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Citations for Cell Signaling Technology :
151 - 200 of 207 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 6 diamidino-2-phenylindole (DAPI, 0.5 μg/mL in PBS, Cell Signaling Technology). Stained sections were mounted with ProLong® Gold Antifade Reagent (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2021Quote: ... Animals were labeled with 1:800 anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies), BODIPY FL-Phallacidin ...
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bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescent stainings were performed as previously described (8) with GR (Cell Signaling, 12041, 0.5μg/ml), Ki-67 (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Animals were labeled with 1:400 anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies), BODIPY FL-Phallacidin ...
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bioRxiv - Neuroscience 2021Quote: ... 50 mM β-glycerophosphate) and 6 μL of anti-pS6 antibody (Cell Signaling D68F8) were added ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; Cell Signaling Technology, USA), and finally samples were mounted with ProLong® Gold Antifade Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Rabbit monoclonal antibodies against 6-His-tags (12698S) were purchased from Cell Signaling Technology. The rabbit monoclonal anti-histone H2A ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-NeuN (6 ug/brain sample; Cell Signaling Technology, Danvers, MA; #24307) antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... stained overnight using primary antibodies 1:200 VE-Cadherin (Cell Signaling; D87F2; Lot: 6) or 1:200 Fibroblasts Antibody TE-7 (Novus Biologicals ...
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bioRxiv - Immunology 2023Quote: ... These tissue samples were washed extensively (6 times) with RBC lysis buffer (Cell Signaling Technology) until the soret absorption peak on the UV-visible spectra was almost negligible and the washes were colorless ...
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bioRxiv - Pathology 2020Quote: ... Fatty acid binding protein (FABP)-4-specific rabbit monoclonal antibody (1:1000 dilution, D25B3, Cell Signaling), BNIP3 specific rabbit monoclonal antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Following nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA), larvae were mounted in 80% glycerol between two coverslips and imaged using fluorescence confocal microscopy ...
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bioRxiv - Genomics 2022Quote: ... H3K9me3 immunoprecipitation was performed on 3 million cells with 8 µL of H3K9me3 antibody (Cell Signaling, 13969S Lot #3). Samples were sequenced using a NovaSeq SP kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for staining: anti-SOX2 (rabbit, Cell Signaling Technology, 3579S, 8, 1:300 dilution), anti-MAP2 (guinea pig ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by incubation with primary Antibody against pSMAD1/5/8 (the antibody was purchased from Cell Signaling Technology; no. 9511) at 1:200 dilution in blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... BMP signaling was measured using a monoclonal phospho-SMAD 1/5/9(8) antibody (Cell Signaling Technology, Danvers, MA, #13820) at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
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bioRxiv - Cell Biology 2021Quote: Cells cultured on 6-well plates were stained using SA-β-gal activity assay kit (Cell Signaling Technology) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with syntaxin-6 antibody (C34B2) Rabbit mAb 2869 (Cell Signaling Technology Europe B.V, 2869S; 1:100) for 16Lh at 25L°C with gentle agitation.
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bioRxiv - Cell Biology 2024Quote: ... tissues were coverslipped with Prolong Gold Antifade Mountant containing 4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling, 8961).
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg total protein was resolved in 8% non-reducing SDS-PAGE gel and blot with PKM2 antibody (Cell Signaling, 3198s).
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bioRxiv - Neuroscience 2020Quote: ... 50 mM NaCl (pH 8) with a protease inhibitor (phenylmethylsulfonylfluoride, 0.5 mM final concentration and protease inhibitor cocktail from Cell Signaling Technology). Cells were lysed by sonication on ice for 10 min (20 s on ...
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bioRxiv - Genetics 2020Quote: ... Cells were briefly centrifuged to the bottom of the wells and incubated for 8 min (for VSMCs) or 15 min (for THP-1 cells) before lysis with cell lysis buffer (#9803, Cell Signaling Technologies) containing a cocktail of protease and phosphatase inhibitors ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with primary anti-RAB5 antibody at 4 °C overnight diluted in 8% BSA in PBST (1:500 dilution, Cat. #3547, lot 7, Cell Signaling Technology). Secondary anti-rabbit Alexa Fluor 568 antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 8 min at 37 °C with primary antibodies against HA (rabbit, 1:250, Cell signaling Technology, cat #C29F4). After washes ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies used for experiments include eGFP (Living Colors A.v. Monoclonal Antibody JL-8; catalog # 632381 and anti-mouse HRP-linked secondary antibody (Cell Signaling; catalog #7076).
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bioRxiv - Cancer Biology 2021Quote: G6PD enzyme activity was determined using the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit (12581S, Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear staining was carried out by using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Larvae were imaged using fluorescence confocal microscopy.
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was carried out by using 4’,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Four different sections with no tissue loss were counted for the quantification of cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 uL of the sample were mixed with 8 uL of 1x PBS and were lysed in 1x radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, 9806) for 30 minutes at RT ...
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bioRxiv - Biochemistry 2020Quote: ... at 1:1,000 dilution in blocking buffer or glucose 6-phosphate dehydrogenase (G6PDH) antibodies (8866, Cell Signaling Technology, 1:1,000). Immunoreactive species were detected with antibodies conjugated with horseradish peroxidase (715-035-150 ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were sacrificed 6 days after noise exposure and whole cochlear lysates were prepared in lysis buffer (9803; Cell Signaling) after adding protease (cOmplete ULTRA Tablets 05892791001 ...
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bioRxiv - Cancer Biology 2022Quote: ... their nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope (Carl Zeiss AG ...
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bioRxiv - Cancer Biology 2022Quote: Senescence was assessed using the Senescence β-Galactosidase Staining Kit at pH 6 following the manufacturer’s instructions (Cell Signaling Technology). For SAHF staining ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated with 25 μg/mL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride; Cell Signaling Technology, USA) for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest was split into 8 aliquots dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784) as described in the manual and peptides were eluted twice with 200µL of 50% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Physiology 2022Quote: ... Isolated islets and MIN-6 cells were mechanically homogenized in ice cold RIPA lysis buffer (Cell Signaling Technology, Danvers, MA, USA) supplemented with 1x protease inhibitors cocktail (Sigma ...
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bioRxiv - Physiology 2023Quote: Expression of specific proteins in polysomal profiles (Fig. 6) was determined by probing the membrane with antibodies against RsL7a (dilution 1:4000) from Cell Signaling; 4EBP (dilution 1:4000) ...
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bioRxiv - Genomics 2022Quote: ... acid-extracted histone lysate was prepared accordingly (Shechter et al., 2007) and subjected to western blotting using anti-H3K9ac (Cell Signaling, 9649), H3K14ac (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... m-h-anti-CHOP 1:1000, m-h-anti-fatty acid synthase 1:100, cat#3180T; m-h-anti-vinculin 1:1000, cat#13901S; Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: MCF7 cells were grown in 6 well plates for Western blots and were washed with cold PBS twice and scraped in RIPA buffer (Cell signaling, USA). Protein concentration in each sample was measured through Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... and Samples were washed extensively in PBS-Tx (6 × 1 hour) and incubated with anti-rabbit-HRP (Cell Signaling #7074, 1:1000) diluted in 1% BSA/PBS-Tx ...
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bioRxiv - Cell Biology 2021Quote: The staining of SA-β galactosidase in cells at pH-6 was performed using a senescence β-Galactosidase staining kit according to the instructions of the manufacturer (Cell signaling). Measurement of the absorbance at 420 nm from SA-β galactosidase activity detectable at pH 6.0 was performed as described (Lee et al. ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed on 6% acrylamide SDS-PAGE gels followed by immunoblotting with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S). In vitro dephosphorylation experiments were performed in three biological replicates.
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bioRxiv - Neuroscience 2022Quote: ... To detect changes in IL-6 signal transduction we used primary monoclonal rabbit anti-Stat3α (D1A5) from Cell Signaling Technologies (Cat no. 8768S) and used the same secondary anti-rabbit antibodies described above.