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Citations for Cell Signaling Technology :
1151 - 1200 of 1211 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... membranes were treated with stripping buffer for 30 minutes and subsequently re-probed with a rabbit anti-GAPDH primary antibody (14C10; 1:20,000 dilution; Cell Signaling Technology) followed by the same HRP-conjugated secondary antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were anti-rabbit or anti-mouse IgG (H+L) (Dylight 680 or 800 conjugates) (Cell Signaling Technology). The membranes were scanned with the Odyssey infrared imaging system (LI-COR Biosciences).
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bioRxiv - Microbiology 2023Quote: ... and horseradish peroxidase (HRP)-labeled anti-mouse IgG (H + L) (cat #, 7076S, 1: 2,000, Cell Signaling Technology, Danvers, MA, USA) were used as primary and secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and goat anti-mouse IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 488 (Cell Signaling Technology, #4408). Each well ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and incubated with a 30 μl bed volume of protein-A-sepharose beads and 1.5 μl of α-IGF-1 receptor β (D23H3) XP (Cell Signaling) per sample ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated for 30 minutes on ice with a 1/30 dilution of medium containing RBD-sfGFP and a 1/240 dilution of anti-myc Alexa 647 (clone 9B11, Cell Signaling Technology). Cells were washed twice with PBS-BSA and analyzed on a BD LSR II ...
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4 % paraformaldehyde and 0.2 % picric acid in phosphate buffer (pH 6.9) for 30 minutes at room temperature and incubated with peroxidase-conjugated anti-rabbit antibody (Cell Signaling technology #7074) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were blocked with 5% goat serum in tris-buffered saline and 0.1% Tween® 20 detergent (TBST) for 30 min and stained with anti-HER2 antibodies at 1:500 dilution (Cell Signaling #21650). After three washes with TBST ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... were obtained at 30 h pt and incubated overnight with the anti-CD2v monoclonal antibody mix or anti-HA antibody (3724; Cell Signaling Technology). Pull down products were obtained by eluting in Laemmli buffer (Bio-Rad ...
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bioRxiv - Pathology 2021Quote: ... was used as secondary for 30 minutes and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling, Danvers, MA).
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody rabbit anti–Calreticulin at 1:100 (Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with a rabbit polyclonal anti‐pERK antibody (Cell Signaling; 9101L, lot #30, 1:1000), and mouse anti-NF200 (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: EV and mixed samples were vortexed for 30s and incubated for 10min at room temperature in 1X RIPA buffer (Cell Signaling 9806S) and 1X Protease inhibitors (Santa Cruz sc-29131 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pulled-down proteins were visualized by anti-FLAG Western blotting as described above (30, 58) (using anti-FLAG antiserum at 1:1000, #2368S, Cell Signaling Technology). To assess the amounts and sizes of the purified GST proteins used in the pulldowns ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in ice-cold 70% ethanol for at least 30 minutes and were then labeled with propidium iodide (PI)/RNase staining solution (#4087, Cell Signaling Technology) and further incubated for 15 minutes at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Neuroscience 2023Quote: ... neurons and mouse brain tissues were lysed for 30 minutes on ice in appropriate volume of cold RIPA lysis buffer (Cell Signaling Technologies) supplemented with protease inhibitor cocktail ...
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bioRxiv - Microbiology 2022Quote: ... The secondary antibodies used for immunoblotting were anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) (Cell Signaling, 5151S) and anti-mouse IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Immunology 2022Quote: MonoMac-6 cells were first washed with PBS and then lysed on ice for 30 min with immunoprecipitation (IP) lysis buffer (1x cell lysis buffer, Cell Signaling, cat#9803), 100 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 4% BSA in Tris-buffered saline containing 0.1% Tween-20 detergent (TBST) for 30 minutes then incubated overnight with PKm1 (Cell Signaling #4053; 1:1000), PKm2 (Cell Signaling #7067 ...
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bioRxiv - Genetics 2021Quote: ... Membranes were rinsed with TBST for 30 min before incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling; 7074S, 1:5000) for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1β (30 ng/ml, Cell Signaling Technology, 8900SF) and TNF-α (30 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue constructs were washed with 1× PBS 3 times and incubated for 30 min at 37°C in 1 mL staining solution containing 0.1% (v/v) Hoechst 33342 solution (Cell Signaling Technology, MA, USA) and 0.1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well Meso Scale Discovery assay plates (MSD, L15XA-6) were coated with 30 μl of pS65-Ub capturing antibody (Cell Signaling Technology, 62802) with a final concentration of 1 µg/ml in 200 mM sodium carbonate coating buffer (pH 9.7 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were permeabilized in 0.25% Triton X-100 in PBS for 5 minutes and blocked in 10% goat serum/0.1% Triton X-100 in PBS for 30 minutes and then placed in the primary antibody overnight at RT (γH2AX, Cell Signaling, 1:500). The next morning ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo) for 30 minutes followed by staining with GATA6-PE (Cell Signaling Technologies) at 1:400 for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... containing 5% bovine serum albumin (BSA) for 30 min and then incubated with primary antibodies against p-ERK (#4370S, 1:1000, Cell Signaling, Danvers, MA), p-P38 (#4511S ...
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bioRxiv - Biochemistry 2023Quote: ... Blots were incubated with rhodopsin (clone B6-30, a kind gift from Dr. Paul Hargrave (20) and alpha-tubulin (Cell Signaling CAT #2125) primary antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... proteins were extracted from the heads of 30-50 wildtype and mdkami5001 embryos in cold RIPA lysis buffer containing protease and phosphatase inhibitor cocktail (Cell Signaling Tec., Danvers, MA). Proteins were separated in 12%Mini-PROTEIN TGX Precast gel (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 0.5% BSA–PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (30 ng/ml) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... Cells were blocked with 5 mg/ml BSA in 1X PBS for 30 minutes and then incubated with primary antibody phospho-histone H2AX Ser139 20E3 (Cell Signaling #9718, 1:500) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with the following primary antibodies for 30 minutes at room temperature: anti-Vimentin (1:100 dilution; Cell Signaling Technology, cat. 5741), anti-Epcam (1:100 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... The following secondary antibodies and their respective dilutions were used: Anti-mouse IgG(H+L) DyLight 800 conjugate (Cell Signaling Technologies, #5257), 1:20000 ...
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bioRxiv - Cancer Biology 2020Quote: ... DyLight 800 conjugate (1:15000, #5257) and anti-rabbit IgG(H+L) DyLight 680 conjugate (1:15000, #5366) from Cell Signaling Technologies. The images were captured using Odyssey CLx Imager (LICOR Biosciences).
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed with TBS-T before staining with secondary anti-rabbit IgG (H+L) DyLight 800 4X PEG Conjugate (Cell Signaling #5151) at 1:20,000 at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were blocked with 10% goat serum plus 1% BSA at room temperature for 30 minutes and then incubated with the primary antibody against Ki67 (Cell signaling, Cat #12202S, 1:800) or c-Myc (Cell signaling ...
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bioRxiv - Cell Biology 2021Quote: ... 2% normal goat serum and 0.2% Triton-X100 in PBS) for 30 minutes and hybridized with polyclonal rabbit anti-phospho-Histone H3 (Ser10) antibody (Cell Signaling Technology, #9701; 1:1000) at overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...