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Citations for Cell Signaling Technology :
101 - 150 of 170 citations for Sodium hypochlorite solution cp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight in rabbit anti-c-Fos primary antibody (1:200 in blocking solution, Cell Signaling Technologies, Ab #2250). Following overnight incubation in primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and incubated overnight at 4°C with the corresponding primary antibody diluted in blocking solution: Anti-Caspase 3 (rabbit, 1:100, Cell Signaling). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min and after destaining the membranes were incubated at 4°C over night with primary antibodies in the respective concentration using 1xTBS containing 0.1% Tween 20 including LC3 A/B (Cat. No. #4108S, dilution 1:1000 in blocking solution, Cell Signaling Technology), p62 (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was rapidly weighed and homogenized with a pestle homogenizer in the following solution: 1x Cell Lysis Buffer (Cell Signaling, 9803S), 0.5% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242 ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated overnight in the saturation solution containing the primary antibody (rabbit anti c-Fos (1/1500, Cell Signaling), sheep anti-BrdU (1/2500 ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed 3X in PBS and blocked for 1 h at RT with 5% BSA solution containing 5% normal goat serum (Cell Signaling) and 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... They were rinsed with 1x PBS again and incubated with 50 μL of blocking solution (1x PBS, 0.5% goat serum) with 1:300 anti-rabbit mTOR (Cell Signaling Technology, #2983S) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of each substrate solution was added to 1 mL of PBS or RIPA lysis buffer (Cell Signaling Technology, Danvers, MA) for NLuc experiments ...
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bioRxiv - Bioengineering 2022Quote: ... We cultured tricultures for 7 days and subsequently stained them with CD31 and phalloidin (7 μL per 1000 μL solution) or cytokeratin 18 (CK18; 1:250, Cell Signaling 24E10) using the protocol described above ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Pathology 2023Quote: ... They were then incubated first with blocking solution for 1 hr and then with a rabbit polyclonal anti-RelA antibody (8242; Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was stopped by adding 1× stop buffer (digitonin solution, RNase A, and 50 pg S. cerevisiae spike-in DNA (Cell Signaling Technology) for normalization ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution using the following concentrations: 1:1000 anti β-actin (13E5) rabbit primary antibody (#4970 from Cell Signaling), 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara) ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution (1:1000 anti β-actin (13E5) rabbit primary antibody #4970 from Cell Signaling) (1:1000 anti GAPDH ...
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bioRxiv - Physiology 2023Quote: ... Membranes were washed in TBST and incubated with HRP - conjugated anti-rabbit IgG at 1:2000 dilution in blocking solution (#7074, Cell Signaling Technology). Bands were revealed with ECL Prime reagent (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were rinsed with pre-warmed 1x PBS and fixed with 1x fixative solution provided by senescence beta-galactosidase staining kit (9860, Cell Signaling Technology) for 15 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody solution contained a 1/1000 dilution of a monoclonal rabbit anti-HA antibody (C29F4, Cell Signaling Technology, Danvers, USA) in Tris-buffered saline (TBS ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in ice-cold 70% ethanol for at least 30 minutes and were then labeled with propidium iodide (PI)/RNase staining solution (#4087, Cell Signaling Technology) and further incubated for 15 minutes at 37°C ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... cells were incubated in the blocking solution with the HRP linked anti-mouse or anti-rabbit secondary antibody (respectively #7076 or #7074; Cell Signaling Technology) at 1:10,000 dilution (1 hr at RT in the dark) ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT# ...
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bioRxiv - Genomics 2024Quote: ... Separate PCLS were fixed for 30 minutes in the fixative solution from the β-galactosidase staining kit (Cell Signaling Technology Cat#9860) and then washed in PBS.
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... each gel was rinsed briefly in deionized water and then stored for >12 hours in 1X tris-buffered saline solution with Tween® (TBST, prepared from Cell Signaling Technology 9997S 10X stock and MilliQ ultrapure water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody binding was directed against eGFP using a solution containing a 1/4000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, USA). For all VLPs ...
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bioRxiv - Genetics 2020Quote: ... 500 μl of PI (Propidium Iodide) /RNase solution was added and incubated with the cells for 20 min at room temperature (Cell Signaling Technology, 4087). The cell suspension was then applied onto FACS tubes with cell strainer cap (Corning ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Molecular Biology 2024Quote: ... brains were stained with primary antibody (NC82) diluted in antibody solution (970µL of PBST, 30µL NGS, 1µL rabbit anti-GFP (Cell Signaling Technology, Cat # 2956S, 1:1000), 50 µL mouse anti-DLG (1:20) ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were saturated with the blocking solution followed by a 1hr at 37°C incubation with the primary antibodies: Bcl-xL (Cell Signaling #2764, 1/1000) and IP3R C-terminal (abcam #ab190239 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with blocking solution for 90min then with a combination of two primary antibodies: rabbit anti-Fos (1:2000; Cell Signaling Technology, RRID #2250) and chicken anti-mCherry (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Physiology 2020Quote: ... The blots were washed and immunoreactive bands were detected by incubation with anti-rabbit HRP conjugated antibody (dilution 1:2000 in blocking solution; Cell signaling, Cat no. 7074S) for 1h at room temperature ...