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Citations for Cell Signaling Technology :
1551 - 1600 of 1650 citations for Rat Angiopoietin 2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
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bioRxiv - Neuroscience 2024Quote: ChIP-seq analysis from P35-42 mouse cortex was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003) following manufacturer’s instructions with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Fixation and staining were performed using a β-galactosidase staining kit per the manufacturer’s instructions at pH 6.0 (Cell Signaling). Cells were counterstained with DAPI to enable quantification.
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bioRxiv - Developmental Biology 2020Quote: ... and incubated with annexin V and propidium iodide (PI) as per the manufacturer’s instructions (Early Apoptosis Detection Kit, Cell Signaling Technology). Cells were analyzed by flow cytometry using a BD FACSCanto cell analyzer (BD Biosciences) ...
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bioRxiv - Physiology 2022Quote: The acetyl peptide enrichment was performed on 3 mg of digested peptides using PTMscan Acetyl-Lysine Motif kit (Cell Signaling #13416). The peptides were mixed with 100 µL of 10x IP buffer (500 mM MOPS ...
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bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal activity staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
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bioRxiv - Systems Biology 2019Quote: ... and monomethyl arginine peptides were immunoprecipitated by addition of 40 uL of PTMScan Symmetric Di-Methyl Arginine Motif Kit (13563, Cell Signaling), PTMScan Asymmetric Di-Methyl Arginine Motif Kit (13474 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
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bioRxiv - Cell Biology 2021Quote: Chromatin immunoprecipitation assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit - Magnetic Beads (Cell Signaling Technology, Inc., Danvers, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Senescence-Associated β-galactosidase activity was assessed using a SA-β-galactosidase staining kit according to the manufacturer’s instructions (Cell Signaling). Positive cells were counted manually out of more than 100 cells total.
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the HRP/ peroxidase enzyme was performed with SignalStain® DAB Substrate Kit from Cell Signaling (cat. no. 8059; www.cellsignal.com), per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: CUT&RUN experiments were conducted using 200,000 PS or PSP cells using the CUT&RUN assay kit (Cell Signaling Technology, 86652). Briefly ...
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bioRxiv - Genomics 2022Quote: ... Cleaved Caspase1 and cleaved Gasdermin D antibodies were purchased as a part of Pyroptosis Antibody Sampler Kit (#43811T, Cell Signaling Technology). After overnight primary incubation ...
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bioRxiv - Cancer Biology 2019Quote: MEFs were prepared from E13.5 embryos and senescence of MEFs was determined by using the Senescence β-Galactosidase Staining Kit (Cell Signaling) according to the manufacturer’s protocol57 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... All ChIP was performed using the SimpleChIP Enzymatic Chromatin IP kit with magnetic beads according to the manufacturer’s instructions (Cell Signaling Technologies). DNA levels were quantified by qPCR amplification using Power SYBR Green Mastermix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: GTP-bound GTPase pull-down assays were performed using the Active Rac1/CDC42 Detection Kit (Cell Signaling Technology, Cat. #8815, 8819). After transfection or verteporfin treatment ...
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bioRxiv - Neuroscience 2020Quote: ... or WT N2aC plated in chamber slides using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology, Danvers, MA). As a positive control for apoptosis and necrosis ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Developmental Biology 2023Quote: ChIP-qPCR and ChIP-seq samples were prepared following the instructions of the SampleChIPR Enzymatic Chromatin IP Kit (Cell Signaling, #9005). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Senescence-associated β galactosidase assay was performed according to the manufacturer’s protocol and as described above (Cell Signaling Technology, Kit #9860). 5,000 cells were plated on each well of a 96-well dish ...
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bioRxiv - Bioengineering 2023Quote: A glutathione assay was developed for in-chip measurements by adapting the Cellular Glutathione Detection Assay Kit (Cell Signaling Technology #13859). The monochlorobimane reagent was prepared by reconstitution in DMSO per manufacturer’s directions ...
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bioRxiv - Genomics 2023Quote: CUT&RUN experiments were conducted using the CUT&RUN (Cleavage Under Targets & Release Using Nuclease) assay kit (Cell Signaling Technology, #86652) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: CUT&RUN (Cleavage Under Targets & Release Using Nuclease) libraries were prepared with the established CUT&RUN Assay Kit (Cell Signaling, #86652S). The following antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
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bioRxiv - Molecular Biology 2023Quote: The chromatin immunoprecipitation (ChIP) assay was performed with SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell signaling technology, #9005) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: K-ε-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-Є-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described [62] ...
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bioRxiv - Developmental Biology 2021Quote: ChIP assays were performed in WT and Arp5-KO RD cells using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... quantified with Quantitative Colorimetric Peptide Assay (Thermo Fischer Scientific) and dried on Speed-Vacuum before enrichment with Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling). After Sep Pak desalting ...
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bioRxiv - Physiology 2020Quote: ChIP assays of frozen liver tissues of humanized mice were performed using a Simple ChIP Enzymatic Chromatin IP kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... Immuno-precipitation was performed for two replicates for each genotype (about 1 g tissue/IP) by SimpleChIP Plus Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: The chromatin immunoprecipitation (ChIP) assays was performed using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technologies, Inc., MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ChIP-seq experiments were performed with 108 HDFs per replicate using a SimpleChip Plus sonication chromatin IP kit (Cell Signaling Technology, 56383) according to the manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti TRA-1-81 (1:200) and mouse anti-SSEA4 (1:200) (all from Cell Signaling Technology, StemLight Pluripotency Antibody Kit) to assess for pluripotency ...
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bioRxiv - Molecular Biology 2022Quote: Lysates from parental and resistant cells were used to screen activation of receptor tyrosine kinases using the PathScan® RTK Signaling Antibody Array Kit (7982, Cell Signaling) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for acetylation with anti-acetyl antibody conjugated to agarose beads (Acetyl-Lysine Motif Kit; Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: Fragmental DNA for ChIP-seq was prepared using SimpleChIP®Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technologies, Cat#9005) per manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... was used as secondary for 30 minutes and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling, Danvers, MA).
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed three times with ice-cold PBS and subcellular fractionation was carried out using a Cell Fractionation kit following manufacturer instructions (Cell Signaling, #9038).
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bioRxiv - Cell Biology 2021Quote: Acylated (either acetylated or malonylated) peptides were enriched by immunoaffinity purification using the PTMScan Acetyl-Lysine or PTMScan Malonyl-Lysine Kits (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Proliferating and Senescent mouse fibroblasts were washed twice with 1X PBS and fixed and stained for B-Gal activity using the Senescence B-Galactosidase Staining Kit per the manufacturer’s instructions (Cell Signaling Technology 9860S). The following day cells were washed twice with 1X PBS and stained with Hoechst (Thermo Fisher Scientific 62249) ...
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bioRxiv - Cell Biology 2021Quote: The staining of SA-β galactosidase in cells at pH-6 was performed using a senescence β-Galactosidase staining kit according to the instructions of the manufacturer (Cell signaling). Measurement of the absorbance at 420 nm from SA-β galactosidase activity detectable at pH 6.0 was performed as described (Lee et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... and acetylation were sequentially enriched and purified using PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology #8803), PTMScan® Ubiquitin Remnant Motif (K-ε-GG ...
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bioRxiv - Physiology 2022Quote: The chromatin immunoprecipitation assay was performed as previously reported using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, Cat No. 9003)47 ...