Labshake search
Citations for Cell Signaling Technology :
1651 - 1700 of 1809 citations for 7 chloro 4 oxo 4H chromene 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Rabbit antibodies for anti-myosin-light-chain-2 (Cat #3672) and anti-phospho-myosin-light-chain-2 (raised against phospho-Thr18/Ser19; Cat #3674) were purchased from Cell Signaling and used at 1:500 (Western blot) ...
-
bioRxiv - Cell Biology 2022Quote: ... then seeded in DMEM/F12 and 1% serum plus antibiotic-antimycotic solution 48 hrs post-transfection cells were blocked and treated with Ab against α5β1 (Novus, 2-52680) and αv (Cell Signaling 4711) at 10ug/ml for 30 minutes prior to cell surface stripping (0.2M acetic acid ...
-
bioRxiv - Immunology 2021Quote: ... followed by washing and 2 hour incubation with goat-anti-rabbit-HRP antibody at 1:5000 (all from Cell Signaling Technology). Membranes were washed again and detected with SuperSignal West Pico Chemiluminescent substrate (Thermofisher) ...
-
bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
-
bioRxiv - Plant Biology 2021Quote: ... the membrane was incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, #9101). Subsequently ...
-
bioRxiv - Developmental Biology 2020Quote: ... blocked and incubated with an antibody recognizing Myosin Light Chain 2 phosphorylated at serine19 (1:200, rabbit; Cell signaling technology, #3671), or with the anti-tyrosinated tubulin antibody YL1/2 (1:50 ...
-
bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
-
bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were washed with TBST prior to incubation for 2 hrs at room temperature in the appropriate secondary antibody (anti-rabbit or anti-mouse; Cell Signaling) at 1:2000 dilutions ...
-
bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
-
bioRxiv - Microbiology 2019Quote: ... Where stated, cells were treated with 2 μM ruxolitinib (INCB018424, Stratech, S1378-SEL) or 50 nM bortezomib (Cell Signaling Technology #2204) or 1000 units of IFNβ (pbl Assay Science ...
-
bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Isl1/2 (Developmental Studies Hybridoma Bank, 1:100) and rabbit anti-β3-tubulin (Cell Signaling Technology, 5568S 1:200).
-
bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
-
bioRxiv - Microbiology 2020Quote: ... 250 μL of lysate were incubated with 2 μL of mouse Myc-Tag antibody clone 9B11 (Cell Signaling Technology, Cat. 2276S) for 4 h with rotation at 4°C ...
-
bioRxiv - Bioengineering 2020Quote: ... 1 x 108 induced cells were stained with 1 µM of directly AF488-labeled SARS-CoV-2 RBD and 0.5 µM anti-HA AF647 (Cell Signaling Technology) antibody ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
-
bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...
-
bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
-
bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
-
bioRxiv - Physiology 2021Quote: ... were subjected to western blot analysis with antibodies against c-Jun NH(2)-terminal kinase (JNK) and phospho-JNK (Cell Signaling). Immunocomplexes were detected using the Licor Odyssey secondary detection system.
-
bioRxiv - Cancer Biology 2021Quote: ... the degree of staining of the tissue specimens was observed under the Zeiss Apotome.2 microscope (20X objective, 0.8 NA) after DAB staining (Cell Signaling Technology) and hematoxylin (IHC World ...
-
bioRxiv - Systems Biology 2020Quote: ... from femur by incubating the samples with mouse anti-Runx-2 (an osteoblast marker) antibody (1:1000 dilution in PBS containing 0.5% BSA, Cell Signaling Technology) at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit antibodies against phospho (S473)-AKT (p-AKT) and phospho (Thr202/Tyr204) p44/42 ERK1/2 (p-ERK) (4060, 9101; Cell Signaling) for 30 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
-
bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
-
bioRxiv - Plant Biology 2022Quote: ... Phosphorylated MAPK proteins were detected by immunoblot analysis with antiphospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) rabbit mAb (Cell Signaling Technology, USA). The blotted membrane was stained with Coomassie Brilliant blue (CBB ...
-
bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
-
bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
-
bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
-
bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, Cell Signaling Technology, Danvers, MA, USA,; RRID: AB_2315112), rabbit polyclonal anti-human SOD1 (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Double resistant cell lines with dual integration of 4EBP1/2 shRNAs were validated by western blot (Cell Signaling Technology #9452, #2845) for efficient stable knockdown of the targeted proteins.
-
bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immunolabeling was realized using the rabbit monoclonal primary antibody anti-phospho-ERK1/2 Thr202/Tyr204 (#4370, Cell Signaling Technologies, 1:2,000), or anti-total ERK1/2 (#05-1152 ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... anti-phospho-Akt (Thr308) (C31E5E) and anti-phospho-p44/42 MAPK (Erk 1/2) (Thr202/Tyr204) (E10) antibodies (Cell Signaling Technology). Each experiment was repeated 4 times ...
-
bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4°C on a rocker with primary antibodies (Supplemental Table 2) according to the manufacturer’s instructions and then developed with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 hour at room temperature on an orbital shaker ...
-
bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
-
bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
-
bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
-
bioRxiv - Cancer Biology 2019Quote: ... blocked in 8% goat serum and incubated overnight at 4°C with anti-phospho Axl (R&D, AF2228, diluted 1:200) CK11 (Cell signaling, CST 4545, diluted 1:200), followed by fluorescently labelled secondary antibodies goat anti mouse 488 (Abcam ab98637) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
-
bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
-
bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...