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Citations for Cell Signaling Technology :
1351 - 1400 of 1665 citations for Potassium 3 4 difluorophenyl trifluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Cell Biology 2024Quote: ... solution one hour at room temperature and subsequently incubated overnight 4°C with primary antibodies: anti-S6 (1:10,000; 5G10 monoclonal rabbit; Cell Signaling 2217), anti-Phospho-S6 (1:10,000 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Neuroscience 2023Quote: ... we used a rabbit monoclonal anti-HA antibody (1:500 in 0.3 % Triton in PBS; 48 h incubation at 4 °C; #3724, Cell Signaling Technology), followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor pieces were fixed in 4% PFA prior to paraffin embedding and then stained with GR (1:1000, Cell Signaling, D6H2L) on a bond III automated stainer (Leica Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246; SAFB, Bethyl A300-812A; SUZ12, Cell Signaling #3737) or 3μL (7.5μg ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 10μL antibody (anti-SAFB, Bethyl 812-300A; EZH2, Cell Signaling #5246) or 10μg IgG (Invitrogen #02-6502) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation in 5% FBS in PBS for 16 h at 4°C with primary antibodies (1:1,000) to the following: LAMP1 (Cell Signaling, 9091), p62 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-c-Fos (1:3000, RRID:AB_2247211, Cell Signaling Technology, USA), mouse anti-NeuN (1:800 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with primary antibodies at 4°C overnight: anti-HIF-1α (1:1000, Cat #36169, Cell Signaling Technology, USA) or anti-β-Actin (1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C with the primary c-Fos antibody (1:1000, Rabbit, Cell Signaling #2250). After 3 rinses with PBS for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by incubation with primary antibodies at 4 °C overnight (the primary antibodies were rabbit anti-GAPDH antibody, 1:2000, Cell Signaling, Cat#5174 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blocked in 5% dry milk in TBS-T for 30 min and incubated overnight at 4°C with primary antibodies against: rabbit V5 at 1:1,000 (Cell Signaling Technology), mouse vinculin at 1:1,000 (Millipore Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated overnight at 4 °C with the following primary antibodies diluted in Superblock/0.1% Tween 20: p-JNK (1:1000; Cell Signaling Technologies), total-JNK(1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Revert stain was then removed and then membranes were blocked in 5% BSA for 2 hours at RT and then incubated overnight at 4°C in either anti-NLRP3 antibody (15101S, 1:1000 in 5% BSA, Cell Signaling), anti-NLRC4 antibody (12421 ...
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bioRxiv - Molecular Biology 2024Quote: Aortae were perfusion-fixed in situ with 4% paraformaldehyde (overnight) prior to staining with primary beta-catenin antibody (Cell signaling: 9562) and fluorophore-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were next incubated overnight at 4 °C with Shh (C9C5) rabbit antibody (1:500, Cell Signaling Technologies, Cat. No. 2207) in PBS-BSA-T ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4 % PFA at passage 1 and stained for the endothelial markers CD31 (Cell Signaling 3528S, 1:800), VE-cadherin (2500S ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibody incubation of the sample was carried out overnight at 4°C with the following primary antibody dilutions: rabbit anti-pSMAD1/5/9 1:200 (mAb #13820, Cell signaling, (Genikhovich et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with the different antibodies overnight at 4 °C (anti-hSYK #2710, anti-hPhospho-SYK #2712 from Cell Signaling were used according to manufacturer’s instructions) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in Tris-buffered saline with Tween-20 (0.1%), and subsequently incubated (overnight, 4 °C) with primary antibodies for p-Akt (S473) (1:1000, Rabbit mAb, Cell Signaling, 4060S), Akt (pan ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 0.1% Tween 20 (TBST) and probed with primary antibodies overnight at 4 °C using rabbit NLRP3 (1:1000, D4D8T-15101, Cell Signaling), mouse caspase 1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 to 250 µg of chromatin were incubated overnight on a rotator at 4°C with Ezh2 antibody (Cell Signaling Technology). On the next day ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated overnight at 4° C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems ...
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bioRxiv - Genomics 2024Quote: ... 0.05% Tween-20) and incubated overnight at 4°C with primary antibodies: rabbit anti-HIS tag (D3I10) mAb (12698, Cell Signaling); mouse anti-β-tubulin (clone E7 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h at room temperature and then incubated overnight at +4°C with primary antibody: anti-Snai1 (Cat# 3879, Cell Signaling), anti-STIM1 (Cat# 4119 ...
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bioRxiv - Cell Biology 2024Quote: ... rocking at 4°C (1:1000 rabbit anti-GFP and 1:500 mouse anti-EEA1, or 1:1000 mouse anti-GFP (Cell Signaling) and 1:1000 rabbit anti-GM130 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then blocked in 5% normal goat serum/0.1% saponin for 1 hr at room temperature and incubated with combinations of the indicated primary antibodies overnight at 4°C: rabbit monoclonal anti-ECAD (clone 24E10; 1:1,000; Cell Signaling Technology), mouse monoclonal anti-HLA-G (clone 4H84 ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Cell Biology 2020Quote: ... A polyclonal antibody against phosphorylated Tyr416 of Src and a polyclonal antibody against mouse caspase-3 were from Cell Signaling Technology (Beverly, MA, USA). α-Thrombin was purchased from Enzyme Research Laboratories (South Bend ...
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bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
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bioRxiv - Neuroscience 2021Quote: ... gift from Carmen Birchmeier and Thomas Müller of the Max Delbrück Center for Molecular Medicine in Berlin) and rabbit anti-cleaved Caspase-3 (1:1000, Cell Signaling Technology Cat# 9661, RRID:AB_2341188). SCs were then washed in PBS and incubated for 2 h at RT with secondary fluorescent antibodies (goat anti-rabbit-conjugated 649 ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis, France) and rabbit polyclonal anti-cleaved caspase 3 antibody was provided by Cell Signaling Technology (Ozyme, Saint Cyr l’ecole, France). All Alexa-conjugated secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... acetyl-histone 3 (Lys27, #8173, D5E4), histone3 (#4499, D1H2), EP300 (# 86377, D8Z4E), and CREBBP (# 7389, D6C5) were purchased from Cell Signaling Technologies (Danvers, MA). An anti-acetyl-SMC3 antibody was a kind gift from Forma Therapeutics ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... The expression of arrestin-3 and GPCRs was determined by western blot using anti-arrestin F431 [32] anti-HA (#3724, Cell signaling Technology, Danvers, MA) antibodies.
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Neuroscience 2024Quote: ... To assess apoptosis-associated neurodegeneration cortical brain sections were stained with active capase-3 primary antibodies (ac3, 1:100, Cell Signaling Technologies, catalog: #9662). All brain sections were also stained with a marker for neuronal nuclei (NeuN ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were washed twice with incubation buffer (0.5% BSA in PBS) and stained with an anti-cleaved caspase-3 Alexa Fluor® 488 antibody (Cell Signaling, 9603, 1:50) for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed 3 times for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibody (Cell Signaling Technology, London, UK) at a dilution of 1:20,000 in non-fat dry milk ...