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Citations for Cell Signaling Technology :
1901 - 1950 of 10000+ citations for Acetamide N 2 3 dihydro 2 3 hydroxy 2 quinolinyl 1 3 dioxo 1H inden 5 yl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were permeabilized with 0.1% Triton-X and stained for intracellular phospho-p44/42 Erk1/2 Thr202/Tyr204 at 1:700 dilution (Cell Signaling Technology, 4370) and p44/42 Erk1/2 at 1:700 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... Phosphorylated MAPKs were detected using α-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) rabbit monoclonal antibody (1:2000, Cell Signaling Technology, USA) for 1 h at room temperature in a solution of 5 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with the following primary antibodies: phospho-p44/42 MAPK (EER1/2) (T202/Y204) (Cell Signaling Technology, Cat. #: 9101, 1:1000), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... or phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (anti-phospho-TEY, #9101, 1:200, Cell Signaling Technology, Danvers, MA, USA). Membranes were washed 3 times for 5 min with TBST and incubated for 1 h with the appropriate secondary antibody – goat anti-rabbit (1:20,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-PKM 1/2 (C103A3, #3190), anti-PKM2 (D78A4, #4053), and anti-pY849 PDGFRα/pY857PDGFRβ (C43E9, #3170S) antibodies were purchased from Cell Signaling Technology. Rabbit monoclonal anti-GLUT1 (ab115730) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice in 1 ml FACS buffer and resuspended in 50-100 μl of 1:50 diluted primary PE-conjugated antibodies against phosphorylated p38 or Erk1/2 (Cell Signaling Technology) and stained for 1 h at room temperature on a rotor ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated rotating for 2 hr at 4°C with 1 uL anti-tri-methyl-histone H3 (Lys27) (C36B11, Cell Signaling Technology) or normal rabbit IgG (#2729 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... and incubated with primary antibodies at 1:1000 dilution (overnight at 4°C): rabbit polyclonal anti-phospho (Thr202/Tyr204) Erk1/2 (#9101; Cell Signaling Technology), rabbit polyclonal anti-Erk1/2 (#9102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was blotted with 1:1,000 dilutions of antibodies for phospho-p44/42 MAPK (phospho-Erk1/2; Cell Signaling Technology #9101), p44/42 MAPK (Erk1/2 ...
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bioRxiv - Genomics 2024Quote: ... Phospho-MAPK was detected using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (clone 4370, Cell Signaling, 1:100 dilution) by the UCSF Histology and Biomarkers Core on an automated Ventana BenchMark Ultra platform ...
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bioRxiv - Cancer Biology 2021Quote: ... Single clones were picked out after 3 weeks and Cas9 expression level in each clone was determined by western blotting with Cas9 antibody (Cell Signaling, #14697). Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and KC in the cell lysates and the culture supernatants were determined by western blot analysis using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control with specific antibodies (Cell Signaling Technology) as previously described (19).
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... and ɣH2AX (NB100-384) were from Novus; cleaved Caspase-3 (5A1E; 9664) and pS10 Histone H3 (9701) were from Cell Signaling Technology; GAPDH (6C5 ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Cancer Biology 2024Quote: ... Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology for immunoblot or fluorescence imaging analysis ...
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bioRxiv - Bioengineering 2024Quote: ... The antibodies against phospho-STAT3 (Tyr705, 9145), cleaved caspase-3 (Asp175, 9579) and PCNA (2586) were purchased from Cell Signaling Technology (USA). The antibodies against myeloperoxidase (ab208670 ...
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bioRxiv - Cell Biology 2024Quote: ... Crude cell extract was lysed with an insulin syringe and pre-cleared by incubation with 3 μg Normal Rabbit IgG (Cell Signaling Technology) and 10uL Pierce™ Protein A/G Magnetic Beads (Thermo Scientific 88803 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rabbit, pan, 40D4), phospho-AKT [rabbit, Ser473, (D9E)XP] and GAPDH (glyceraldehyde-3-phosphate dehydrogenasemouse, D4C6R) were purchased from Cell Signaling (USA). Antibodies against human PCNA (proliferating cell nuclear antigen ...
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bioRxiv - Physiology 2023Quote: Immunoblot experiments were performed as previously described (17, 18) using antibodies against cleaved caspase-3 (Cell Signaling Technology, Cat#9664, RRID: AB_2070042), procaspase-3 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Developmental Biology 2023Quote: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 45 min with 3 μg/ml Myc-Tag (9B11) Mouse mAb AlexaFluor 488 conjugate antibody (Cell Signaling Technology) and Phalloidin-Alexa 647 (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Cells were treated and prepared as described above for synchronization and flow cytometry with the addition of cleaved caspase-3 antibody (Cell Signaling Technology) conjugated to Alexa Fluor 647 added together with pH3 antibody ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then labeled overnight at 4℃ with primary antibodies for neurofilament-heavy (RT-97, Developmental Studies Hybridoma Bank, University of Iowa) or cleaved caspase-3 (Cell Signaling 9664S). After three rounds of washing ...
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bioRxiv - Cell Biology 2024Quote: ... The required primary antibody (JOSD1 [Abcam, Cambridge, United Kingdom], SOCS1 [Abclonal, Woburn, Massachusetts, United States of America], Cleaved Caspase 3 [Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit DA1E monoclonal antibody IgG isotype control (cat. # 3900) and cleaved caspase-3 rabbit monoclonal antibody (cat.# 9664) were all from Cell Signaling Technology. Anti-Iba1/AIF1 mouse monoclonal antibody (used for immunofluorescence in xenografts ...
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bioRxiv - Neuroscience 2020Quote: ... we used identical procedures except that we used LV-Syn-DIO-mTagBFP2-2A-GluN2B (1 × 109 TU/ml titer, 2 μL) and GluN2B antibody (#4207S, Cell Signaling, 1:1000, rabbit polyclonal antibody) (Supplementary Fig ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...