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Citations for Cell Signaling Technology :
1551 - 1600 of 2267 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The blots were incubated overnight at 4 degrees Celcius in a 1:2000 dilution of anti-AUF1 antibody (Cell Signaling D6O4F). The blots were washed (3 × 10 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were diluted with lysis buffer without inhibitors and incubated overnight at 4°C with anti-PCNA antibody (1:500, #2586, Cell signaling) under mild agitation ...
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bioRxiv - Immunology 2021Quote: ... Fixed and permeablized cells were then incubated at 4 ̊C with anti-phospho-S6 (Ser235/236) APC (clone D57.2.2E; Cell Signaling Technology #14733). Samples were acquired on a BD LSRII flow cytometer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney, and brain were homogenized at 4°C (Precellys Cryolys Evolution, Bertin Instruments) in lysis buffer (RIPA buffer, 1X, Cell Signaling). Tissue total protein concentration was measured by BCA assay (Pierce BCA) ...
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bioRxiv - Neuroscience 2022Quote: ... in primary antibodies for 48 hours at 4°C [Rabbit anti-P-4E-BP (1:600; Cell Signaling Technology, Danvers, MA), goat anti-MAP-1A (1:360 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked in 5% non-fat dry milk in TBS-T and incubated over night at 4°C with anti-GFP (1:1,000; Cell Signaling Technologies) or anti-Actin (1:4,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% milk in PBST for 1 hour at room temperature and incubated overnight at 4°C with agitation in primary antibody at the following dilutions: rabbit anti-His (1:1,000, Cell Signaling, 2365), rabbit anti-GST (1:1,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated overnight at 4°C with the following antibodies: rabbit anti-phospho-Akt (Ser473) (1:1000; clone D9E, Cell Signaling), rabbit anti-Akt (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and subsequently incubated overnight at 4 °C with respective primary antibodies: pan-AKT antibody (40D4) (1:2,000; 2920S; Cell Signaling Technology), phospho-AKT (Ser473 ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 h of paraquat treatment cells were fixed with 4% PFA and stained for cleaved caspase 3 (Cell Signaling, 9661).
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bioRxiv - Neuroscience 2022Quote: ... the slices were then incubated for 48 hr at 4 °C with diluted primary antibody for c-fos (9F6) (anti-rabbit, 1:1000; Cell Signaling). Third ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies were diluted in blocking solution overnight at 4°C: phos-NFkB p65 (Ser536) (93H1) Rabbit mAb (1:1000) (Cell Signaling), phos-Stat3 (Tyr705 ...
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bioRxiv - Developmental Biology 2022Quote: ... Discs were incubated with primary antibodies overnight at 4°C: rabbit anti-Cleaved Drosophila cDcp-1 (1:200, Cell Signaling, #9578S), rabbit anti-fkh (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated in PBS-T containing 3% BSA (PBS-T-BSA) for 30 min and then incubated overnight at 4 °C with rabbit polyclonal anti-RPS6 antibody (2211, Cell Signaling), rabbit polyclonal anti-MRPS27 antibody (17280-AP ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated overnight at 4°C with the following primary antibody: rabbit anti-IKKα (Cell Signaling 2682; 1:1,000), rabbit anti-phospho-IKKα/β (Ser180/Ser181 ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4) for 2.5 hours and 100 μm thick sagittal sections were collected and incubated in DRAQ5 (1:1000, Cell Signaling Technology) on ice for an hour ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C using primary antibodies (rabbit anti-p S6 (S235/236) 1:1000 (Cell Signaling #4858), rabbit anti-p-S6 (S240/244 ...
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bioRxiv - Genetics 2020Quote: ... Primary antibody incubations were performed overnight at 4°C using the following antibodies: rabbit anti-GAPDH 14C10 (0.1 μg/mL, Cell Signaling 2118S), mouse anti-rhodopsin antibody clone 1D4 (1 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% [vol/vol] Tween) before probing overnight at 4°C with the following antibodies: 4E-BP1 (Cell signaling technologies [CST] #9644), β-actin (CST #5125 or CST #4970) ...
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bioRxiv - Cancer Biology 2021Quote: ... Thermo Fischer Scientific MA5-11690) overnight at 4°C and then incubated with goat anti-rabbit HRP linked IgG (1:10000, Cell signaling). Membranes were visualized using a ChemiDoc MP (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Drosophila brains were fixed in 4% PFA for 10 min and prepared for staining according to the manufacturer’s protocol (Cell Signaling Technology). Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner ...
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bioRxiv - Developmental Biology 2020Quote: ... and antibody (1.2 ug of anti-H3K4me1: Diagenode, C15410194, Lot A1862D or 4 uL anti-H3K4me2: Cell Signaling #9725, Lot 9) was added per 0.6 mL-tube ...
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bioRxiv - Neuroscience 2022Quote: ... 10% normal donkey serum in 1xPBS) and then incubated overnight at 4°C with a rabbit polyclonal antiserum against cFos (1:1000, Millipore and Cell Signaling) followed by a Cy3-conjugated donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... 400 μl of clean sample was then incubated overnight at 4 °C on gentle shaking with 20 μl of either anti-MYC-agarose beads (3400, Cell Signaling) or anti-HA-agarose beads (3956 ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3x with PBST and incubated overnight at 4°C under agitation with rabbit anti-phosphorylated TRKB (Cell Signaling, either #4168 against pY816 or #4619 against pY515 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Cell Biology 2024Quote: ... solution one hour at room temperature and subsequently incubated overnight 4°C with primary antibodies: anti-S6 (1:10,000; 5G10 monoclonal rabbit; Cell Signaling 2217), anti-Phospho-S6 (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... we used a rabbit monoclonal anti-HA antibody (1:500 in 0.3 % Triton in PBS; 48 h incubation at 4 °C; #3724, Cell Signaling Technology), followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor pieces were fixed in 4% PFA prior to paraffin embedding and then stained with GR (1:1000, Cell Signaling, D6H2L) on a bond III automated stainer (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246; SAFB, Bethyl A300-812A; SUZ12, Cell Signaling #3737) or 3μL (7.5μg ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 10μL antibody (anti-SAFB, Bethyl 812-300A; EZH2, Cell Signaling #5246) or 10μg IgG (Invitrogen #02-6502) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and incubated overnight at 4°C with the corresponding primary antibody diluted in blocking solution: Anti-Caspase 3 (rabbit, 1:100, Cell Signaling). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation in 5% FBS in PBS for 16 h at 4°C with primary antibodies (1:1,000) to the following: LAMP1 (Cell Signaling, 9091), p62 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-c-Fos (1:3000, RRID:AB_2247211, Cell Signaling Technology, USA), mouse anti-NeuN (1:800 ...
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with primary antibodies at 4°C overnight: anti-HIF-1α (1:1000, Cat #36169, Cell Signaling Technology, USA) or anti-β-Actin (1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C with the primary c-Fos antibody (1:1000, Rabbit, Cell Signaling #2250). After 3 rinses with PBS for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by incubation with primary antibodies at 4 °C overnight (the primary antibodies were rabbit anti-GAPDH antibody, 1:2000, Cell Signaling, Cat#5174 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blocked in 5% dry milk in TBS-T for 30 min and incubated overnight at 4°C with primary antibodies against: rabbit V5 at 1:1,000 (Cell Signaling Technology), mouse vinculin at 1:1,000 (Millipore Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated overnight at 4 °C with the following primary antibodies diluted in Superblock/0.1% Tween 20: p-JNK (1:1000; Cell Signaling Technologies), total-JNK(1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: Aortae were perfusion-fixed in situ with 4% paraformaldehyde (overnight) prior to staining with primary beta-catenin antibody (Cell signaling: 9562) and fluorophore-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were next incubated overnight at 4 °C with Shh (C9C5) rabbit antibody (1:500, Cell Signaling Technologies, Cat. No. 2207) in PBS-BSA-T ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4 % PFA at passage 1 and stained for the endothelial markers CD31 (Cell Signaling 3528S, 1:800), VE-cadherin (2500S ...