Labshake search
Citations for Cell Signaling Technology :
1851 - 1900 of 10000+ citations for 7 CHLORO 1 METHYL 1H PYRAZOLO 4 3 B PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... Flag (Sigma F1804; 1:1000 or Cell signaling #14793; 1:1000), b-actin (Cell signaling #4970 ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-Dcp-1 from rabbit (#9578, Cell signaling Technology, 1:200). Secondary antibodies used were purchased from Thermo Fisher Scientific and were as follows ...
-
bioRxiv - Neuroscience 2024Quote: ... and 647 (1:400, Life technologies; 1:1000, Cell Signaling Technology). After rinsing in PBS (three times) ...
-
bioRxiv - Cancer Biology 2024Quote: ... HSPD1 (Cell signaling, 4869S, 1:200 in 1% BSA/1X PBS) and cells were incubated overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... and incubated overnight at 4°C with the primary antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370S, Cell Signaling) at 1:2000 ...
-
bioRxiv - Immunology 2021Quote: ... Fixed and permeablized cells were then incubated at 4 ̊C with anti-phospho-S6 (Ser235/236) APC (clone D57.2.2E; Cell Signaling Technology #14733). Samples were acquired on a BD LSRII flow cytometer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney, and brain were homogenized at 4°C (Precellys Cryolys Evolution, Bertin Instruments) in lysis buffer (RIPA buffer, 1X, Cell Signaling). Tissue total protein concentration was measured by BCA assay (Pierce BCA) ...
-
bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
-
bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 h at RT in Odessy blocking buffer (Licor) and incubated overnight at 4°C with the following antibodies: IRF3 (Cell Signaling) 1:1000 ...
-
bioRxiv - Biochemistry 2019Quote: ... Sections were incubated overnight in a humid chamber (4°C) with primary anti-insulin antibody (Insulin Antibody #4590 4590S, Cell Signaling), anti-oligomer antibody (anti prefibrillar oligomer-like material ...
-
bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Pre-cleared lysates were incubated over night at 4°C with end-over-end rotation with 2 µg of anti-Notch1 (D1E11, Cell Signaling) or anti-Cbl-b (G1 ...
-
bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated in PBS-T containing 3% BSA (PBS-T-BSA) for 30 min and then incubated overnight at 4 °C with rabbit polyclonal anti-RPS6 antibody (2211, Cell Signaling), rabbit polyclonal anti-MRPS27 antibody (17280-AP ...
-
bioRxiv - Biochemistry 2019Quote: ... for 30 min at room temperature and incubated overnight at 4°C with Phospho-Histone H2A.X (Ser139) Antibody (Cell Signaling, 2577) in 1% BSA ...
-
DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360 ...
-
bioRxiv - Genetics 2020Quote: ... Primary antibody incubations were performed overnight at 4°C using the following antibodies: rabbit anti-GAPDH 14C10 (0.1 μg/mL, Cell Signaling 2118S), mouse anti-rhodopsin antibody clone 1D4 (1 μg/mL ...
-
bioRxiv - Immunology 2019Quote: Samples were washed twice in ice cold HBS and then lysed in 1000ml lysis buffer for 30 minutes at 4°C with end over end mixing (Cell Signaling Lysis Buffer 20mM Tris-HCL pH7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... 0.1% [vol/vol] Tween) before probing overnight at 4°C with the following antibodies: 4E-BP1 (Cell signaling technologies [CST] #9644), β-actin (CST #5125 or CST #4970) ...
-
bioRxiv - Neuroscience 2021Quote: ... Drosophila brains were fixed in 4% PFA for 10 min and prepared for staining according to the manufacturer’s protocol (Cell Signaling Technology). Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner ...
-
bioRxiv - Developmental Biology 2020Quote: ... and antibody (1.2 ug of anti-H3K4me1: Diagenode, C15410194, Lot A1862D or 4 uL anti-H3K4me2: Cell Signaling #9725, Lot 9) was added per 0.6 mL-tube ...
-
bioRxiv - Biochemistry 2022Quote: ... 400 μl of clean sample was then incubated overnight at 4 °C on gentle shaking with 20 μl of either anti-MYC-agarose beads (3400, Cell Signaling) or anti-HA-agarose beads (3956 ...
-
bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
-
bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3x with PBST and incubated overnight at 4°C under agitation with rabbit anti-phosphorylated TRKB (Cell Signaling, either #4168 against pY816 or #4619 against pY515 ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... 60 µg of chromatin was incubated overnight on a rotating wheel at 4°C with the following antibodies: normal rabbit IgG (2729S, Cell Signaling), RNA polymerase II antibody (NBP2-32080 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246; SAFB, Bethyl A300-812A; SUZ12, Cell Signaling #3737) or 3μL (7.5μg ...
-
bioRxiv - Molecular Biology 2023Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 10μL antibody (anti-SAFB, Bethyl 812-300A; EZH2, Cell Signaling #5246) or 10μg IgG (Invitrogen #02-6502) ...
-
bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
-
bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
-
bioRxiv - Developmental Biology 2021Quote: ... The following antibodies are from commercial sources: rabbit anti-cleaved Death caspase-1 (Dcp-1) (1:250, Cell Signaling); chicken anti-GFP (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... Sample was incubated with anti-Pu.1 antibody (microglia marker 1:50, Pu.1-AlexaFluor 647, 9G7 Cell Signaling) in permeabilization buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then sequentially stained for Iba-1 (1:500, 19741 Wako) and NLRP3 (1:50, D4D8T Cell Signaling) primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit ...
-
bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
-
bioRxiv - Cell Biology 2020Quote: ... A polyclonal antibody against phosphorylated Tyr416 of Src and a polyclonal antibody against mouse caspase-3 were from Cell Signaling Technology (Beverly, MA, USA). α-Thrombin was purchased from Enzyme Research Laboratories (South Bend ...
-
bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
-
bioRxiv - Neuroscience 2019Quote: ... Total and phosphorylated glycogen synthase kinase 3 (GSK3-β) were detected using rabbit antibodies anti-GSK3-β (9315) and anti-pGSK3α/β-Ser21/9 (9331) (Cell Signaling). Chemiluminescence signals were analyzed using Image Lab software 6.0 (Bio-Rad).
-
bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis, France) and rabbit polyclonal anti-cleaved caspase 3 antibody was provided by Cell Signaling Technology (Ozyme, Saint Cyr l’ecole, France). All Alexa-conjugated secondary antibodies were purchased from Thermo Fisher Scientific.
-
bioRxiv - Cancer Biology 2022Quote: ... acetyl-histone 3 (Lys27, #8173, D5E4), histone3 (#4499, D1H2), EP300 (# 86377, D8Z4E), and CREBBP (# 7389, D6C5) were purchased from Cell Signaling Technologies (Danvers, MA). An anti-acetyl-SMC3 antibody was a kind gift from Forma Therapeutics ...
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... The expression of arrestin-3 and GPCRs was determined by western blot using anti-arrestin F431 [32] anti-HA (#3724, Cell signaling Technology, Danvers, MA) antibodies.
-
bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
-
bioRxiv - Cell Biology 2022Quote: ... SRF (5147, 1:750), MRTF-A (14760S, 1:1000) and cofilin (3312, 1:1000) antibodies were from Cell Signaling Technology, Ipo9 (PAB0154 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubation with primary antibodies (collagen IV: Bio-Rad, #134001, 1:200; caveolin-1: Cell Signaling Technology, #3267, 1:400) was performed at 4 °C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... Actin (1:5000 dilution; ab179467; RRID:AB_2737344) or Vinculin (1:2000; Santa Cruz sc-73614; RRID:AB_1131294) (1:2000; Cell Signaling 13901S; RRID:AB_2728768), diluted in 5% BSA ...
-
bioRxiv - Developmental Biology 2020Quote: ... and rabbit anti-cleaved DCP-1 (9578S 1:1000; Cell Signaling Technologies) to detect plasmatocytes and apoptotic cells ...
-
bioRxiv - Cell Biology 2019Quote: ... Rabbit α-MCL-1 (4572, 1:1000) was obtained from Cell Signaling. Goat α-mouse IgG HRP (1:5000 ...
-
bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Egr-1 (15F7)(Cell Signaling Technology, Cat# 4153; 1:1000) and anti-GAPDH (D16H11 ...