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Citations for Cell Signaling Technology :
1701 - 1750 of 2256 citations for Rat Butyrophilin Like Protein 2 BTNL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
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bioRxiv - Physiology 2021Quote: ... were subjected to western blot analysis with antibodies against c-Jun NH(2)-terminal kinase (JNK) and phospho-JNK (Cell Signaling). Immunocomplexes were detected using the Licor Odyssey secondary detection system.
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bioRxiv - Cancer Biology 2021Quote: ... the degree of staining of the tissue specimens was observed under the Zeiss Apotome.2 microscope (20X objective, 0.8 NA) after DAB staining (Cell Signaling Technology) and hematoxylin (IHC World ...
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bioRxiv - Systems Biology 2020Quote: ... from femur by incubating the samples with mouse anti-Runx-2 (an osteoblast marker) antibody (1:1000 dilution in PBS containing 0.5% BSA, Cell Signaling Technology) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit antibodies against phospho (S473)-AKT (p-AKT) and phospho (Thr202/Tyr204) p44/42 ERK1/2 (p-ERK) (4060, 9101; Cell Signaling) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% BSA for one hour at RT and incubated overnight at 4°C with anti-flotillin-2 (C42A3) (1:500, #3436, Cell Signaling), anti-calnexin (1:500 ...
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bioRxiv - Plant Biology 2022Quote: ... Phosphorylated MAPK proteins were detected by immunoblot analysis with antiphospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) rabbit mAb (Cell Signaling Technology, USA). The blotted membrane was stained with Coomassie Brilliant blue (CBB ...
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bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Molecular Biology 2023Quote: ... Double resistant cell lines with dual integration of 4EBP1/2 shRNAs were validated by western blot (Cell Signaling Technology #9452, #2845) for efficient stable knockdown of the targeted proteins.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immunolabeling was realized using the rabbit monoclonal primary antibody anti-phospho-ERK1/2 Thr202/Tyr204 (#4370, Cell Signaling Technologies, 1:2,000), or anti-total ERK1/2 (#05-1152 ...
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... anti-phospho-Akt (Thr308) (C31E5E) and anti-phospho-p44/42 MAPK (Erk 1/2) (Thr202/Tyr204) (E10) antibodies (Cell Signaling Technology). Each experiment was repeated 4 times ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4°C on a rocker with primary antibodies (Supplemental Table 2) according to the manufacturer’s instructions and then developed with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 hour at room temperature on an orbital shaker ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, Cell Signaling Technology, Danvers, MA, USA,; RRID: AB_2315112), rabbit polyclonal anti-human SOD1 (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to all native and/or phosphorylated proteins (see Supplementary Table S1 for details) except for WT1 were purchased from Cell Signaling Technology (Boston, MA). The C-terminal anti-WT1 antibody (C-19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared by incubation with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling, Cat. No 9863), for 1 hour in 4oC ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Microtubule-associated proteins 1A/1B light chain 3B (LC3B-I/-II) at 1:1000 dilution (14, 16 kDa, Cell Signaling Technology, Danvers, MA); anti-monocarboxylate transporter 1 (MCT1 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Cancer Biology 2024Quote: Total protein was extracted from spheroids and organoids using lysis buffer supplemented with a protease/phosphatase inhibitor cocktail (both from Cell Signaling Technology, MA, USA). Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were visualized utilizing SignalStain DAB Substrate Kit (Cell Signaling Technology# 8059) and imaged using Zeiss AxioImagerA2 attached with AxioCam MRc camera.