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Citations for Cell Signaling Technology :
101 - 150 of 195 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation in 5% FBS in PBS for 16 h at 4°C with primary antibodies (1:1,000) to the following: LAMP1 (Cell Signaling, 9091), p62 (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743 ...
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bioRxiv - Immunology 2024Quote: ... Krt16-/- or WT littermate mice were treated with single (at 0 h) or double (at 0h and 24h) applications of 20 μl of 0.2 mg/ml of TPA (Cell Signaling Technology), on the dorsal side of ear skin ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed 3X in PBS and blocked for 1 h at RT with 5% BSA solution containing 5% normal goat serum (Cell Signaling) and 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed with TBS-T and incubated for 1 h at room temperature with peroxidase conjugated anti-mouse or anti-rabbit IgG (1:5000 dilution, 7076 and 7074, Cell Signaling). Blots were developed using Advanced Molecular Image Imager and ChemoStar Software ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h at room temperature and then incubated overnight at +4°C with primary antibody: anti-Snai1 (Cat# 3879, Cell Signaling), anti-STIM1 (Cat# 4119 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by a 1 h incubation in the HRP-labelled secondary antibodies (horse anti-mouse IgG (Cell Signaling Technology, Cat. No: 7076), and goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... The following secondary antibodies and their respective dilutions were used: Anti-mouse IgG(H+L) DyLight 800 conjugate (Cell Signaling Technologies, #5257), 1:20000 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween-20 in PBS) for at least 1 h and then incubated in 1:100 anti-pSmad1/5/9 (Cell Signaling, #13820) in FBS-PBST at 4°C overnight ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... were obtained at 30 h pt and incubated overnight with the anti-CD2v monoclonal antibody mix or anti-HA antibody (3724; Cell Signaling Technology). Pull down products were obtained by eluting in Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% Tween 20) for 1 h at RT and then incubated with specific antibodies (eIF2α, P-eIF2α (Cat# 9722, 9721S, Cell Signaling™), HA (Cat#901501 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: ... 24 h later cells were treated with increasing concentrations (20, 40, 80 µg/ml) of α-IFITM2 (Cell Signaling, Cat#13530 S) or Remdesivir (Selleck Chemicals Cat#S8932 ...
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bioRxiv - Cancer Biology 2020Quote: ... DyLight 800 conjugate (1:15000, #5257) and anti-rabbit IgG(H+L) DyLight 680 conjugate (1:15000, #5366) from Cell Signaling Technologies. The images were captured using Odyssey CLx Imager (LICOR Biosciences).
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
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bioRxiv - Genetics 2022Quote: ... followed by 1 h incubation in horseradish peroxidase–conjugated secondary antibody (anti-rabbit or anti-mouse, #7074 and #7076, Cell Signaling Technology) diluted in PBS-T 5% milk at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Genomics 2023Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...
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bioRxiv - Cancer Biology 2023Quote: ... TritonX-100 in PBS for 1 h at room temperature and then incubated with primary antibody p-STAT-1 (Tyr701) (1:500; Cell Signaling Technology), or CD3χ (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with 5% milk in TBST for 1 h at room temperature and then probed overnight at 4°C with pStat1 (1:1000; Cell Signaling #9167S), β-actin (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were subsequently reacted with anti-C15orf48/NMES1 (NBP1-98391) antibody for 24 h or anti-LC3 (PM036; MBL) or anti-TOM20 (42406; Cell Signaling Technology) antibody for 1 h ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA, 1:1000, #5661, Cell Signaling Technology, USA ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated for 1 h at room temperature with secondary goat anti-rabbit horseradish peroxidase antibody (Cell Signaling Technology) at 1:10,000 in 5% BSA-TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1 h incubation with the HRP-conjugated secondary antibody (Cell Signaling 7076S, 1:10,000 in 2.5% milk in TBS-T). After three washes in TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Genomics 2024Quote: The CD34+ cells were treated with TAK-243 for 48 h and the cells were collected and immobilized on magnetic beads conjugated with a specific antibody against ATF4 (Cell signaling; (D4B8) Rabbit mAb #11815 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed with TBS-T before staining with secondary anti-rabbit IgG (H+L) DyLight 800 4X PEG Conjugate (Cell Signaling #5151) at 1:20,000 at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Samples were blocked in 1 % BSA in PBS for 3 h in a hydration chamber followed by primary antibody staining with pStat1 (Tyr701, Cell Signaling #9167) at 1:200 dilution overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 16 h with primary antibody (PPARα, Santa Cruz Biotechnology cat# sc-398394; histone H3, Cell Signaling Technologies cat# 14269) diluted 1:1000 in tris-buffered saline (0.5% Tween ...
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bioRxiv - Cancer Biology 2024Quote: ... Fish with tumors were euthanized after anesthesia and characterized by histopathological hematoxylin and eosin (H&E) staining and immunohistological analysis or immunofluoresence using antibodies against PCNA (Cell Signaling, D3H8P), tyrosine hydroxylase (Pel-Freez Biologicals ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated for 1h in 100 μL PBST with secondary anti-rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate, Cell Signaling). Stained cells were then washed twice with PBST and resuspended in 300 μL of PBS with 5 μg/mL DAPI to stain cells for DNA content.
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa 555-Goat anti-Mouse IgG (H+L) Secondary Antibodies were used at 1:500-1:1000 for IF (Cell Signaling Technology). DyLight 680-Goat anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated with anti-rabbit Alexa Fluor 488 and anti-mouse IgG (H+L) Fab2 Fragments Alexa Fluor 594 (Cell Signaling, 8890S), washed ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1β (30 ng/ml, Cell Signaling Technology, 8900SF) and TNF-α (30 ng/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... (TTBS) for 1 h at ambient and stained using a Phospho-Akt (Ser473) (D9E) and Histone H3 (D1H2) (all Cell Signaling Technologies, UK) diluted 1:1000 in 5% BSA in TTBS ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular phospho-stainings were performed 1 h at room temperature in the dark with antibodies against phospho-STAT5 (Tyr694; D47E7 XP Rabbit mAb #4322; Cell Signaling, 1:150), phospho-S6 ribosomal protein (Ser235/236 ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated (for 1 h) at room temperature with SignalStain® Boost Detection Reagent (HRP, cat. no. #8114; Cell Signaling Technologies, Inc.). Sections were washed three times with wash buffer and developed with freshly prepared and filtered 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were subsequently blocked with 5% milk or 5% bovine serum albumin for 1 h at room temperature and probed separately with specific primary antibodies to AKT1 (1:1,000 dilution, 75692, Cell Signaling Technology, Danvers, MA), pan-AKT (1:1,000 dilution ...