on a Bolt 4-12% Bis-Tris Plus SDS gel (Cat ...
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with ice-cold PBS and lysed in 100 µL of phosphatase and protease inhibitor containing RIPA buffer (Cell Signaling Technology). Cells were incubated on ice for 30 min and then harvested by scraping followed by centrifugation at 14,000 rpm for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM of the prolyl hydroxylase inhibitor DMOG and 10 µM of the protease/phosphatase inhibitor MG-132 (Cell signaling; product 5872S) were used to prevent degradation of HIF ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% w/v sodium dodecyl sulfate (SDS) and 90mM NaCl) supplemented with 1x Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology Cat#5872) and 100 U/mL Benzonase (Sigma-Aldrich Cat#E1014) ...
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Cancer Biology 2020Quote: For WCM154 Western blots, cell pellets were lysed in RIPA buffer (MilliporeSigma, 20-188) supplemented with Protease/Phosphatase Inhibitor Cocktail (Cell Signaling Technology, 5872S). Protein concentrations were assayed with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: Western blotting was performed using a routine protocol after cell lysis in RIPA buffer including protease and phosphatase inhibitors (#9806 and #5872 Cell signaling, Frankfurt, Germany). Grayscale intensity values were normalized to internal positive controls and measured using ImageJ software (NIH ...
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bioRxiv - Genetics 2023Quote: MEFs were washed twice with cold 1x PBS and lysed using RIPA buffer with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology 5872) to obtain total protein samples ...
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bioRxiv - Neuroscience 2023Quote: ... cultured primary pericytes were lysed with cold lysis buffer of radioimmunoprecipitation assay (RIPA) lysing buffer supplemented with 1x protease/phosphatase inhibitor (Cell Signaling Technology; U.S.A). Cells were mechanically agitated for 5 minutes and incubated on ice for a further 30 minutes with occasional agitation ...
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bioRxiv - Developmental Biology 2019Quote: ... proteins were extracted from the heads of 30-50 wildtype and mdkami5001 embryos in cold RIPA lysis buffer containing protease and phosphatase inhibitor cocktail (Cell Signaling Tec., Danvers, MA). Proteins were separated in 12%Mini-PROTEIN TGX Precast gel (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: Tissue was collected and incubated on ice for 60 minutes using RIPA buffer containing 1X RIPA including a phosphatase inhibitor Na3VO4 (Cell Signaling, Leiden, the Netherlands), 1 mM phenylmethylsulfonyl fluoride (PMFS ...
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bioRxiv - Cancer Biology 2024Quote: Total protein was extracted from spheroids and organoids using lysis buffer supplemented with a protease/phosphatase inhibitor cocktail (both from Cell Signaling Technology, MA, USA). Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and liver organ samples taken from 12-month-old mice were lysed in ice-cold RIPA buffer supplemented with Protease & Phosphatase Inhibitor Cocktail (Cell Signaling Technology 5872 S, 50 mg/ml) and Benzonase (Sigma-Aldrich E1014 ...
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bioRxiv - Cancer Biology 2022Quote: ... Kit (Cell Signaling Technology, 3074), Ido1 (Santa Cruz Biotechnology ...