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Citations for Cell Signaling Technology :
101 - 150 of 730 citations for Recombinant Cyan Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Neuroscience 2024Quote: ... the top five recombinant p-S65-Ub antibodies or the reference antibody (Cell Signaling Technology, 62802, E2J6T) were used as capturing agents ...
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Molecular Biology 2022Quote: Synchronized young adult animals expressing N-terminal HA-tagged PMK-1 were used for immunoprecipitation with anti-HA-Tag (C29F4) rabbit mAb (Cell Signaling Technology 11846) and eluted with 2x laemmli buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% milk in PBST for 1 hour at room temperature and incubated overnight at 4°C with agitation in primary antibody at the following dilutions: rabbit anti-His (1:1,000, Cell Signaling, 2365), rabbit anti-GST (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg/ml of recombinant antibodies and 0.2 μg/ml of the reference antibody (Cell Signaling Technology, 62802) were used ...
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bioRxiv - Microbiology 2022Quote: ... fluorescent-labelled Alexa Fluor 647 (AF647) goat anti-Rabbit IgG monoclonal antibody (Cat. n° 4414, Cell Signaling Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
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bioRxiv - Genomics 2020Quote: ... rabbit anti-GATA1 (NF that was produced against recombinant N-terminal zinc finger76, rabbit anti-GAPDH (14C10, Cell Signaling) and anti-β3 integrin (sc-14009 ...
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bioRxiv - Cell Biology 2020Quote: ... The fluorescent secondary antibodies were conjugated with either Alexa Fluor 594 (1:1,000, anti-rabbit, Cell Signaling Technology, 8889) or Alexa Fluor 647 (1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... by analysis of fluorescent signals of antigen-bound (Streptavidin-647) and total nanobodies (anti-HA-Alexa488, Cell Signaling Technologies) displayed on the yeast surface.
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bioRxiv - Neuroscience 2022Quote: ... Some slices were additionally incubated for 20 min with the fluorescent DNA dye DRAQ7™ (1:100, Cell Signaling #7406) at RT and washed twice for 10 min in 0.1 M PB (pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Cell Biology 2023Quote: ... we used either HRP-conjugated protein A or HRP-conjugated protein G (both from Cell Signaling) because of high background from the immunoglobulin bands when we used secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... S6 Ribosomal protein (5G10, Cell Signaling), BIM (C34C5 ...
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bioRxiv - Immunology 2019Quote: ... Protein A agarose beads (Cell signaling) were used for IgG pulldown ...
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bioRxiv - Physiology 2022Quote: ... and Biotinylated Protein ladder (Cell Signaling) were included on the gels ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysis buffer (Cell Signaling Technology) containing a protease inhibitor cocktail was added ...
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bioRxiv - Cell Biology 2023Quote: ... one for each protein (Cell Signaling). Each membrane piece was then incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with a 1:500 dilution of Alexa Fluor® 488-labeled rabbit anti-His tag antibody (Cell Signaling Technology, Danvers, MA) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
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bioRxiv - Immunology 2019Quote: ... Quantified protein (between 200-500 µg) was incubated with protein-specific or isotype control antibody (Cell Signaling Technology) in lysis buffer at 4°C overnight with head over tail rotation ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins bound to Protein-A Dynabeads were pulled down using a magnetic separation rack (Cell Signaling, no. 14654). After 5 washes in NP-40 lysis buffer ...
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bioRxiv - Immunology 2023Quote: The protein concentration was adjusted to 30 μg by adding protein lysis buffer (Cell Signaling Technology, Danvers, MA) and 4x loading buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then rinsed three times with PBS before adding 100 ng/mL DAPI and fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:500 in PBS containing 1% BSA and 0.3% Triton-X for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Separate sets of slides are stained for imaging in each fluorescent channel with an anti-PSMA rabbit primary antibody (D4S1F, Cell Signaling Technology) and either an IRDye680LT goat anti-rabbit (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: Protein A magnetic beads (Cell Signaling Technology) were pre-washed with cell lysis buffer twice ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (2217; Cell Signaling Technology), Akt1 (2938 ...
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bioRxiv - Immunology 2021Quote: ... coupled to Protein G beads (Cell Signaling) overnight with rotation at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... protein A/G agarose beads (Cell Signaling) were added and allowed to rotate for another 2h ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed x3 in PBS or TBS and incubated with fluorescent secondary antibodies and dyes (DAPI, Cell Signaling Technology; Phalloidin, ThermoFisher) in blocking solution ...
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bioRxiv - Microbiology 2022Quote: ... The fluorescent-labelled Alexa Fluor 647 (AF647) goat anti-Rabbit IgG monoclonal antibody was from Cell Signaling Technologies (MA, USA; Cat. n° 4414).
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bioRxiv - Immunology 2020Quote: ... The same amount of protein was immunoprecipitated with anti-NEMO and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2022Quote: Protein A agarose beads (Cell Signaling Technologies 9863S) were washed and incubated in NET150 buffer (20 mM Tris HCl ph ...
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bioRxiv - Developmental Biology 2022Quote: ... S6 Ribosomal Protein (1:1000, Cell Signaling #2217), LEF1 (1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal protein S6 (#2217 CST) from Cell Signaling. Mouse monoclonal anti-beta Actin AC-15 HRP (#AB49900 ...
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bioRxiv - Molecular Biology 2021Quote: ... or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G agarose beads (Cell Signaling) were washed in IP buffer and blocked with bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2019Quote: ... When blotting for total proteins (Cell Signaling Technology; EGFR #4267 ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A HRP conjugate (Cell Signaling Technology) diluted at 1:5000 in blocking buffer for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... S6 ribosomal protein (Cell Signaling 2217, 1:1000), phospho-S1859 CAD (Cell Signaling 70307 ...
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bioRxiv - Cell Biology 2021Quote: ... S6 ribosomal protein mouse mAB (2317S, Cell Signaling), phospho-p70 S6 kinase (Thr389 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G agarose beads (Cell Signaling Technology) were then added and incubated for 2 hr at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (1:1000; 54D2; Cell Signaling), BIM (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... penicillin/streptomycin and 2% Fetal bovine serum (for vehicle group) or the same media with 100ng/ml recombinant colony-stimulating factor 1 (Csf1, Cell Signaling, Boston, MA, USA). Csf1 stock was diluted in saline.