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Citations for Cell Signaling Technology :
1451 - 1500 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were horse anti-mouse HRP or goat anti-rabbit HRP which were both diluted 1:1,000 in block (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Cell Biology 2021Quote: ... Washed membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies as indicated (1:2000, Cell Signaling Technology, Beverly, MA) in 10 ml of blocking buffer with gentle agitation for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody binding was directed against eGFP using a solution containing a 1/4000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, USA). For all VLPs ...
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bioRxiv - Immunology 2020Quote: ... IL-1β mouse mAb (3A6) and monoclonal rabbit anti-caspase-1 (catalog no. 2225) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Immunology 2022Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Biochemistry 2023Quote: ... were used at a concentration of 1:1000 and visualized using HRP-conjugated secondary antibodies (anti-rabbit [Cell Signaling, 7074S] or anti-mouse [Cell Signaling, 7076S]) at a concentration of 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat # 69295) at (1:1000), anti-Rab27B (E4V30, Cat # 17572), and α-Tubulin Mouse mAb (DM1A, Cat#3873) all purchased from Cell Signaling Technology. The anti-Rab27B (Cat # 13412-1-AP ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were washed three times with PBST and incubated with either 1 μg/ml of rabbit monoclonal anti-mouse TREM2 (Cell Signaling Technology, 76765) for full length Trem2 ELISA (ELISA 1) ...
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bioRxiv - Biochemistry 2023Quote: ... The bound primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Catalog# 7076S) by chemiluminescence (VWR) ...
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bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
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bioRxiv - Microbiology 2023Quote: ... expression of hACE and cACE2 was validated via immunofluorescence and immunoblots through detection of the c-terminal myc tag with a 1:500 dilution of antibody Myc-Tag-9B11 Mouse mAb (Cell Signaling Technology, 2276).
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBS-Tween three times for 10 min at RT and incubated with the secondary antibody anti-mouse-HRP (1:5000) (Cell Signaling, Danvers, MA) for one hour at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Four-micrometer thick sections were serially cut from formalin-fixed paraffin-embedded mouse tumor samples to perform single IHC studies using antibodies recognizing: Phospho-KNL1 (1:100, rabbit monoclonal antibody, Cell Signaling, Cat# 40758), and Cleaved Caspase-3 (1:400 ...
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bioRxiv - Molecular Biology 2024Quote: ... which were then incubated at room temperature for 1 hour with appropriate secondary antibody dilutions (anti-mouse or anti-rabbit, Cell Signaling, Danvers, MA). Following activation in chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... membranes that had been washed three times 20 minutes in TBS with 0.1% Tween 20 at room temperature were incubated with HRP-conjugated goat anti-mouse antibody (Cell Signaling Technology; 1:5000). The chemiluminescent signal was developed using the Clarity Western ECL substrate (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... the cells were washed thrice with 1x PBS and incubated with Alexa Fluor-594 conjugated secondary antibody (1:200 dilution, Anti-mouse IgG, Cell Signaling Technology, #8890) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Protein A agarose beads (Cell Signaling Technologies 9863S) were washed and incubated in NET150 buffer (20 mM Tris HCl ph ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal protein S6 (#2217 CST) from Cell Signaling. Mouse monoclonal anti-beta Actin AC-15 HRP (#AB49900 ...
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bioRxiv - Molecular Biology 2021Quote: ... or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G agarose beads (Cell Signaling) were washed in IP buffer and blocked with bovine serum albumin (BSA) ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A HRP conjugate (Cell Signaling Technology) diluted at 1:5000 in blocking buffer for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G agarose beads (Cell Signaling Technology) were then added and incubated for 2 hr at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... S6 Ribosomal protein (Cell Signaling Technologies, Cat. # 2217S), p4EBP1 (S65 ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS–polyacrylamide gel electrophoresis and the proteins were detected with the following antibodies: LOXL2 (Cell Signaling; 99680S), BRD4 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg/mL of recombinant human IgG1 Fc or Siglec-11 Fc chimera protein was precomplexed with 0.5 µg/mL of Protein A -HRP (Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... followed by incubation with 1:2,500 dilution of primary antibodies for CD68 (rat anti-mouse, ABD SeroTec) and cleaved Casp3 (rabbit anti-mouse, Cell Signaling) in 0.5% w/v BSA in 1X PBS for 24 h at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-linked anti-mouse IgG (Cell Signaling, #7076).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti phospho IκBα (9246, Cell Signaling Technology), rabbit anti IκBα (9242 ...
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bioRxiv - Immunology 2021Quote: ... and rabbit anti-mouse Ly6G (E6Z1T, Cell Signaling) antibodies diluted in PBS 0,1%Tween20 followed by α-rat 647 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... and anti-mouse-HRP secondary (Cell Signaling 7076). GFP was normalized to total protein bands per lane (visualized by a stain-free scan on a Bio-rad gel doc) ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... and mouse anti-PCNA (Cell Signaling Technology, #2586) was diluted in 1% BSA with 0.3% Triton™ X-100 in PBS and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and caspase-3 (Mouse, 966S1; Cell Signaling Technology). Secondary antibodies were similar to the ones used with cells ...
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bioRxiv - Physiology 2020Quote: ... and anti-mouse Alexa 488 (Cell Signaling, 4408) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies: Anti-Mouse pSTAT1 (Y701) (Cell Signaling #9167s), Anti-Mouse STAT1 (Cell Signaling #9172S) ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse CD8 (D4W2Z; Cell Signaling Technology), rat anti-mouse CD45R (clone B220 ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse mAb anti-human RAS (Cell Signaling Technology). Compounds SHY-855 and SHY-867 were synthesized at Enamine LLC (Kiev ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-β-actin (Cell Signaling Technologies, #3700), rabbit anti-β-actin (Cell Signaling Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-Myc-Tag (9B11) (2276, Cell Signaling), rabbit anti-HA-Tag (C29F4 ...