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Citations for Cell Signaling Technology :
1701 - 1750 of 2687 citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator. Rabbit anti-GFP (TorryPines, cat. # TP401,) or normal rabbit IgG (Cell signaling, cat. # 2729) chemically cross-linked to protein G agarose beads were used as controls ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 3% BSA in PBS for 1 hour and cells were stained with VEGFR2 (Cell Signaling; C#2479; 1:200) and EEA1 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in 5% BSA with 1% Tween 20 for 1 hour then probed overnight at 4°C with the following primary antibodies at a 1:1,000 dilution: Phospho-STAT1 (Cell Signaling Technologies, 9167S, monoclonal), SOCS1 (Cell Signaling Technologies ...
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Specific patterns of neural activity in the hippocampus after massed or distributed spatial trainingbioRxiv - Neuroscience 2023Quote: ... After 1 h of incubation in PBST containing 1% BSA and 1% NGS sections were incubated overnight in anti-phospho-c-Fos rabbit monoclonal antibody (5348S; Cell signaling Technology, USA) diluted 1:8000 in PBST– BSA–NGS ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Microbiology 2023Quote: ... expression of hACE and cACE2 was validated via immunofluorescence and immunoblots through detection of the c-terminal myc tag with a 1:500 dilution of antibody Myc-Tag-9B11 Mouse mAb (Cell Signaling Technology, 2276).
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
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bioRxiv - Physiology 2024Quote: ... for one hour at room temperature before being incubated overnight at 4°C with the following primary antibodies: anti-phospho-p38 (1:1,000, #9211, Cell Signaling Technology, Billerica, MA); anti-p38 (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C with the primary antibody diluted in blocking buffer or SignalStain® Antibody Diluent (cat. 8112, Cell Signaling Technology). Slides were incubated with biotinylated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μg of digested chromatin samples was treated with antibodies against rabbit IgG (1:100; Cell Signaling Technology, 2729) or NKX2.1 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked with 5% milk powder in TBST for 1 h and probed with SETD2 (Cell signaling, 80290) 1:1000 overnight at 4°C with agitation ...
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bioRxiv - Systems Biology 2020Quote: ... then incubated with anti-vimentin antibody (clone D21H3, Cell Signaling Technologies, (1:500 dilution in 5% milk/TBS-T) and with anti-GAPDH antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked with 5% powdered milk in buffer and probed with pan-SynGAP (1:1,000, #5539, Cell Signaling) or SynGAP-α2 (abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then incubated with 1× TBST containing 5% milk and either anti-RPS6KB1 (Cell Signaling Technology, 2940S) or anti-DNAJC3 (Cell Signaling Technology ...
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bioRxiv - Pathology 2022Quote: ... Membranes were stained with Ponceau-S and blocked for 30 minutes with 5% nonfat dry milk (Cell signaling, 9999) or 5% BSA in Blotto buffer (0.15M NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... The following primary antibodies were used: Phospho-Smad1/5 (Ser463/465) (41D10) (1:300, Rabbit, Cell Signaling Technology #9516S), P-Histone H3 (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) (Cell Signaling Technology, 4668S, 1:2500 in 5% BSA); Rabbit polyclonal antibodies to p38 MAPK (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the MCF10A cell line by chronic stimulation with 5 ng/ml TGFβ1 (Cell Signaling Technology) for eight days22 ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with anti-NTMT1 (1:1000 dilution in 5% BSA in TBST buffer, Cell signaling #13432) at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... Each blot was blocked with 10 mL 5% (w/v) nonfat dry milk (Cell Signaling Technology, Danvers, MA, USA) in TBST buffer (25 mM Tris base ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked with 5% BSA and incubated with rabbit mTOR (1:1000, Cell Signaling Technologies, USA), rabbit p-mTOR (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% milk and probed using either 1:1000 antiV5 antibody (Cell Signaling Technology, 13202) or 1:10000 GAPDH as a loading control (Proteintech 60004-1) ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were first incubated with blocking and permeabilization buffer (1X PBS / 5% normal goat serum (Cell Signaling, cat #5425) / 0.3% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with 5% skimmed milk in Tris-Buffered Saline with Tween 20 (TBST; Cell Signaling Technology) for 1 h ...
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bioRxiv - Genomics 2023Quote: ... 1ml (5*106 cells) was aliquoted into each of 3 tubes with antibody (1.25 µg anti-HA, Cell Signaling #3724S ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blotted overnight with primary antibodies in TBS-T containing 5% BSA (Cell signaling antibodies; Stat1 (9172), phospho-Stat1-Tyr701-(7649) ...
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bioRxiv - Cell Biology 2023Quote: Cullin-RING E3 ubiquitin ligases were inhibited by addition of 5 μM MLN4924 (Cell Signaling Technology, catalog no.: 85923) and the 26S proteasome was blocked with 10 μM MG132 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked with 5% nonfat dried milk and then incubated with primary antibodies from Cell Signaling Technology that recognize ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% BSA TBST and incubated in the primary antbodies anti-INSR (Cell Signaling Technology, #3025), anti-TdTomato (SICGEN ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
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bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1β (30 ng/ml, Cell Signaling Technology, 8900SF) and TNF-α (30 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well Meso Scale Discovery assay plates (MSD, L15XA-6) were coated with 30 μl of pS65-Ub capturing antibody (Cell Signaling Technology, 62802) with a final concentration of 1 µg/ml in 200 mM sodium carbonate coating buffer (pH 9.7 ...
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bioRxiv - Cancer Biology 2023Quote: ... OV90 and CAOV3 cells were cultured in a glass bottom 96 well plate as described and stained with Cleaved caspase 3 (CC3) antibody (Cell signaling technology: 9661T) at 1:100 dilution after 12 hours incubation with T cells for CAOV3 and 48 hours incubation for OV90 cells ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cells were washed in 1x PBS and lysed on the plate by the addition of complete RIPA buffer with protease (Cell Signaling Technology #5871S) and phosphatase (Cell Signaling Technologies #5870S ...
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bioRxiv - Neuroscience 2024Quote: ... the 96-well MSD plates (Meso Scale Diagnostics, L15XA) were coated with 1 µg/mL p-S65-Ub capturing antibody (Cell Signaling Technology, 62802), blocked with 1% BSA in TBST ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
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bioRxiv - Genetics 2019Quote: ... as described previously.21 The following antibodies were used for western blotting: anti-S6 ribosomal protein (Cell Signaling Technology), anti-phospho-S6 ribosomal protein (Ser240/244 ...
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bioRxiv - Immunology 2019Quote: ... The following primary antibodies were used to probe protein levels by immunoblotting: anti-GAPDH (Cell Signaling Technology, clone D16H11), anti-β-Actin (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2020Quote: ... protein from transfected 293T cells was analyzed by western blotting with an anti-GFP antibody (clone D5.1, Cell Signaling). This corresponded with an increase in protein size from 27 to 32 kDa by western blotting (Fig S2C) ...
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bioRxiv - Immunology 2019Quote: ... 40 μg of sheared chromatin/sample was incubated with protein G magnetic beads with α-RelB (10544, Cell Signaling) or α-NF-κB2 antibodies (37359 Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: Fibroblasts and podocytes were washed twice with PBS and proteins were extracted with the lysis buffer (Cell Signaling Technology) which protease and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto nitrocellulose and incubated with primary antibody: LC3 (rabbit polyclonal, Cell Signaling, cat # 12741S, 1:1000), SQSTM1/p62 (guinea pig polyclonal ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to PVDF transfer membranes and incubated with primary antibodies against pAKT serine 470 (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...