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Citations for Cell Signaling Technology :
1651 - 1700 of 10000+ citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with methanol/acetone (50/50) and then stained intracellularly by incubating cells with rabbit antibody against p-(Y694) STAT-5 (D47E7) XP (Cell Signaling), mouse antibody against BCL-2 (100 ...
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bioRxiv - Cell Biology 2019Quote: ... 100,000 cells were plated in each well the same day of the assay and allowed to attach for 5 hours in growth media in the presence of 5μM ROCK inhibitor (13624, Cell Signaling Technology). Subsequently growth media was replaced with Seahorse base media (102353-100 ...
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bioRxiv - Genetics 2021Quote: ... containing 5% (w/v) skimmed dried milk powder and immunoblotted using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody (#2851 Cell Signaling) (dilution 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with either 5% skimmed milk or 5% bovine-serum albumin (BSA) and incubated with dilution of respective antibodies as per manufacturer’s instructions (Cell Signaling technologies). The membrane was washed ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cancer Biology 2022Quote: ... De-identified FFPE tumor specimens were cut into 5 μm sections and IHC performed as described above to stain for human EZH2 (5246, Cell Signaling) and NKp46 (AF1850 ...
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bioRxiv - Cell Biology 2022Quote: ... fresh frozen OCT-embedded embryo lung sections (5 μm thick) were stained with anti-E-Cadherin (Cell Signaling, Cat No 3195), anti-Snail (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Immunology 2021Quote: ... Cells were treated with 5% goat serum for 30 min and incubated with antibodies against TRPC3 (Alomone Labs) or NFkB-p65 (Cell Signaling) for 1h ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and the grey matter was Dounce homogenized in 5 volumes per weight (v/w) buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was then incubated with 5 μg of Anti-FLAG Antibody (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling) and 50 μL of protein G beads at 4 ºC overnight ...
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bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in 1X PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology).
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked for 1 h in 5% non-fat milk in 1X TBST at room temperature and incubated with primary antibodies against KEAP1 (#8047, Cell Signaling), NQO1 (#11451-1-AP ...
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bioRxiv - Physiology 2024Quote: ... C2C12’s were replated and allowed 24 hours to recover after which the labeled C2C12 myoblasts were induced for apoptosis using 5 µM Staurosporin (cat. no.: 9953S, Cell Signaling) for 18h ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then blocked and permeabilized for 1 hour at room temperature while rocking with 1mL of blocking buffer (0.3% Triton X 100 and 5% normal goat serum (Cell Signaling Technology) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were blocked in TBST buffer (Tris-buffered saline and 0.15% Tween-20) containing 5% nonfat dry milk (#9999, Cell Signaling Technology). The blots were probed overnight with primary antibodies diluted in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized with 100% cold methanol and stained with anti-phosphorylated (p)SMAD1/5/9 (Cell Signaling Technology, Danvers MA) overnight at a 1:50 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated overnight at 4°C with the primary antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370S, Cell Signaling) at 1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-cleared lysates were incubated over night at 4°C with end-over-end rotation with 2 µg of anti-Notch1 (D1E11, Cell Signaling) or anti-Cbl-b (G1 ...
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bioRxiv - Cancer Biology 2019Quote: ... β-Tubulin (9F3) Rabbit mAb (Cell Signaling Technology Cat# 2128, RRID:AB_823664, Lot# 7). For immunoblotting ...
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bioRxiv - Immunology 2023Quote: ... rabbit anti-caspase-7 clone D2Q3L (Cell Signaling Technology Cat#12827T, RRID:AB_2687912), mouse anti-caspase-8 clone 1C12 (Cell Signaling Technology Cat#9746S ...
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bioRxiv - Cell Biology 2020Quote: ... WB dilution 1:1,000) and P-MLC2 (T18/S19, #3674S IF dilution 1:100, WB dilution 1:500) were from Cell Signaling (Danvers, MA, USA). Rabbit monoclonal antibody directed against α4-actinin was from Life Technologies (#42-1400 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight with primary antibodies (1:500 pSTAT1, 7649 Cell Signaling; 1:500 pSTAT3, 9145 Cell Signaling; 1:500 pSTAT5, 9359S Cell Signaling; 1:500 STAT1 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500; rabbit anti-cleaved caspase-3, Cell Signaling, 1:500) overnight in 3 % BSA in PBS at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100; Mouse Tubulin 1:1000; Cell Signaling DM1A). Primary incubation was followed with HRP-conjugated secondary antibodies (Rabbit-HRP 1:10000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000, Cell Signaling; GCN5L1 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000, #2231), AKT (Ser473, 1:1000, #9271), ERK (Thr202/Tyr204, 1:2000, #4370) and STAT3 (Tyr705) (1:1000, #9131, Cell Signaling, Massachusetts, USA) and total EGFR (1:1000 ...
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bioRxiv - Physiology 2023Quote: Antibodies directed against SIK3 (#39477, 1:1000), Myc (#2278 and #2276, 1:1000) and acetylated lysine (#9441, 1:1000) were obtained from Cell Signaling Technology (CST). Antibodies against UCP1 (ab23841 ...
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bioRxiv - Developmental Biology 2023Quote: ... MoBU-1, mouse, 1:250, ThermoFisher #B35128; secondary antibody: anti-mouse IgG Alexa Fluor® 555 Conjugate, 1:500, goat, Cell Signaling #4409). First ...
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bioRxiv - Cell Biology 2023Quote: ... non-phospho (Active) β-Catenin (D13A1, 1:1000 WB), GAPDH (14C10, 1:10000 WB) and YAP (D8H1X, 1:200 IF) were from Cell Signaling Technologies. β-catenin antibody (14 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated with primary antibodies for drebrin (1:1000, Enzo, Cat#ADI-NBA-110) and GLUA1 (AMPA receptor 1, GLUA1, 1:400, Cell Signaling Cat#13185) in blocking buffer overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody used was early endosome antigen 1(EEA1 – 1:200; Cell Signaling) and the secondary antibody was Alexa647 AffiniPure donkey anti-rabbit IgG (1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with antibody (Drosha, Cell Signaling, 1:50 or Safb1, Abcam 1:50 resp.) on a rotating wheel overnight at 4°C (where 2.5% of each sample had been saved as input) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-Myc 9B11 at 1:2000-1:3000 (Cell Signaling Technology, Danvers, MA), mouse anti acetylated α-tubulin 6-11B-1 Alexa488 conjugated 1:50 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-E-cadherin (clone 24E10, 3195S, Cell Signaling; IB 1:1000; IHC 1:400), anti-N-cadherin (14215S ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were anti-Cofilin-1 mAB (clone D3F9, 1:100, Cell Signaling, 5175), anti-GRP75 mAB (clone N52A/42 ...
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bioRxiv - Neuroscience 2020Quote: ... Flotillin-1 rabbit polyclonal antibody (D2V7J, Cell Signaling, Cat# 18634, 1:1K for WB); β-actin mouse monoclonal antibody (AC-74 ...
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bioRxiv - Immunology 2020Quote: ... anti-total-NF-κB p65 (1:1□000 dilution, 6956T, Cell Signaling Technology, USA), anti-phospho-TRAF-associated NF-κB activator (TANK)-binding kinase 1 (TBK1 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... was used for experiment 1 while a monoclonal rabbit antibody (1:5000, Cell signaling) was used for experiment 4 ...
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bioRxiv - Developmental Biology 2019Quote: ... and rabbit anti-Dcp-1 (1:200, Asp216, Cell Signaling Technology, Danvers, MA, USA). Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2019Quote: ... H2AX pS139 (γH2AX) (Santa Cruz sc-101696, 1:500; Cell Signaling #2577 1:1000); p53 pS15 (Cell Signaling #9284 ...
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bioRxiv - Pathology 2020Quote: ... Superoxide dismutase (SOD)-1-specific mouse monoclonal antibody (1:1000 dilution, 71G8, Cell Signaling), Heat shock protein (HSP)-60-specific rabbit monoclonal antibody (1:1000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissues were incubated with α-PD-1 antibody (Cell Signaling Technology, 1:250) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-phospho-S166 mouse RIPK1 (Cell Signaling Technology 31122; 1:2000/1:200), mouse anti-GAPDH (Millipore MAB374 ...