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Citations for Cell Signaling Technology :
1501 - 1534 of 1534 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... cells were stained with senescence-associated (SA)-β-galactosidase (gal) according to the manufacturer’s instructions for Senescence β-Galactosidase Staining Kit (Cell signaling, 9860). Cells were counterstained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: SUM159 and MDA-MB-231 cells were stained for SA-β-gal detection using a Senescence β-galactosidase Staining Kit (#9860, Cell Signaling). Staining was performed by following the manufacturer’s instructions and incubating the stained samples for 48hrs at 37°C without CO2 ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Senescence-associated beta-galactosidase in HSVSMC lysates was quantified using the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) (Cell signaling Technology). Measurement was done on cell lysates with the same total protein concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-rat secondary antibody was used for detection of primary antibodies and visualized by SignalStain DAB Substrate kit (Cell Signaling Technology, Danvers, MA). All stained tissues were blindly evaluated by pathologists ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen sections were hydrated with PBS for 10min at RT and stained using Senescence β-Galactosidase staining kit according to manufacturer’s instructions (Cell signaling technology, 9860). The sections were incubated at 37℃ in a dry incubator (no CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cellular lysates from FLSs were harvested and a ChIP assay was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with an appropriate HRP-conjugated secondary antibody (Cell signalling technologies, cat. n. 7074) and LumiGLO® ECL kit (Cell Signaling Technology) was employed following manufacturer’s instruction before to exposure to X-ray film (CytivaTM) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were dried by lyophilization and resuspended in IAP buffer from PTMScan HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). K-GG containing heavy reference peptides were pooled and diluted into the IAP buffer resuspension fat a final concentration of 1 fmol per peptide ...
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bioRxiv - Genetics 2021Quote: ... and myotube (4 days differentiation in low serum differentiation medium) and ChIP was performed using the Simple ChIP Enzymatic Chromatin IP Kit (Cat# 9003; Cell Signaling Technology; Danvers, MA) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2021Quote: Approximately 1.2 million microglia were exposed to the indicated treatments followed by cell preparation according to the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003). Samples were cross-linked for 10 min at room temperature with 1% formaldehyde solution ...
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bioRxiv - Cell Biology 2020Quote: ... Specificity of C12FDG detecting senescent cells was determined in comparison with results obtained with the senescence β-galactosidase staining kit (Cell Signaling Technology #9860) on IMR90 cells that were treated with 100 ng/mL doxorubicin (Sigma ...
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bioRxiv - Genetics 2019Quote: ChIP followed by allele-specific quantitative PCR (ChIP AS-qPCR) [38] were performed in U2OS cell line by using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalog# 9003) (Cell Signaling Technology, USA) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the Foxp3 transcription factor staining kit (eBioscience, 00-5523-00) prior intracellular staining with the indicated antibodies: H3K9ac (Cell Signaling, 9649, 1:3000), H3K27ac (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for malonylation with anti-malonyl antibody conjugated to agarose beads from the Malonyl-Lysine Motif Kit (Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The following steps were performed according to the manual of the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). In brief ...
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bioRxiv - Cell Biology 2023Quote: SA-β-gal staining was performed using a SA-β-gal staining kit according to the manufacturer’s protocol (Cell Signaling Technology, Danvers, MA, USA). In short ...
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bioRxiv - Cancer Biology 2023Quote: IMR90 cells seeded on the plate were fixed and stained for SA-β-gal activity with the senescence β-Galactosidase Staining Kit according to the protocol (Cell Signaling, #9860S).
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... that were treated with PBS or Dox or transduced with adenovirus harboring shRNA scramble (shCtrl) or shRNA Atg7 (shAtg7) were stained with the SA-β-gal staining kit (Cell Signaling Technologies, #9860), according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest was split into 8 aliquots dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784) as described in the manual and peptides were eluted twice with 200µL of 50% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2024Quote: RAS activity was assessed by affinity pull-down of endogenous levels of GTP-bound Active-Ras as described by Active Ras detection kit (Cell Signaling Technology, cat# 8821).
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bioRxiv - Cancer Biology 2019Quote: ... cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). For chromatin preparation from tissue ...
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bioRxiv - Immunology 2024Quote: ... and kept at 4 °C for up to 10 days until chromatin fragmentation using SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting DNA fragment samples were generated into libraries using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell Signaling Technology, 56795) and SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-GM130 (BD transduction Laboratories, ref:610822, 1:1000, rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref ...
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bioRxiv - Cell Biology 2023Quote: ... Senescence-associated β-Galactosidase (SA β-gal) activity was measured in the hepatocytes using Senescence β-Galactosidase Staining Kit (Cell Signaling Technology-9860, Danvers, MA, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then resuspended in 100 μL of cell lysis buffer from the Cyclic AMP XP® Assay Kit (Catalog# 4339S, Cell Signaling Technology, Danvers, MA, USA) on ice for 10 minutes ...