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Citations for Cell Signaling Technology :
1901 - 1950 of 2288 citations for Recombinant Mouse Cd27 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The following primary antibodies were used: mouse anti-phospho-myosin light chain 2 (S19) (Cell Signaling 3675S, 1:100 dilution), mouse anti-E-cadherin (BD Biosciences 610181 ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... Slides were then incubated at 4°C overnight with the corresponding primary antibody (anti-acetylated tubulin, Sigma T7451, mouse monoclonal, 1/60,000 dilution; anti-cleaved caspase 3, Cell Signaling 9579 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-GAPDH, 1.10000 (10R-G109a, Fitzgerald Industries international, Acton, MA; rabbit anti-β-tubulin, 1.1000 (#2146, Cell Signaling Technology); rabbit anti-histone H2B ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated for 1h with a secondary antibody (anti-mouse HRP conjugate at 1:10,000 dilution -Cell Signaling) and chemiluminescent substrate ultra (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... and horseradish peroxidase (HRP)-labeled anti-mouse IgG (H + L) (cat #, 7076S, 1: 2,000, Cell Signaling Technology, Danvers, MA, USA) were used as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-ý-Actin (Millipore Sigma, mouse mAb, 1:5000 or Cell Signaling Technology, rabbit mAb, 1:5000, as indicated). The secondary antibodies used were anti-rabbit HRP-linked (CST 7074 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was then incubated for 1 hour at room temperature with either DyLight 800 anti-mouse (Cell Signaling, #5257) or DyLight 580 anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal GAPDH (Thermo ScientificTM, Cat: MA5-15738) and rabbit α-TUBULIN (Cell Signaling Technologies™, Cat: 50-190-297) were used as loading controls in immunoblot assays.
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bioRxiv - Biophysics 2023Quote: ... The membrane was washed 3 times for 5 min in TBST (20 mM Tris pH 7.6, 150 mM NaCl, 0.1% Tween20) before incubation with anti-mouse-HRP (Cell Signaling Technologies) at a 1:2000 dilution in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected by incubating blots for 1h in secondary anti-mouse HRP conjugate diluted 1:6,000 or secondary anti-rabbit HRP conjugate (Cell signaling) diluted in milk blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated with Horse Raddish Peroxidase (HRP)-conjugated goat anti-mouse IgG secondary antibody (Cell Signaling, cat. #7076) at 1:20000 (to detect GluN2C or GluN2A ...
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bioRxiv - Neuroscience 2023Quote: ... and goat anti-mouse IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 488 (Cell Signaling Technology, #4408). Each well ...
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bioRxiv - Genetics 2023Quote: ... Brain sections were incubated with primary antibodies overnight at 4°C (mouse-NeuN, 1:500, abcam ab177487; rabbit-Cas9, 1:1,000, Cell Signaling clone D8Y4K ...
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bioRxiv - Pathology 2023Quote: ... Mouse lung tissue sections were stained with anti-CD31 antibody (77699, Cell Signaling Technology, Danvers, MA, USA 1:200 dilution), anti-TrkB antibody (AF1494 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... HRP-linked rabbit IgG (cat#: 7074) and HRP-linked mouse IgG (cat#: 7076) antibodies were purchased from Cell Signaling Technology. The anti-FLAG HRP antibody (clone M2 ...
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bioRxiv - Neuroscience 2023Quote: Cryosections of P0 mouse brains were immunolabeled with cleaved-caspase 3 (c-cas 3) antibody (Cell Signaling #9961, 1:4000) for apoptosis analysis ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min three times and then incubated with the appropriate secondary antibody (anti-mouse #7076 or anti-rabbit #7074 (both 1:1,000; Cell Signaling) or anti-goat (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were incubated with 0.5 ml PBS BSA 3% (A1391, Applichem) with primary antibodies (mouse-anti-HA monoclonal antibodies (C29F4, Cell Signaling) at 1:500 ...
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated in 4% milk supplemented with either anti-mouse IgG HRP-conjugated secondary antibody (Cell Signaling Technologies, 7076P2) (1:10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with peroxidase-conjugated anti-mouse IgG antibodies (1:5,000 dilution, 55558, Cappel) or anti-rabbit IgG antibodies (1:5,000 dilution, 7074, Cell Signaling Technology). The membranes were then reacted with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed with TBS-T and incubated at RT for 2 h with horseradish peroxidase-labeled secondary antibody (1:3,000, anti- rabbit #7074, anti-mouse #7076 or anti-rat #7077, Cell Signaling) diluted in a solution of 5% milk in TBS-T ...
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bioRxiv - Microbiology 2024Quote: ... Blots were then incubated with anti-rabbit IgG or anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibodies (1:10,000; Cell Signaling) in TBST-milk for 1-2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... (dilution 1:1,000) and HRP-conjugated anti-Mouse (dilution 1:10,000) and antiRabbit (dilution, 1:10,000) antibodies from Cell Signaling Technology (MA, USA). Bcl-2 (C-2 ...
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bioRxiv - Biochemistry 2020Quote: ... BSA was removed with PBS washes and primary antibodies detecting IdU and a protein of interest were diluted in 1% BSA/0.3% TritonX-100 and added to cells overnight at 4°C (IdU: mouse anti-BrdU, 1:20, Becton Dickinson; Total Histone H4: rabbit anti-Total Histone H4, 1:500, Upstate Cell Signaling Solutions 05-858 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were rinsed and incubated with Horseradish Peroxidase (HRP) conjugated anti-rabbit (1:5000) and anti-mouse (1:5000) secondary antibodies (Cell Signaling) for the rapamycin blot or with the fluorescent StarBright Blue 700 anti-mouse (1:5000 ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) overnight ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with either a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) or 1:10,000 anti-rabbit secondary antibody (Cell Signaling ...
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bioRxiv - Molecular Biology 2021Quote: ... monoclonal antibodies followed by the by the anti-mouse IgG Alexa fluor 488 or anti-rabbit IgG Alexa fuor 555 secondary antibody (Cell Signaling). Then samples were re-fixed in 2% formaldehyde ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-MYC tag (#2276, 1:200 for IF), rabbit anti-cleaved-Dcp-1 (#9578, 1:200) from Cell Signaling Technology, mouse anti-GFP (MAB3580 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were rinsed and incubated with horseradish peroxidase conjugated secondary antibody (Anti-mouse IgG, HRP-linked Antibody (Cell Signaling Technology, 7076), Anti-rabbit IgG ...
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bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... The dilutions of HRP-conjugated secondary antibodies were 1:2000 for goat-anti-guinea pig IgG and 1:2000 for anti-mouse IgG (Cell Signaling). Signal Fire ECL substrates (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in 8 well chamber slides and fixed in 4% paraformaldehyde and permeabilized using 0.1% saponin in blocking buffer (HBSS containing 3% BSA, 0.2% gelatin, and 0.02% NaN3) and stained with Rabbit anti-mouse YAP1 (Cell Signaling Technology, D8H1X), FITC-conjugated phalloidin ...
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bioRxiv - Microbiology 2020Quote: ... After this anti-rabbit IgG secondary antibody (for Sap and EA1) or anti-mouse IgG secondary antibody (for BslO) conjugated with horseradish peroxide (1:10,000 - Cell Signaling Technology) was used and the blots were incubated for another 60 min ...
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bioRxiv - Neuroscience 2019Quote: ... Then the slices were incubated for 24 h at 4°C with primary antibodies, anti-mouse TH (dilution 1:1000, M318, millipore) and anti-rabbit c-Fos (dilution 1:200, 2250, Cell Signaling). The secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in TBST for primary or 3% BSA in TBST for secondary) were used for immunofluorescence: E-cadherin (#14472, monoclonal mouse, 1:100, Cell Signaling), mouse-IgG Alexa Fluor 594-conjugated secondary (#8890 ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in 5% milk in TBST) were used for western blotting: E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), N-cadherin (#13116 ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-linked anti-mouse IgG or HRP-linked anti-rat IgG were used as secondary antibodies (Cell Signaling Technology, Danvers, MA). Membranes were imaged with ChemiDoc imaging system (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... Anti-mouse (7076) secondary antibody was used at a concentration of 1:10,000 in TBS-T and was from Cell Signaling Technology.
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bioRxiv - Biochemistry 2020Quote: ... Bound primary antibodies were detected with HRP-conjugated goat anti-mouse immunoglobulin-G at 1:5,000 (Cell Signaling Technoloy, Cat# 7076S) or goat anti-rabbit immunoglobulin-G at 1:2,500 (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2020Quote: ... and chemiluminescence protein detection was done as described with anti-mouse or anti-rabbit antibodies conjugated with horseradish peroxidase (HRP) from Cell Signaling Corp ...
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bioRxiv - Systems Biology 2020Quote: ... Membranes were then incubated for one hour at RT in either appropriate (mouse or rabbit) Horse radish peroxidase (HRP) - linked secondary antibodies (Cell Signaling) in TBST for chemiluminescent detection ...
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bioRxiv - Systems Biology 2020Quote: ... washed twice with ∼5 mL 0.1% BSA-PBS then resuspended cells in 0.16 mL 0.1% BSA-PBS containing 1:500 anti-mouse FAb2 secondary antibody conjugated to Alexa Fluor 488 (Cell Signaling #4408) and incubated on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit anti-Tubulin (cat# 2148), rabbit anti-NKX2.5 (clone E17AH, cat# 8792S), and mouse anti-SOX2 (clone L1D682, cat# 4900S) were from Cell Signaling Technologies. Mouse anti-OCT4 (cat# ab184665) ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse lung cryosections were stained overnight with anti-BrdU (1:3, BD Biosciences, or 1:1000 Cell Signaling Technology, Danvers, MA) and incubated with Alexa Fluo 488-conjugated secondary antibody (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then washed three times with TBS-T followed by incubation during 1 hr at room temperature with anti-rabbit or anti-mouse secondary antibody coupled to HRP (all from Cell signaling) diluted at 1:1,000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed in PBS-0,1% Tween20 three times for 15 min at room temperature and anti-rabbit or anti-mouse secondary antibodies (1:3000, Cell signaling) were applied for 30 min at room temperature with gentle agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in 5% milk protein and incubated with primary antibodies (Table S3) overnight followed by secondary HRP-linked goat anti-rabbit and anti-mouse (Cell Signaling) antibodies (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... one hour incubation in 0.05 % PBS-T supplemented with the secondary antibody (1:10,000 anti-mouse IgG, HRP-linked antibody, Art. No. 7076, Cell Signaling Technology® / 1:10,000 anti-rabbit IgG ...
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bioRxiv - Microbiology 2022Quote: ... Blots were incubated in primary antibody overnight and probed with 1:3,000 horseradish peroxidase (HRP)-conjugated horse anti-mouse (7075; Cell Signaling Technology) or goat anti-rabbit (7074 ...