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Citations for Cell Signaling Technology :
1651 - 1700 of 10000+ citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... Crude cell extract was lysed with an insulin syringe and pre-cleared by incubation with 3 μg Normal Rabbit IgG (Cell Signaling Technology) and 10uL Pierce™ Protein A/G Magnetic Beads (Thermo Scientific 88803 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rabbit, pan, 40D4), phospho-AKT [rabbit, Ser473, (D9E)XP] and GAPDH (glyceraldehyde-3-phosphate dehydrogenasemouse, D4C6R) were purchased from Cell Signaling (USA). Antibodies against human PCNA (proliferating cell nuclear antigen ...
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bioRxiv - Cancer Biology 2024Quote: ... Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology for immunoblot or fluorescence imaging analysis ...
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bioRxiv - Bioengineering 2024Quote: ... The antibodies against phospho-STAT3 (Tyr705, 9145), cleaved caspase-3 (Asp175, 9579) and PCNA (2586) were purchased from Cell Signaling Technology (USA). The antibodies against myeloperoxidase (ab208670 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Developmental Biology 2023Quote: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: Immunoblot experiments were performed as previously described (17, 18) using antibodies against cleaved caspase-3 (Cell Signaling Technology, Cat#9664, RRID: AB_2070042), procaspase-3 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then labeled overnight at 4℃ with primary antibodies for neurofilament-heavy (RT-97, Developmental Studies Hybridoma Bank, University of Iowa) or cleaved caspase-3 (Cell Signaling 9664S). After three rounds of washing ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 45 min with 3 μg/ml Myc-Tag (9B11) Mouse mAb AlexaFluor 488 conjugate antibody (Cell Signaling Technology) and Phalloidin-Alexa 647 (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Cells were treated and prepared as described above for synchronization and flow cytometry with the addition of cleaved caspase-3 antibody (Cell Signaling Technology) conjugated to Alexa Fluor 647 added together with pH3 antibody ...
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bioRxiv - Cell Biology 2024Quote: ... The required primary antibody (JOSD1 [Abcam, Cambridge, United Kingdom], SOCS1 [Abclonal, Woburn, Massachusetts, United States of America], Cleaved Caspase 3 [Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit DA1E monoclonal antibody IgG isotype control (cat. # 3900) and cleaved caspase-3 rabbit monoclonal antibody (cat.# 9664) were all from Cell Signaling Technology. Anti-Iba1/AIF1 mouse monoclonal antibody (used for immunofluorescence in xenografts ...
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bioRxiv - Neuroscience 2022Quote: ... DCTN1 (mouse 1:200 Santa Cruz 135890, rabbit 1:1000 or 1:50 Cell signaling 69399) and β III-tubulin (1:6000 Biolegend 802001) ...
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bioRxiv - Cell Biology 2024Quote: ... HALTag Rabbit mAb (1:500; #3724), GAPDH Rabbit mAb (1:2000; #5174), β-actin (1:2000; #3700), H3 Rabbit mAb (1:2000, #4499) (Cell Signaling Technology), S-Nitrocysteine mAb (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight with primary antibodies (1:500 pSTAT1, 7649 Cell Signaling; 1:500 pSTAT3, 9145 Cell Signaling; 1:500 pSTAT5, 9359S Cell Signaling; 1:500 STAT1, 9172 Cell Signaling; 1:500 STAT3 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated overnight at 4°C with antibodies for the designated antigens INTEGRIN α5 (1:1000, #4705), INTEGRIN β3 (1:1000, #4702), β-ACTIN(1:1000, #8457), β-TUBULIN (1:1000, #2146) purchased from Cell Signaling Technology, GAPDH (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by primary antibody incubation (SOX2, 1:500, Bio-techne, AF2018; SOX9, 1:500, Merck, AB5535; SOX9, 1:500, R&D Systems, AF3075; TP63, 1:400, Cell Signaling Technology, 13109 ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma)/PBSTX and incubated for 48 hours at 4°C in rabbit anti-Cx43 primary antibody (Cat. No. 3512; Cell Signaling) diluted 1:100 in 1% BSA/PBSTX ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were immunoblotted overnight at 4 °C on a shaker with antibodies against hif1a (Catalog # 14179, Cell Signaling Technology), cleaved caspase 3 (Asp-175 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then incubated at 4◻C overnight with primary antibodies against pSTAT1 (pY701; clone D4A7; Cell Signaling 7649S), total STAT1 (Clone D1K9Y ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells where incubated overnight at 4°C with rabbit anti-phospho-STAT1 (Tyr701) primary antibody (Cell Signaling Technology, 9167S) diluted 1:100 in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were incubated overnight at 4 °C with the rabbit monoclonal anti-K17 antibody (D12E5, Cell signaling, Danvers, MA). After incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... They were incubated overnight at 4°C with anti-mouse monoclonal rabbit antibody against Ki-67 (#12202, Cell Signaling Technology) at dilutions of 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal protein concentration were incubated overnight in 4 °C with an anti-Src antibody (Cell Signaling Technology, #2108). Complexes were pulled out using protein-A sepharose beads (GE-Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were incubated over-night at 4 °C with desired primary antibody (cat. no. #9662, #55506; Cell Signaling Tech-nologies ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed 3 times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL EZH2 antibody (anti-EZH2, Cell Signaling #5246) or 20μg IgG (anti-IgG ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were fixed with 4% paraformaldehyde for 5 minutes and blocked in 5% goat normal serum (Cell Signaling Technologies, 5425) for an hour before antibody detection using antibodies listed in Supplementary Tables S5-S6 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then probed overnight at 4°C with the following primary antibodies: rabbit anti-LSD1 (C69G12, Cell Signaling Technology), rabbit anti-MAO-A (10539-1-AP ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were collected and analyzed on 4-20% SDS-PAGE with anti-GFP antibodies (Cell Signaling Technologies D5.1, #2956). Anti-α-tubulin antibodies (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated in 4% milk supplemented with either anti-mouse IgG HRP-conjugated secondary antibody (Cell Signaling Technologies, 7076P2) (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies (synaptophysin Cat#MAB329, Merck Millipore; GluA2 Cat#13607, Cell Signaling; Psd95 Cat#MAB1598 ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C with the following primary antibodies: rabbit D4E1 monoclonal anti-RyR1 (Cell Signaling Technology, catalog N°8153 ...
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bioRxiv - Immunology 2024Quote: ... and then incubated overnight at 4°C with antibodies directed at RelA (p65) protein (2.1μg/mL; Cell Signaling Technology, 8242) and its respective isotype control antibody (2.1 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit anti-NKX2-1 (1:2000; Cell Signaling Technology 12373), rabbit anti-PROX1 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300 ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies against Calpain 1 (1:1000, #2556, Cell signaling Technology), Calpain 4 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Fos (1:5000, Calbiochem; 1:2000, Cell Signaling), guinea pig anti-Fluorogold (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... and rabbit anti- Cav-1 (1:1000, Cell Signaling, USA) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... and phospho-ERK 1/2 (Cell Signaling, RRID:AB_331646; 1:1000).) ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-cleaved Dcp-1 (Cell Signaling 9578, 1:200), mouse anti-γH2Av (DSHB UNC93-5.2.1 ...
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... rb-anti-Dcp-1 (1:50; Cell Signaling Technology #9578), Developmental Studies Hybridoma Bank ...
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bioRxiv - Pathology 2021Quote: ... Histone H3 (Cell Signaling, 9715, 1:1000 in 1% milk). Membranes were washed in Blotto buffer and incubated with the appropriate HRP-conjugated secondary antibody for 75 minutes at room temperature ...