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Citations for Cell Signaling Technology :
1401 - 1450 of 10000+ citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was then washed with Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and blocked in 5% nonfat dry milk (Cell Signaling Technology, #9999) diluted with TBS-T for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in TBXT buffer containing 5% non-fat milk and horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Cell signaling tech.) and detected by using ECL Western Blotting Substrate (Pierce).
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bioRxiv - Molecular Biology 2022Quote: ... 5 minutes each at room temperature with agitation and then incubated with Anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling 7076S) in 2% milk powder in PBST and incubated at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... or 5 million cells for histone modifications was sheared with SFX250 Sonifier (Branson) and immunoprecipitated with antibodies targeting H3K27ac (#8173, Cell signaling Technology), H3K4me1 (#5326 ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were separated by electrophoresis on 10% or 12% SDS-polyacrylamide gels and transferred to PVDF membranes that were probed with anti-pSmad1/5/8 (Cell Signaling 9511S), anti-BMP4 (Santa Cruz sc12721) ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were diluted in either 5% goat serum/PBS-T or SignalStain Boost IHC Detection Reagent (8114S, Cell Signaling Technology (CST)) ...
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bioRxiv - Developmental Biology 2021Quote: ... Both Anti-ERVK-env mouse monoclonal (HERM-1811-5, Austral Biologicals, San Ramon, CA, USA) and anti-CDH1 rabbit monoclonal (Cell Signaling, 3195S) antibody were diluted 1:250 in PBS + 1% BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449}, H3K4me3 {Millipore 04-745} and H2AUb {Cell Signaling 8240S}) ...
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bioRxiv - Immunology 2020Quote: ... cells were cytospun 5’ at 500 rpm and senescence-associated beta-glalactosidase activity measured as recommended by the manufacturer (Cell Signaling Technology) on a Zeiss-Axio inverted phase-contrast microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... NCI-H522 and NCI-H1299 cells were treated with 5 μg/mL Poly I:C sodium salt (Cell Signaling Technologies Cat No. 61401) for 6 and 12 hours respectively.
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were then washed three times for 5 min with PB and mounted with a cover glass using ProLong (Cell Signaling Technology).
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bioRxiv - Systems Biology 2024Quote: ... anti-MHC Class I (clone EMR8-5, cat#: 88274) and anti-PSMB9 (clone E7J1L, cat#: 87667) were purchased from Cell Signaling Technology. Puromycin (cat# ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocyte activation to a neurotoxic phenotype was induced in cells at 4-5 DIV by incubation with TNFα (25 ng/ml; Cell Signaling Technology), IL-1α (3 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... the fixed cells were blocked in PBS containing 5% bovine serum albumin (BSA) and then incubated with mouse anti-HA antibody (6E2, Cell signaling Technology) with 1:800 dilution overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... were incubated overnight (16 hours) in 1X TBST supplemented with either 5% non-fat dry milk or bovine serum albumin (#9997, #9998, Cell Signaling Technology) at 4○C with constant rocking according to the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk and incubated with primary antibodies overnight and incubated with anti-rabbit secondary antibody from Cell Signaling Technologies for one hour after which blots were developed using Bio-Rad ChemiDoc™ Imaging Systems ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SF3B1 (1:500 dilution; D221-3, MBL Life Science) and anti-phosph-SF3B1(Thr313) (1:500 dilution; D8D8V, Cell Signaling Technology). After washing three times with PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:1000) and TRIM46 (#1 in text: Abcam Cat# ab307967, RRID:AB_3096363, 1:200-1:1000, #2 in text: Cell Signaling Technology Cat# 92574, RRID:AB_3096364, 1:200), rabbit polyclonal antibodies against βIV-spectrin (M.N ...
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bioRxiv - Cell Biology 2019Quote: ... anti-MEK1/2 rabbit antibody (no. 9122, Cell Signaling Technology, 1:1,000 dilution); anti-phospho-MEK1/2 (Ser217/221 ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-phospho-p44/42 MAPK (Erk1/2) (1:200, Cell Signaling Technologies). As for γ-H2AX or phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Developmental Biology 2020Quote: ... and p44/42 Erk1/2 at 1:1000 dilution (Cell Signaling Technology, 4696). Secondary infrared-dye antibodies were used at 1:10,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... and p44/42 Erk1/2 at 1:700 dilution (Cell Signaling Technology, 4696) for 30 minutes at 4°C followed by incubation with species-specific secondary antibodies Alexa Fluor 568 or 594 goat anti-mouse IgG at 1:200 dilution (Invitrogen ...
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bioRxiv - Physiology 2019Quote: ... anti-p44/42 MAPK (Erk1/2) Antibody (1:2,000; #9102, Cell Signaling Technology), anti-Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight with antibodies against ERK1/2 (1:2000, Cell Signaling Technology), MEK1/2 (Cell Signaling Technology) ...
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bioRxiv - Pathology 2021Quote: ... and phospho-p44/42 MAPK (ERK1/2) (Cell Signaling Technology, 4370, 1:500), anti-Catalase (R&D ...
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bioRxiv - Microbiology 2022Quote: ... rabbit-anti-Rab35 (1:1000; Cell Signaling, 9690; or Proteintech, 11329-2-AP), mouse-anti-β-actin (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... and Phospho-Myosin Light Chain 2 (Ser19) (rabbit, Cell Signaling, 1:1,000, 3671S) antibodies for 72 h at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Phospho-Myosin Light Chain 2 (Ser19) (1:50, Rabbit, Cell Signaling Technology #3671S), α-Rho1 (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-phospho-myosin light chain 2 (Thr18/Ser19) (1:500, 3674S, Cell Signaling), anti-alpha-tubulin (1:500 ...
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bioRxiv - Systems Biology 2019Quote: ... anti-Phospho-MEK1/2 (Ser217/221) (1:1000; 9154, Cell Signaling Technology, RRID:AB_2138017), anti-MEK1/2 (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: ... anti-p44/42 MAPK (Erk1/2) (1:1000; 4695, Cell Signaling Technology, RRID:AB_390779), anti-Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-phosphorylated Erk1-2 (Thr180/Tyr182) (Cell Signaling Technology, #4370; 1:100 dilution), anti-phosphorylated P38 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... ERK using p44/42 Erk1/2 antibody (1:3,000; 3A7, Cell Signaling Technologies). Phosphorylation status of FAM126A in each treatment was quantified as pFAM126A S485 signal/FLAG signal and reported relative to that of in DMSO treated FAM126A WT sample ...