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Citations for Cell Signaling Technology :
1301 - 1350 of 1514 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... tyrosine-phosphorylated STAT3 (pY705) was stained using a murine monoclonal antibody labeled with AlexaFluor 647 (Cell Signaling #4324, 1:100 dilution). Cells were analyzed on a flow cytometer (Cytoflex S ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then blocked and permeabilized for 1 hour at room temperature while rocking with 1mL of blocking buffer (0.3% Triton X 100 and 5% normal goat serum (Cell Signaling Technology) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized with 100% cold methanol and stained with anti-phosphorylated (p)SMAD1/5/9 (Cell Signaling Technology, Danvers MA) overnight at a 1:50 dilution ...
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bioRxiv - Immunology 2024Quote: ... was added for 10 minutes to break the AMPK-Sestrin2 binding followed by incubation with the anti-Sestrin2 antibody (1:100, #8487, Cell Signaling) and secondary anti-rabbit IgG-HRP conjugated antibody (1:500) ...
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bioRxiv - Genetics 2019Quote: ... Rabbit anti-human vinculin monoclonal antibody (0.1 μg/ml, Cell Signaling, Danvers, MA, USA) and Alexa Fluor 680 Dye (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 1μg/ml leupeptin) supplemented with protease and a phosphatase inhibitor cocktail (Cell Signaling #9803S). Proteins were electrophoresed on SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ precast gel (Biorad #456-1094,) ...
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bioRxiv - Cell Biology 2022Quote: ... and calyculin A serine/threonine phosphatase inhibitor (10µL per mL of volume; Cell Signaling). Ovaries were homogenized on ice by grinding with an automated tissue grinder (KONTES pellet pestle ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclear counterstaining was performed using 1 μg/ml Hoechst 33342 (no. 4082; Cell Signaling) at room temperature for 15 min in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... or 0.4 μL isotype control (2.5 mg/mL, Cell Signaling Technology, Danvers, United States) were added ...
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bioRxiv - Bioengineering 2021Quote: ... and then with rabbit anti-phospho Src Tyr416 (0.69 μg/mL, #2101, Cell Signaling) for overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The DEA-insoluble pellet was extracted with 0.5 ml RIPA buffer (Cell Signaling 9806S), plus protease phosphatase inhibitors ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated with 5 mL of 1x RBC lysis buffer (Cell signaling, 46232S) for 5 minutes on ice to remove red blood cells ...
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bioRxiv - Neuroscience 2023Quote: ... anti-phospho-IRF3 S396 (0.046 µg/ml, Cat. # 4947, RRID: AB_823547; Cell Signaling Technology), anti-TBK1/NAK (0.19 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-β-Actin (0.03 µg/ml, Cat. # 4967, RRID: AB_330288; Cell Signaling Technology). Next ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml; 2144, Cell Signaling Technology) overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Hearts were homogenized in cell lysis buffer (1 mL; Cell Signaling Technology, Danvers, MA) supplemented with a protease and phosphatase-inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... Mice were treated with 20μl of 0.2mg/ml of TPA (Cell Signaling Technology #4174S), topically on the dorsal side of ear skin ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... The Developmental Studies Hybridoma Bank at the University of Iowa) and Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) (1:100; Cell Signaling Technologies). Secondary antibodies were used at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved caspase-3 was probed in MuSCs by incubating the cells overnight in 1:100 Cleaved Caspase-3 (Asp175) (5A1E) rabbit mAb (Cell Signaling Technology). MuSCs were stained for 3 hours with 1:500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... followed by blocking with PBS containing 5% FBS for 10 min and incubation with a 1:50 dilution of anti-pSyk-AF647 (C87C1) or anti-pTyr-AF647 (P-Tyr-100) and anti-CD45-AF488 (D3F8Q, Cell Signaling Technology) overnight at 4°C with gentle rocking ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Developmental Biology 2020Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Cell Signaling Technology, #3195, 1:100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc. ...
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bioRxiv - Developmental Biology 2020Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoprecipitation was performed with 2 µg rabbit polyclonal anti-KDM2B (gift from Dr. Robert Klose, Oxford University) and rabbit anti-RING1B antibody (1:100, Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with the anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) (1:100, 13820S, Cell Signaling Technology), anti-HA (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66, Sigma;p-Tyr-100, Cell Signaling Technology).
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bioRxiv - Neuroscience 2019Quote: ... Cells then underwent immunocytochemical processing similar to that described above using primary antibodies against activated c-Jun-N-terminal kinase (rabbit anti-pJNK, 1:100, Cell Signaling Technology) or phosphorylated p38 MAPK (rabbit anti-p-p38 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Cell Biology 2019Quote: ... 1:100 for IF) and Rabbit anti-GFP (#2956, 1:100 for IF and 1:1000 for WB, respectively) were from Cell Signaling Technology.
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bioRxiv - Cell Biology 2022Quote: Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-Phospho-JNK polyclonal antibody (Cell Signaling Technology, Cat #4668, 1:100), chicken anti-β-galactosidase antibody (Abcam ...
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bioRxiv - Immunology 2022Quote: ... intracellular immunostaining for total phospho-Tyrosine was performed by incubating with phospho-tyrosine mAb (P-Tyr-100 Alexa Fluor 647, Cell Signaling Technology). Cells were washed twice with and resuspended in FACS buffer and analyzed using a BD FACS Canto II flow cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween-20 in PBS) for at least 1 h and then incubated in 1:100 anti-pSmad1/5/9 (Cell Signaling, #13820) in FBS-PBST at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase inhibition and blocking with normal horse serum was also carried out before the incubation with primary antibodies (anti-rabbit caspase3, 1:100, Cell signaling #9662), anti-mouse ki67 ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were permeabilized in 0.2% Triton X-100 for 10 min at room temperature following the incubation with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were permeabilized in 0.2% Triton X-100 for 10 mins at room temperature then incubated with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with ice-cold PBS and lysed in 100 µL of phosphatase and protease inhibitor containing RIPA buffer (Cell Signaling Technology). Cells were incubated on ice for 30 min and then harvested by scraping followed by centrifugation at 14,000 rpm for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in FACS buffer and used 1:100 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058) and 1:500 for anti-rabbit Alexa-647 (goat ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Immunology 2023Quote: ... A 1:100 dilution of primary antibody (anti-UTX Cell Signaling Rabbit mAb #33510 or IgG Isotype Control: Cell Signaling Technology #3900S) in antibody buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-α-tubulin DM1α conjugated to Alexa Fluor 488 (1:100, Cell Signaling Technologies 8058S), mouse anti-centrin clone 20H5 (1:200 ...