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Citations for Cell Signaling Technology :
1901 - 1950 of 2108 citations for TNF alpha TNFSF2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were incubated with primary antibodies overnight at 40C and followed with the relevant HRP-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody cell signaling-7074 and Anti-mouse IgG, HRP-linked Antibody Cell signaling-7076) and visualized using Clarity Western ECL Substrate (BIORAD ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed and incubated in blocking buffer containing HRP-linked horse anti-mouse (1:5000; Cell Signaling Technology Cat#7076) for 45 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... Samples were then incubated in a mixture of Alexafluor 488-labelled anti-rabbit IgG and Alexafluor 532-labelled anti-mouse IgG (both Cell Signaling Technology) (dilutions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with appropriate secondary antibodies for 1h: Alexa Fluor 488 or 555 or 647 Goat anti-Mouse IgG (2 μg/mL, Cell Signaling Technology). Cells were then permeabilized as previously described (Souriant et al. ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Cancer Biology 2019Quote: ... Dilution 1:5000) and anti-mouse (Cat no.: 7076; Dilution 1:5000) secondary antibodies (1 μg/μl) purchased from Cell Signaling Technology were used to detect primary antibodies in immunoblotting studies.
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bioRxiv - Physiology 2021Quote: ... A mouse primary anti-CaSR monoclonal antibody (1:2000, Gentex, Zeeland, MI) or a mouse primary anti-Myc (9B11) monoclonal antibody (1:1000, Cell Signaling Technology) was applied followed by a secondary horseradish peroxidase-coupled secondary antibody (1:5000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Both Anti-ERVK-env mouse monoclonal (HERM-1811-5, Austral Biologicals, San Ramon, CA, USA) and anti-CDH1 rabbit monoclonal (Cell Signaling, 3195S) antibody were diluted 1:250 in PBS + 1% BSA ...
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bioRxiv - Genetics 2021Quote: ... Specific proteins were detected using 1:1000 dilution of Myc-Tag (9B11) mouse monoclonal and proliferating cell nuclear antigen (PCNA, D3H8P) XP Rabbit monoclonal primary antibodies (Cell Signaling Technology). Following primary antibody incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the following antibodies were used anti-Bassoon (1:400, SAP7F407; mouse monoclonal; Enzo Life) and anti α-synuclein (1:500, D37A6; rabbit polyclonal; Cell Signaling). Species-specific secondary antibodies coupled to Alexa 488 and 350 (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK1/2 (#9102, Rabbit, WB 1:1000) and MEK1/2 (#4694, Mouse, WB 1:1000) antibodies were purchased from Cell Signaling Technology, MA ...
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... and immunoblotted for ubiquitin as per standard protocols (anti-ubiquitin antibody P4G7, BioLegend #838701, 1:1000; anti-mouse IgG HRP-linked antibody, Cell Signaling Technology #7076 ...
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bioRxiv - Immunology 2020Quote: ... Blots were then incubated in HRP-linked secondary antibody against mouse or rabbit IgG (1:3000, Cell Signaling, Cat# 7074S and 7076S) in 4% BSA for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoblots were then incubated with secondary antibody for 1 h at room temperature (1:1000 dilution, anti-rabbit Cat. #7074, lot 28, or anti-mouse HRP-conjugate Cat. #7076S, lot 32, Cell Signaling Technology). Specific signal was detected using SuperSignal West Pico Chemiluminescent Substrate (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed with Tween-TBS and incubated in secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076; both Cell Signaling technology) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... primary antibody pairs against NFκB total (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse-anti-NFκB p65 total, Cell Signaling Technology #6956); NFκB phospho (rabbit-anti-NFκB p65 total ...
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bioRxiv - Immunology 2022Quote: ... The following horseradish peroxidase (HRP) conjugated secondary antibodies were used for this study: mouse anti-rabbit IgG (L27A9) monoclonal antibody (Cell Signaling #5127), goat anti-rabbit IgG polyclonal antibody (Cell Signaling #7074S) ...
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bioRxiv - Physiology 2023Quote: ... Bound primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse or -rabbit secondary antibodies (Cell Signaling Technology, Inc., Danvers, MA), and protein signals were visualized using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100uM glycine for 30 minutes at room temperature and labeled overnight at 4C with primary antibodies (rabbit anti-FOXP2 Abcam #16046 1:500, rat anti-Flag 1:500 Novus NBP1-06712, mouse anti-NeuN Ab 1:500, Cell Signaling #94403S). For secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Rabbit Secondary Antibody in 1:30000 dilution and Anti-Mouse Secondary Antibody (Cell Signaling Technology, 5151; Li-Cor, 926-68020, respectively) were used in 1:15000 dilution during Western Blotting experiments.
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bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat. 901514; rabbit, Cell Signaling 3724S), and two PLA probes (anti-rabbit-PLUS ...
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bioRxiv - Biochemistry 2022Quote: ... The blots were subsequently incubated overnight at 4°C with primary antibodies against p53 (DO-7 mouse mAb, Cell Signaling, Cat # 48818) and β actin (13E5 rabbit mAb ...
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bioRxiv - Microbiology 2023Quote: ... with HRP-conjugated secondary antibodies (Goat Anti-Rabbit Immunoglobulins/HRP, Dako P0448; Horse Anti-Mouse IgG, HRP-linked, Cell Signaling Technology #7076). BCIP/NBT substrate was used to detect ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed in TBST and then incubated for 45 min at room temperature in blocking buffer containing either anti-rabbit or anti-mouse HRP-linked secondary antibody (Cell Signaling Technology). Membranes were washed in TBST and imaged on the ChemiDoc Imaging System using SuperSignal chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... followed by 1 h incubation in horseradish peroxidase–conjugated secondary antibody (anti-rabbit or anti-mouse, #7074 and #7076, Cell Signaling Technology) diluted in PBS-T 5% milk at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... over night at 4 °C with a dilution of 1:200 and subsequent incubation with a HRP-linked secondary anti-mouse antibody (Cell Signaling #7076) for 2h at RT with a 1:5000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2, 1:1000, Cell Signaling; rabbit anti-AKT3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Biochemistry 2023Quote: ... the fixed cells were blocked in PBS containing 5% bovine serum albumin (BSA) and then incubated with mouse anti-HA antibody (6E2, Cell signaling Technology) with 1:800 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-α-tubulin DM1α conjugated to Alexa Fluor 488 (1:100, Cell Signaling Technologies 8058S), mouse anti-centrin clone 20H5 (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell lysates were analysed by western blot with anti-Gli1 (L42B10) mouse monoclonal antibody (1:800, 2643, Cell Signaling Technology, RRID: AB_2294746) followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding secondary antibodies conjugated with horseradish peroxidase (cat no: 7074 Rabbit, cat no: 7076 Mouse, cat no: 7077 Rat, Cell Signaling Technology) in combination with enhanced chemiluminescence reagent (cat no ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against mouse anti-HA clone 6E2 (Cell Signaling Technology) or rabbit anti-TgSKP1 UOK75 [19].
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bioRxiv - Biochemistry 2023Quote: ... The blots were then incubated with a peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody (1:2000) (# 7074 or #7076, respectively; Cell Signaling Technology). The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... neurons and mouse brain tissues were lysed for 30 minutes on ice in appropriate volume of cold RIPA lysis buffer (Cell Signaling Technologies) supplemented with protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... the blots were washed with TBS-T(x3) and incubated with a secondary peroxidaseLconjugated antirabbit or mouse IgG antibody (1:2000) (#7074 or #7076, Cell Signaling Technology) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for WB: Anti-mouse IgG HRP antibody (Cell Signaling Technology #7076S, 1:10000) and anti-rabbit IgG HRP (Cell Signaling Technology #7074S ...
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bioRxiv - Neuroscience 2024Quote: ... and detected using Goat anti-mouse Alexa Flour 647 and astrocytes were labelled with GFAP Alexa Flour 555 (3656S, Cell Signaling Technology). Slides were mounted with Prolong Diamond (P36961 ...
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bioRxiv - Immunology 2023Quote: ... Rabbit monoclonal anti-FLAG (D6W5B) antibodies (Cat # 14793S) and Mouse monoclonal anti-MYC (9B11) antibodies (Cat# 2276S) were obtained from Cell Signaling Technology. Mouse polyclonal anti-V5 antibodies were purchased from MBL Life Science (Cat# PM003) ...
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bioRxiv - Immunology 2023Quote: ... The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100, 78060S, Cell Signaling Technology) in Ce3D™ Antibody Diluent Buffer (48 hr ...