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Citations for Cell Signaling Technology :
1651 - 1695 of 1695 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cas9 expression was validated by flow cytometry using intracellular staining of cas9 protein with anti-cas9 monoclonal antibody (7A9-3A3, Cell Signaling, cat #48796). Cas9 activity was assessed by transducing the cas9-BV2 cells with pXPR_047 ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Genetics 2022Quote: ... We quantified changes in protein levels relative to control using Image Lab software after normalization to β-actin or GAPDH (Cell Signaling Technologies), as indicated ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130; #610822 Becton Dickinson, Cytochrome C; #11940T Cell Signaling Technology, ANXA5, ItgaX; #14-011485 eBioscience) or HRP-labeled primary antibodies (TSG101 ...
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bioRxiv - Cell Biology 2023Quote: ... or the GFP antibody (HuaBio, ET1604-26; used in Figs. 5 B C D and 6 A) or GAPDH antibody (Cell Signaling Technology, #5174; used in Fig. S3 A) or p-MLC2 antibody (Cell Signaling Technology ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to all native and/or phosphorylated proteins (see Supplementary Table S1 for details) except for WT1 were purchased from Cell Signaling Technology (Boston, MA). The C-terminal anti-WT1 antibody (C-19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared by incubation with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling, Cat. No 9863), for 1 hour in 4oC ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Microtubule-associated proteins 1A/1B light chain 3B (LC3B-I/-II) at 1:1000 dilution (14, 16 kDa, Cell Signaling Technology, Danvers, MA); anti-monocarboxylate transporter 1 (MCT1 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Cancer Biology 2024Quote: Total protein was extracted from spheroids and organoids using lysis buffer supplemented with a protease/phosphatase inhibitor cocktail (both from Cell Signaling Technology, MA, USA). Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were then incubated at 28°C and the samples were collected at the indicated intervals for determination of GST and GST-WL1 protein abundance by western blots using the anti-GST (#2625, Cell Signaling Technology, 1:1000 dilution) and alkaline phosphatase-conjugated anti-rabbit (# 31340 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used to detect proteins involved in glycolysis (Hk1 clone C35C4, Glut1 clone D3J3A and HIF-1a D1S7W) were from Cell Signaling (all at 1:500) were used to intracellular staining ...
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bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
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bioRxiv - Neuroscience 2023Quote: ... which is consistent with other validation western blots published by vendors for this protein as well as manuscripts listed above (Cell Signaling Technologies, Novus, Rockland, Proteintech). The anti-SARS-CoV-2 spike protein antibody (GTX632604 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were transferred onto nitrocellulose membranes for immunoblotting and then probed with antibodies for phospho-AMPKα (Thr172) and β-Actin (Cell Signaling Technology, Danvers, MA, USA). The membrane was probed with appropriate secondary antibodies and imaged on an Odyssey Imaging System (LICOR ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... Optical densities were measured by dividing the intensity of the bands by the intensity of the housekeeping protein β-actin (Cell Signaling Technology, MA, USA, 1:1000). Data from control samples were assigned a relative value of 100%.
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bioRxiv - Zoology 2022Quote: ... The sections were then incubated with anti-phosphorylated-p44/42 mitogen-activated protein kinase (MAPK; extracellular regulated kinase, ERK1/2) rabbit monoclonal antibody (#4370, Cell Signaling Technology, Danvers, MA, USA; 1:500), which was used in zebrafish OE36 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were placed overnight at 4°C in a solution containing 2% donkey serum and primary antibodies for post-synaptic density protein 95 (PSD95; rabbit host, 1:100, Cell Signaling Technology, Danvers, MA, product #2507, lot 2), vesicular glutamate transporter 1 (VGLUT1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C in PBS containing 2% donkey serum and primary antibodies for post-synaptic density protein 95 (PSD95; rabbit host, 1:100, Cell Signaling Technology, Danvers, MA, product #2507, lot 2), and vesicular glutamate transporter 1 (VGLUT1 ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005; GRK6: Cell signaling technology #5878, Actin: Sigma-Aldrich A5441). For experiments using the MOP phospho-specific antibodies see below ...